Cargando…

Scribble basal polarity acquisition in RPE cells and its mislocalization in a pathological AMD-like model

Apicobasal polarity is a hallmark of retinal pigment epithelium cells and is required to perform their functions; however, the precise roles of the different proteins that execute polarity are still poorly understood. Here, we have studied the expression and location of Scribble, the core member of...

Descripción completa

Detalles Bibliográficos
Autores principales: Segurado, Alicia, Rodríguez-Carrillo, Alba, Castellanos, Bárbara, Hernández-Galilea, Emiliano, Velasco, Almudena, Lillo, Concepción
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9539273/
https://www.ncbi.nlm.nih.gov/pubmed/36213611
http://dx.doi.org/10.3389/fnana.2022.983151
_version_ 1784803456598409216
author Segurado, Alicia
Rodríguez-Carrillo, Alba
Castellanos, Bárbara
Hernández-Galilea, Emiliano
Velasco, Almudena
Lillo, Concepción
author_facet Segurado, Alicia
Rodríguez-Carrillo, Alba
Castellanos, Bárbara
Hernández-Galilea, Emiliano
Velasco, Almudena
Lillo, Concepción
author_sort Segurado, Alicia
collection PubMed
description Apicobasal polarity is a hallmark of retinal pigment epithelium cells and is required to perform their functions; however, the precise roles of the different proteins that execute polarity are still poorly understood. Here, we have studied the expression and location of Scribble, the core member of the polarity basal protein complex in epithelial-derived cells, in human and mouse RPE cells in both control and pathological conditions. We found that Scribble specifically localizes at the basolateral membrane of mouse and human RPE cells. In addition, we observed an increase in the expression of Scribble during human RPE development in culture, while it acquires a well-defined basolateral pattern as this process is completed. Finally, the expression and location of Scribble were analyzed in human RPE cells in experimental conditions that mimic the toxic environment suffered by these cells during AMD development and found an increase in Scribble expression in cells that develop a pathological phenotype, suggesting that the protein could be altered in cells under stress conditions, as occurs in AMD. Together, our results demonstrate, for the first time, that Scribble is expressed in both human and mouse RPE and is localized at the basolateral membrane in mature cells. Furthermore, Scribble shows impaired expression and location in RPE cells in pathological conditions, suggesting a possible role for this protein in the development of pathologies, such as AMD.
format Online
Article
Text
id pubmed-9539273
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-95392732022-10-08 Scribble basal polarity acquisition in RPE cells and its mislocalization in a pathological AMD-like model Segurado, Alicia Rodríguez-Carrillo, Alba Castellanos, Bárbara Hernández-Galilea, Emiliano Velasco, Almudena Lillo, Concepción Front Neuroanat Neuroscience Apicobasal polarity is a hallmark of retinal pigment epithelium cells and is required to perform their functions; however, the precise roles of the different proteins that execute polarity are still poorly understood. Here, we have studied the expression and location of Scribble, the core member of the polarity basal protein complex in epithelial-derived cells, in human and mouse RPE cells in both control and pathological conditions. We found that Scribble specifically localizes at the basolateral membrane of mouse and human RPE cells. In addition, we observed an increase in the expression of Scribble during human RPE development in culture, while it acquires a well-defined basolateral pattern as this process is completed. Finally, the expression and location of Scribble were analyzed in human RPE cells in experimental conditions that mimic the toxic environment suffered by these cells during AMD development and found an increase in Scribble expression in cells that develop a pathological phenotype, suggesting that the protein could be altered in cells under stress conditions, as occurs in AMD. Together, our results demonstrate, for the first time, that Scribble is expressed in both human and mouse RPE and is localized at the basolateral membrane in mature cells. Furthermore, Scribble shows impaired expression and location in RPE cells in pathological conditions, suggesting a possible role for this protein in the development of pathologies, such as AMD. Frontiers Media S.A. 2022-09-23 /pmc/articles/PMC9539273/ /pubmed/36213611 http://dx.doi.org/10.3389/fnana.2022.983151 Text en Copyright © 2022 Segurado, Rodríguez-Carrillo, Castellanos, Hernández-Galilea, Velasco and Lillo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Segurado, Alicia
Rodríguez-Carrillo, Alba
Castellanos, Bárbara
Hernández-Galilea, Emiliano
Velasco, Almudena
Lillo, Concepción
Scribble basal polarity acquisition in RPE cells and its mislocalization in a pathological AMD-like model
title Scribble basal polarity acquisition in RPE cells and its mislocalization in a pathological AMD-like model
title_full Scribble basal polarity acquisition in RPE cells and its mislocalization in a pathological AMD-like model
title_fullStr Scribble basal polarity acquisition in RPE cells and its mislocalization in a pathological AMD-like model
title_full_unstemmed Scribble basal polarity acquisition in RPE cells and its mislocalization in a pathological AMD-like model
title_short Scribble basal polarity acquisition in RPE cells and its mislocalization in a pathological AMD-like model
title_sort scribble basal polarity acquisition in rpe cells and its mislocalization in a pathological amd-like model
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9539273/
https://www.ncbi.nlm.nih.gov/pubmed/36213611
http://dx.doi.org/10.3389/fnana.2022.983151
work_keys_str_mv AT seguradoalicia scribblebasalpolarityacquisitioninrpecellsanditsmislocalizationinapathologicalamdlikemodel
AT rodriguezcarrilloalba scribblebasalpolarityacquisitioninrpecellsanditsmislocalizationinapathologicalamdlikemodel
AT castellanosbarbara scribblebasalpolarityacquisitioninrpecellsanditsmislocalizationinapathologicalamdlikemodel
AT hernandezgalileaemiliano scribblebasalpolarityacquisitioninrpecellsanditsmislocalizationinapathologicalamdlikemodel
AT velascoalmudena scribblebasalpolarityacquisitioninrpecellsanditsmislocalizationinapathologicalamdlikemodel
AT lilloconcepcion scribblebasalpolarityacquisitioninrpecellsanditsmislocalizationinapathologicalamdlikemodel