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Increased proportions of inflammatory T cells and their correlations with cytokines and clinical parameters in patients with ankylosing spondylitis from northern Sweden

Ankylosing spondylitis (AS) is an autoimmune disease affecting parts of the skeletal structure in particular. Previously increased levels of the inflammatory cell types Th17, Th22, Tc17 and Tc22 cells have been shown to be associated with AS. Here, we analysed the levels of inflammatory T cell subse...

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Autores principales: Lejon, Kristina, Hellman, Urban, Do, Lan, Kumar, Anjani, Forsblad‐d’Elia, Helena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9539637/
https://www.ncbi.nlm.nih.gov/pubmed/35506752
http://dx.doi.org/10.1111/sji.13190
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author Lejon, Kristina
Hellman, Urban
Do, Lan
Kumar, Anjani
Forsblad‐d’Elia, Helena
author_facet Lejon, Kristina
Hellman, Urban
Do, Lan
Kumar, Anjani
Forsblad‐d’Elia, Helena
author_sort Lejon, Kristina
collection PubMed
description Ankylosing spondylitis (AS) is an autoimmune disease affecting parts of the skeletal structure in particular. Previously increased levels of the inflammatory cell types Th17, Th22, Tc17 and Tc22 cells have been shown to be associated with AS. Here, we analysed the levels of inflammatory T cell subsets, related cytokines and clinical characteristics of AS patients vs controls from northern Sweden. Peripheral blood mononuclear cells (PBMCs) obtained from 50 AS patients and 50 matched controls were short term stimulated with PMA/Ionomycin, stained and analysed by flow cytometry. Plasma levels of Interleukin (IL)‐17, IL‐22, IL‐10 as well as clinically relevant markers were determined. Compared to male controls, male AS patients showed 1.5‐ to 2‐fold increases of Th17 (P = .013), Th22 (P = .003) and Tc22 (P = .024) among CD45(+)CD3(+) lymphocytes. Plasma IL‐22 levels correlated with the Tc17 proportion in male patients (R(s) = 0.499, P = .003) and plasma IL‐10 levels were inversely correlated with Tc17 among all patients (R(s) = −0.276, P = .05). Male patients with syndesmophytes showed significantly higher Th17 proportions (P = .038). In female AS patients, Tc22 was negatively correlated with C‐reactive protein (high sensitivity) (hsCRP) (R(s) = −0.573, P = .016). We confirmed increased proportions of inflammatory T cells and correlations with relevant cytokines from male AS patients. The correlation between Th17 and syndesmophytes supports a role of Th17 in the pathogenic process.
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spelling pubmed-95396372022-10-14 Increased proportions of inflammatory T cells and their correlations with cytokines and clinical parameters in patients with ankylosing spondylitis from northern Sweden Lejon, Kristina Hellman, Urban Do, Lan Kumar, Anjani Forsblad‐d’Elia, Helena Scand J Immunol SCANDINAVIAN JOURNAL OF IMMUNOLOGY 50 YEARS: 1972 ‐ 2022 Ankylosing spondylitis (AS) is an autoimmune disease affecting parts of the skeletal structure in particular. Previously increased levels of the inflammatory cell types Th17, Th22, Tc17 and Tc22 cells have been shown to be associated with AS. Here, we analysed the levels of inflammatory T cell subsets, related cytokines and clinical characteristics of AS patients vs controls from northern Sweden. Peripheral blood mononuclear cells (PBMCs) obtained from 50 AS patients and 50 matched controls were short term stimulated with PMA/Ionomycin, stained and analysed by flow cytometry. Plasma levels of Interleukin (IL)‐17, IL‐22, IL‐10 as well as clinically relevant markers were determined. Compared to male controls, male AS patients showed 1.5‐ to 2‐fold increases of Th17 (P = .013), Th22 (P = .003) and Tc22 (P = .024) among CD45(+)CD3(+) lymphocytes. Plasma IL‐22 levels correlated with the Tc17 proportion in male patients (R(s) = 0.499, P = .003) and plasma IL‐10 levels were inversely correlated with Tc17 among all patients (R(s) = −0.276, P = .05). Male patients with syndesmophytes showed significantly higher Th17 proportions (P = .038). In female AS patients, Tc22 was negatively correlated with C‐reactive protein (high sensitivity) (hsCRP) (R(s) = −0.573, P = .016). We confirmed increased proportions of inflammatory T cells and correlations with relevant cytokines from male AS patients. The correlation between Th17 and syndesmophytes supports a role of Th17 in the pathogenic process. John Wiley and Sons Inc. 2022-06-01 2022-09 /pmc/articles/PMC9539637/ /pubmed/35506752 http://dx.doi.org/10.1111/sji.13190 Text en © 2022 The Authors. Scandinavian Journal of Immunology published by John Wiley & Sons Ltd on behalf of The Scandinavian Foundation for Immunology. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle SCANDINAVIAN JOURNAL OF IMMUNOLOGY 50 YEARS: 1972 ‐ 2022
Lejon, Kristina
Hellman, Urban
Do, Lan
Kumar, Anjani
Forsblad‐d’Elia, Helena
Increased proportions of inflammatory T cells and their correlations with cytokines and clinical parameters in patients with ankylosing spondylitis from northern Sweden
title Increased proportions of inflammatory T cells and their correlations with cytokines and clinical parameters in patients with ankylosing spondylitis from northern Sweden
title_full Increased proportions of inflammatory T cells and their correlations with cytokines and clinical parameters in patients with ankylosing spondylitis from northern Sweden
title_fullStr Increased proportions of inflammatory T cells and their correlations with cytokines and clinical parameters in patients with ankylosing spondylitis from northern Sweden
title_full_unstemmed Increased proportions of inflammatory T cells and their correlations with cytokines and clinical parameters in patients with ankylosing spondylitis from northern Sweden
title_short Increased proportions of inflammatory T cells and their correlations with cytokines and clinical parameters in patients with ankylosing spondylitis from northern Sweden
title_sort increased proportions of inflammatory t cells and their correlations with cytokines and clinical parameters in patients with ankylosing spondylitis from northern sweden
topic SCANDINAVIAN JOURNAL OF IMMUNOLOGY 50 YEARS: 1972 ‐ 2022
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9539637/
https://www.ncbi.nlm.nih.gov/pubmed/35506752
http://dx.doi.org/10.1111/sji.13190
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