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Wolbachia wAlbB inhibits bluetongue and epizootic hemorrhagic fever viruses in Culicoides midge cells
Culicoides midges are hematophagous insects that transmit arboviruses of veterinary importance. These viruses include bluetongue virus (BTV) and epizootic hemorrhagic fever virus (EHDV). The endosymbiont Wolbachia pipientis Hertig spreads rapidly through insect host populations and has been demonstr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9540819/ https://www.ncbi.nlm.nih.gov/pubmed/35266572 http://dx.doi.org/10.1111/mve.12569 |
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author | Matthews, Megan L. Covey, Hunter O. Drolet, Barbara S. Brelsfoard, Corey L. |
author_facet | Matthews, Megan L. Covey, Hunter O. Drolet, Barbara S. Brelsfoard, Corey L. |
author_sort | Matthews, Megan L. |
collection | PubMed |
description | Culicoides midges are hematophagous insects that transmit arboviruses of veterinary importance. These viruses include bluetongue virus (BTV) and epizootic hemorrhagic fever virus (EHDV). The endosymbiont Wolbachia pipientis Hertig spreads rapidly through insect host populations and has been demonstrated to inhibit viral pathogen transmission in multiple mosquito vectors. Here, we have demonstrated a replication inhibitory effect on BTV and EHDV in a Wolbachia (wAlbB strain)‐infected Culicoides sonorensis Wirth and Jones W8 cell line. Viral replication was significantly reduced by day 5 for BTV and by day 2 for EHDV as detected by real‐time polymerase chain reaction (RT‐qPCR) of the non‐structural NS3 gene of both viruses. Evaluation of innate cellular immune responses as a cause of the inhibitory effect showed responses associated with BTV but not with EHDV infection. Wolbachia density also did not play a role in the observed pathogen inhibitory effects, and an alternative hypothesis is suggested. Applications of Wolbachia‐mediated pathogen interference to impact disease transmission by Culicoides midges are discussed. |
format | Online Article Text |
id | pubmed-9540819 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-95408192022-10-14 Wolbachia wAlbB inhibits bluetongue and epizootic hemorrhagic fever viruses in Culicoides midge cells Matthews, Megan L. Covey, Hunter O. Drolet, Barbara S. Brelsfoard, Corey L. Med Vet Entomol Regular Articles Culicoides midges are hematophagous insects that transmit arboviruses of veterinary importance. These viruses include bluetongue virus (BTV) and epizootic hemorrhagic fever virus (EHDV). The endosymbiont Wolbachia pipientis Hertig spreads rapidly through insect host populations and has been demonstrated to inhibit viral pathogen transmission in multiple mosquito vectors. Here, we have demonstrated a replication inhibitory effect on BTV and EHDV in a Wolbachia (wAlbB strain)‐infected Culicoides sonorensis Wirth and Jones W8 cell line. Viral replication was significantly reduced by day 5 for BTV and by day 2 for EHDV as detected by real‐time polymerase chain reaction (RT‐qPCR) of the non‐structural NS3 gene of both viruses. Evaluation of innate cellular immune responses as a cause of the inhibitory effect showed responses associated with BTV but not with EHDV infection. Wolbachia density also did not play a role in the observed pathogen inhibitory effects, and an alternative hypothesis is suggested. Applications of Wolbachia‐mediated pathogen interference to impact disease transmission by Culicoides midges are discussed. Blackwell Publishing Ltd 2022-03-10 2022-09 /pmc/articles/PMC9540819/ /pubmed/35266572 http://dx.doi.org/10.1111/mve.12569 Text en © 2022 The Authors. Medical and Veterinary Entomology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Regular Articles Matthews, Megan L. Covey, Hunter O. Drolet, Barbara S. Brelsfoard, Corey L. Wolbachia wAlbB inhibits bluetongue and epizootic hemorrhagic fever viruses in Culicoides midge cells |
title |
Wolbachia
wAlbB inhibits bluetongue and epizootic hemorrhagic fever viruses in Culicoides midge cells |
title_full |
Wolbachia
wAlbB inhibits bluetongue and epizootic hemorrhagic fever viruses in Culicoides midge cells |
title_fullStr |
Wolbachia
wAlbB inhibits bluetongue and epizootic hemorrhagic fever viruses in Culicoides midge cells |
title_full_unstemmed |
Wolbachia
wAlbB inhibits bluetongue and epizootic hemorrhagic fever viruses in Culicoides midge cells |
title_short |
Wolbachia
wAlbB inhibits bluetongue and epizootic hemorrhagic fever viruses in Culicoides midge cells |
title_sort | wolbachia
walbb inhibits bluetongue and epizootic hemorrhagic fever viruses in culicoides midge cells |
topic | Regular Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9540819/ https://www.ncbi.nlm.nih.gov/pubmed/35266572 http://dx.doi.org/10.1111/mve.12569 |
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