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Mild cold stress specifically disturbs clustering movement of DFCs and sequential organ left-right patterning in zebrafish
In poikilothermic animals, the distinct acclimatization ability of different organs has been previously addressed, while the tissue-specific role of cold stress in early development is largely unknown. In this study, we discovered that despite its role in delaying embryonic development, mild cold st...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9539758/ https://www.ncbi.nlm.nih.gov/pubmed/36211472 http://dx.doi.org/10.3389/fcell.2022.952844 |
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author | Liu, Min Zou, Xinyu Fu, Mao Bai, Xinping Zhao, Yongyan Chen, Xin Wang, Xiaoyu Wang, Peijian Huang, Sizhou |
author_facet | Liu, Min Zou, Xinyu Fu, Mao Bai, Xinping Zhao, Yongyan Chen, Xin Wang, Xiaoyu Wang, Peijian Huang, Sizhou |
author_sort | Liu, Min |
collection | PubMed |
description | In poikilothermic animals, the distinct acclimatization ability of different organs has been previously addressed, while the tissue-specific role of cold stress in early development is largely unknown. In this study, we discovered that despite its role in delaying embryonic development, mild cold stress (22°C) does not disturb multiple-organ progenitor specification, but does give rise to organ left-right (LR) patterning defects. Regarding the mechanism, the data showed that mild cold stress downregulated the expression of cell-adhesion genes cdh1 and cdh2 during gastrulation, especially in dorsal forerunner cells (DFCs), which partially disturbed the clustering movement of DFCs, Kupffer’s vesicle (KV) morphogenesis, and ciliogenesis. As a result, the defects of KV/cilia disrupted asymmetric nodal signaling and subsequent heart and liver LR patterning. In conclusion, our data novelly identified that, in early development, DFCs are more sensitive to mild cold stress, and mild cold stress repressed the expression of cell adhesion-related gene cdh1 and cdh2. This role partially disturbed the clustering movement of DFCs, which resulted in defective KV/cilia development and sequential organ LR patterning defects. |
format | Online Article Text |
id | pubmed-9539758 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95397582022-10-08 Mild cold stress specifically disturbs clustering movement of DFCs and sequential organ left-right patterning in zebrafish Liu, Min Zou, Xinyu Fu, Mao Bai, Xinping Zhao, Yongyan Chen, Xin Wang, Xiaoyu Wang, Peijian Huang, Sizhou Front Cell Dev Biol Cell and Developmental Biology In poikilothermic animals, the distinct acclimatization ability of different organs has been previously addressed, while the tissue-specific role of cold stress in early development is largely unknown. In this study, we discovered that despite its role in delaying embryonic development, mild cold stress (22°C) does not disturb multiple-organ progenitor specification, but does give rise to organ left-right (LR) patterning defects. Regarding the mechanism, the data showed that mild cold stress downregulated the expression of cell-adhesion genes cdh1 and cdh2 during gastrulation, especially in dorsal forerunner cells (DFCs), which partially disturbed the clustering movement of DFCs, Kupffer’s vesicle (KV) morphogenesis, and ciliogenesis. As a result, the defects of KV/cilia disrupted asymmetric nodal signaling and subsequent heart and liver LR patterning. In conclusion, our data novelly identified that, in early development, DFCs are more sensitive to mild cold stress, and mild cold stress repressed the expression of cell adhesion-related gene cdh1 and cdh2. This role partially disturbed the clustering movement of DFCs, which resulted in defective KV/cilia development and sequential organ LR patterning defects. Frontiers Media S.A. 2022-09-23 /pmc/articles/PMC9539758/ /pubmed/36211472 http://dx.doi.org/10.3389/fcell.2022.952844 Text en Copyright © 2022 Liu, Zou, Fu, Bai, Zhao, Chen, Wang, Wang and Huang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Liu, Min Zou, Xinyu Fu, Mao Bai, Xinping Zhao, Yongyan Chen, Xin Wang, Xiaoyu Wang, Peijian Huang, Sizhou Mild cold stress specifically disturbs clustering movement of DFCs and sequential organ left-right patterning in zebrafish |
title | Mild cold stress specifically disturbs clustering movement of DFCs and sequential organ left-right patterning in zebrafish |
title_full | Mild cold stress specifically disturbs clustering movement of DFCs and sequential organ left-right patterning in zebrafish |
title_fullStr | Mild cold stress specifically disturbs clustering movement of DFCs and sequential organ left-right patterning in zebrafish |
title_full_unstemmed | Mild cold stress specifically disturbs clustering movement of DFCs and sequential organ left-right patterning in zebrafish |
title_short | Mild cold stress specifically disturbs clustering movement of DFCs and sequential organ left-right patterning in zebrafish |
title_sort | mild cold stress specifically disturbs clustering movement of dfcs and sequential organ left-right patterning in zebrafish |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9539758/ https://www.ncbi.nlm.nih.gov/pubmed/36211472 http://dx.doi.org/10.3389/fcell.2022.952844 |
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