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Diallyl trisulfide plays an antifibrotic role by inhibiting the expression of Bcl‐2 in hepatic stellate cells
Hepatic fibrosis is an important early stage in the evolution of liver cirrhosis, and specific medicine and therapeutic measures are unavailable to date. Hepatic stellate cells (HSCs) are the main cells involved in the formation of hepatic fibrosis, and induction of the apoptosis of HSCs is an impor...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9539501/ https://www.ncbi.nlm.nih.gov/pubmed/35532220 http://dx.doi.org/10.1002/jbt.23097 |
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author | Pang, Huai Wang, Cuizhe Ye, Jing Wang, Lulu Zhou, Xiaoming Ge, Xiaomeng Zhang, Jun Liu, Qinghua |
author_facet | Pang, Huai Wang, Cuizhe Ye, Jing Wang, Lulu Zhou, Xiaoming Ge, Xiaomeng Zhang, Jun Liu, Qinghua |
author_sort | Pang, Huai |
collection | PubMed |
description | Hepatic fibrosis is an important early stage in the evolution of liver cirrhosis, and specific medicine and therapeutic measures are unavailable to date. Hepatic stellate cells (HSCs) are the main cells involved in the formation of hepatic fibrosis, and induction of the apoptosis of HSCs is an important strategy for the treatment of hepatic fibrosis. Diallyl trisulfide (DATS) is a natural product and is the main active ingredient in garlic. However, the exact molecular mechanisms underlying HSC apoptosis induced by DATS are not well understood. This study aimed to analyze the efficiency and mechanism of DATS in hepatic fibrosis. Different concentrations (25, 50, 100, and 200 μM) of DATS were used to treat HSCs. Changes in cell morphology and formation of apoptotic bodies were observed under an inverted microscope and an electric microscope. Bcl‐2 signaling involving Bax, Caspase‐3, Caspase‐6, Caspase‐8, Caspase‐9, p53, Apaf‐1, and Cyto‐c in fibrosis were examined, which is a critical step in the evaluation of antihepatic fibrosis agents. We also evaluated the effect of DATS on the cellular morphology of HSCs and apoptosis‐related factors under different Bcl‐2 expression states. Our results suggest that DATS regulates hepatic fibrosis by blocking the Bcl‐2 signaling pathway and upregulating the Bax/Bcl‐2 ratio. |
format | Online Article Text |
id | pubmed-9539501 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95395012022-10-14 Diallyl trisulfide plays an antifibrotic role by inhibiting the expression of Bcl‐2 in hepatic stellate cells Pang, Huai Wang, Cuizhe Ye, Jing Wang, Lulu Zhou, Xiaoming Ge, Xiaomeng Zhang, Jun Liu, Qinghua J Biochem Mol Toxicol Research Articles Hepatic fibrosis is an important early stage in the evolution of liver cirrhosis, and specific medicine and therapeutic measures are unavailable to date. Hepatic stellate cells (HSCs) are the main cells involved in the formation of hepatic fibrosis, and induction of the apoptosis of HSCs is an important strategy for the treatment of hepatic fibrosis. Diallyl trisulfide (DATS) is a natural product and is the main active ingredient in garlic. However, the exact molecular mechanisms underlying HSC apoptosis induced by DATS are not well understood. This study aimed to analyze the efficiency and mechanism of DATS in hepatic fibrosis. Different concentrations (25, 50, 100, and 200 μM) of DATS were used to treat HSCs. Changes in cell morphology and formation of apoptotic bodies were observed under an inverted microscope and an electric microscope. Bcl‐2 signaling involving Bax, Caspase‐3, Caspase‐6, Caspase‐8, Caspase‐9, p53, Apaf‐1, and Cyto‐c in fibrosis were examined, which is a critical step in the evaluation of antihepatic fibrosis agents. We also evaluated the effect of DATS on the cellular morphology of HSCs and apoptosis‐related factors under different Bcl‐2 expression states. Our results suggest that DATS regulates hepatic fibrosis by blocking the Bcl‐2 signaling pathway and upregulating the Bax/Bcl‐2 ratio. John Wiley and Sons Inc. 2022-05-09 2022-08 /pmc/articles/PMC9539501/ /pubmed/35532220 http://dx.doi.org/10.1002/jbt.23097 Text en © 2022 The Authors. Journal of Biochemical and Molecular Toxicology published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Pang, Huai Wang, Cuizhe Ye, Jing Wang, Lulu Zhou, Xiaoming Ge, Xiaomeng Zhang, Jun Liu, Qinghua Diallyl trisulfide plays an antifibrotic role by inhibiting the expression of Bcl‐2 in hepatic stellate cells |
title | Diallyl trisulfide plays an antifibrotic role by inhibiting the expression of Bcl‐2 in hepatic stellate cells |
title_full | Diallyl trisulfide plays an antifibrotic role by inhibiting the expression of Bcl‐2 in hepatic stellate cells |
title_fullStr | Diallyl trisulfide plays an antifibrotic role by inhibiting the expression of Bcl‐2 in hepatic stellate cells |
title_full_unstemmed | Diallyl trisulfide plays an antifibrotic role by inhibiting the expression of Bcl‐2 in hepatic stellate cells |
title_short | Diallyl trisulfide plays an antifibrotic role by inhibiting the expression of Bcl‐2 in hepatic stellate cells |
title_sort | diallyl trisulfide plays an antifibrotic role by inhibiting the expression of bcl‐2 in hepatic stellate cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9539501/ https://www.ncbi.nlm.nih.gov/pubmed/35532220 http://dx.doi.org/10.1002/jbt.23097 |
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