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Effects of light spectrum on the morphophysiology and gene expression of lateral branching in Pepino (Solanum muricatum)

In the present study, we determined the morphological and physiological indicators of Pepino to elucidate its lateral branching responses to different light qualities using a full-spectrum lamp (F) as the control and eight different light ratios using blue light (B) and red light (R). In addition, c...

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Autores principales: Si, Cheng, Yang, Shipeng, Lou, Xiangyun, Zhang, Guangnan, Zhong, Qiwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9540516/
https://www.ncbi.nlm.nih.gov/pubmed/36212344
http://dx.doi.org/10.3389/fpls.2022.1012086
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author Si, Cheng
Yang, Shipeng
Lou, Xiangyun
Zhang, Guangnan
Zhong, Qiwen
author_facet Si, Cheng
Yang, Shipeng
Lou, Xiangyun
Zhang, Guangnan
Zhong, Qiwen
author_sort Si, Cheng
collection PubMed
description In the present study, we determined the morphological and physiological indicators of Pepino to elucidate its lateral branching responses to different light qualities using a full-spectrum lamp (F) as the control and eight different light ratios using blue light (B) and red light (R). In addition, correlation analysis revealed that the gene expression patterns correlated with lateral branching under various light treatments. Compared with the F treatment, the R treatment increased the plant height and inhibited the elongation of lateral branches, in contrast with the B treatment. The number of lateral branches did not change significantly under different light quality treatments. Moreover, correlation analysis showed that the ratio of blue light was significantly positively correlated with the length of lateral branches and significantly negatively correlated with plant height, aboveground dry weight, and other indicators. We conducted transcriptome sequencing of the sites of lateral branching at three periods under different light quality treatments. The gene related to photodynamic response, cryptochrome (CRY), was the most highly expressed under B treatment, negatively regulated lateral branch length, and positively correlated with plant height. Branched 1, a lateral branch regulation gene, was upregulated under R treatment and inhibited branching. Overall, the red light facilitated internode elongation, leaf area expansion, plant dry weight increase, and inhibition of lateral branching. Soluble sugar content increased, and the lateral branches elongated under blue light. Different light qualities regulated lateral branching by mediating different pathways involving strigolactones and CRY. Our findings laid a foundation for further clarifying the response mechanism of Pepino seedlings to light and provided a theoretical reference for elucidating the regulation of different light qualities on the lateral branching of Pepino.
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spelling pubmed-95405162022-10-08 Effects of light spectrum on the morphophysiology and gene expression of lateral branching in Pepino (Solanum muricatum) Si, Cheng Yang, Shipeng Lou, Xiangyun Zhang, Guangnan Zhong, Qiwen Front Plant Sci Plant Science In the present study, we determined the morphological and physiological indicators of Pepino to elucidate its lateral branching responses to different light qualities using a full-spectrum lamp (F) as the control and eight different light ratios using blue light (B) and red light (R). In addition, correlation analysis revealed that the gene expression patterns correlated with lateral branching under various light treatments. Compared with the F treatment, the R treatment increased the plant height and inhibited the elongation of lateral branches, in contrast with the B treatment. The number of lateral branches did not change significantly under different light quality treatments. Moreover, correlation analysis showed that the ratio of blue light was significantly positively correlated with the length of lateral branches and significantly negatively correlated with plant height, aboveground dry weight, and other indicators. We conducted transcriptome sequencing of the sites of lateral branching at three periods under different light quality treatments. The gene related to photodynamic response, cryptochrome (CRY), was the most highly expressed under B treatment, negatively regulated lateral branch length, and positively correlated with plant height. Branched 1, a lateral branch regulation gene, was upregulated under R treatment and inhibited branching. Overall, the red light facilitated internode elongation, leaf area expansion, plant dry weight increase, and inhibition of lateral branching. Soluble sugar content increased, and the lateral branches elongated under blue light. Different light qualities regulated lateral branching by mediating different pathways involving strigolactones and CRY. Our findings laid a foundation for further clarifying the response mechanism of Pepino seedlings to light and provided a theoretical reference for elucidating the regulation of different light qualities on the lateral branching of Pepino. Frontiers Media S.A. 2022-09-23 /pmc/articles/PMC9540516/ /pubmed/36212344 http://dx.doi.org/10.3389/fpls.2022.1012086 Text en Copyright © 2022 Si, Yang, Lou, Zhang and Zhong https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Si, Cheng
Yang, Shipeng
Lou, Xiangyun
Zhang, Guangnan
Zhong, Qiwen
Effects of light spectrum on the morphophysiology and gene expression of lateral branching in Pepino (Solanum muricatum)
title Effects of light spectrum on the morphophysiology and gene expression of lateral branching in Pepino (Solanum muricatum)
title_full Effects of light spectrum on the morphophysiology and gene expression of lateral branching in Pepino (Solanum muricatum)
title_fullStr Effects of light spectrum on the morphophysiology and gene expression of lateral branching in Pepino (Solanum muricatum)
title_full_unstemmed Effects of light spectrum on the morphophysiology and gene expression of lateral branching in Pepino (Solanum muricatum)
title_short Effects of light spectrum on the morphophysiology and gene expression of lateral branching in Pepino (Solanum muricatum)
title_sort effects of light spectrum on the morphophysiology and gene expression of lateral branching in pepino (solanum muricatum)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9540516/
https://www.ncbi.nlm.nih.gov/pubmed/36212344
http://dx.doi.org/10.3389/fpls.2022.1012086
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