Cargando…

PMEL p.Leu18del dilutes coat color of Kumamoto sub-breed of Japanese Brown cattle

BACKGROUND: Coat color is important for registration and maintenance of livestock. Standard coat color of Kumamoto sub-breed of Japanese Brown cattle is solid brown, but individuals with diluted coat color have been observed recently. In this study, we attempted to identify polymorphism(s) responsib...

Descripción completa

Detalles Bibliográficos
Autores principales: Kimura, Satoshi, Hatakeyama, Touko, Koutaka, Takashi, Kubo, Kazuhiro, Morita, Satoru, Eguchi, Keiko, Saitoh, Kohji, Yamauchi, Kenji, Imai, Saki, Kashimura, Atsushi, Inenaga, Toshiaki, Matsumoto, Hirokazu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9541072/
https://www.ncbi.nlm.nih.gov/pubmed/36207673
http://dx.doi.org/10.1186/s12864-022-08916-8
_version_ 1784803844920705024
author Kimura, Satoshi
Hatakeyama, Touko
Koutaka, Takashi
Kubo, Kazuhiro
Morita, Satoru
Eguchi, Keiko
Saitoh, Kohji
Yamauchi, Kenji
Imai, Saki
Kashimura, Atsushi
Inenaga, Toshiaki
Matsumoto, Hirokazu
author_facet Kimura, Satoshi
Hatakeyama, Touko
Koutaka, Takashi
Kubo, Kazuhiro
Morita, Satoru
Eguchi, Keiko
Saitoh, Kohji
Yamauchi, Kenji
Imai, Saki
Kashimura, Atsushi
Inenaga, Toshiaki
Matsumoto, Hirokazu
author_sort Kimura, Satoshi
collection PubMed
description BACKGROUND: Coat color is important for registration and maintenance of livestock. Standard coat color of Kumamoto sub-breed of Japanese Brown cattle is solid brown, but individuals with diluted coat color have been observed recently. In this study, we attempted to identify polymorphism(s) responsible for coat color dilution by whole genome analysis. RESULTS: One of the diluted cattle possessed 7302 exonic polymorphisms which could affect genes’ function. Among them, 14 polymorphisms in 10 coat color-related genes were assumed to be specific for the diluted cattle. Subsequent genotyping with three diluted cattle and 74 standard cattle elucidated that PMEL p.Leu18del was the causative polymorphism for coat color dilution in this sub-breed. Individuals with del/del type of this polymorphism showed diluted coat color, but coat color of heterozygotes were intermediate with various dilution rates. CONCLUSIONS: Coat color dilution of Kumamoto sub-breed was caused by PMEL p.Leu18del. The causative del allele has been detected in several genetically distant cattle breeds, suggesting that PMEL p.Leu18del can be used as a DNA marker to control cattle coat color. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-08916-8.
format Online
Article
Text
id pubmed-9541072
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-95410722022-10-08 PMEL p.Leu18del dilutes coat color of Kumamoto sub-breed of Japanese Brown cattle Kimura, Satoshi Hatakeyama, Touko Koutaka, Takashi Kubo, Kazuhiro Morita, Satoru Eguchi, Keiko Saitoh, Kohji Yamauchi, Kenji Imai, Saki Kashimura, Atsushi Inenaga, Toshiaki Matsumoto, Hirokazu BMC Genomics Research BACKGROUND: Coat color is important for registration and maintenance of livestock. Standard coat color of Kumamoto sub-breed of Japanese Brown cattle is solid brown, but individuals with diluted coat color have been observed recently. In this study, we attempted to identify polymorphism(s) responsible for coat color dilution by whole genome analysis. RESULTS: One of the diluted cattle possessed 7302 exonic polymorphisms which could affect genes’ function. Among them, 14 polymorphisms in 10 coat color-related genes were assumed to be specific for the diluted cattle. Subsequent genotyping with three diluted cattle and 74 standard cattle elucidated that PMEL p.Leu18del was the causative polymorphism for coat color dilution in this sub-breed. Individuals with del/del type of this polymorphism showed diluted coat color, but coat color of heterozygotes were intermediate with various dilution rates. CONCLUSIONS: Coat color dilution of Kumamoto sub-breed was caused by PMEL p.Leu18del. The causative del allele has been detected in several genetically distant cattle breeds, suggesting that PMEL p.Leu18del can be used as a DNA marker to control cattle coat color. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-08916-8. BioMed Central 2022-10-07 /pmc/articles/PMC9541072/ /pubmed/36207673 http://dx.doi.org/10.1186/s12864-022-08916-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Kimura, Satoshi
Hatakeyama, Touko
Koutaka, Takashi
Kubo, Kazuhiro
Morita, Satoru
Eguchi, Keiko
Saitoh, Kohji
Yamauchi, Kenji
Imai, Saki
Kashimura, Atsushi
Inenaga, Toshiaki
Matsumoto, Hirokazu
PMEL p.Leu18del dilutes coat color of Kumamoto sub-breed of Japanese Brown cattle
title PMEL p.Leu18del dilutes coat color of Kumamoto sub-breed of Japanese Brown cattle
title_full PMEL p.Leu18del dilutes coat color of Kumamoto sub-breed of Japanese Brown cattle
title_fullStr PMEL p.Leu18del dilutes coat color of Kumamoto sub-breed of Japanese Brown cattle
title_full_unstemmed PMEL p.Leu18del dilutes coat color of Kumamoto sub-breed of Japanese Brown cattle
title_short PMEL p.Leu18del dilutes coat color of Kumamoto sub-breed of Japanese Brown cattle
title_sort pmel p.leu18del dilutes coat color of kumamoto sub-breed of japanese brown cattle
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9541072/
https://www.ncbi.nlm.nih.gov/pubmed/36207673
http://dx.doi.org/10.1186/s12864-022-08916-8
work_keys_str_mv AT kimurasatoshi pmelpleu18deldilutescoatcolorofkumamotosubbreedofjapanesebrowncattle
AT hatakeyamatouko pmelpleu18deldilutescoatcolorofkumamotosubbreedofjapanesebrowncattle
AT koutakatakashi pmelpleu18deldilutescoatcolorofkumamotosubbreedofjapanesebrowncattle
AT kubokazuhiro pmelpleu18deldilutescoatcolorofkumamotosubbreedofjapanesebrowncattle
AT moritasatoru pmelpleu18deldilutescoatcolorofkumamotosubbreedofjapanesebrowncattle
AT eguchikeiko pmelpleu18deldilutescoatcolorofkumamotosubbreedofjapanesebrowncattle
AT saitohkohji pmelpleu18deldilutescoatcolorofkumamotosubbreedofjapanesebrowncattle
AT yamauchikenji pmelpleu18deldilutescoatcolorofkumamotosubbreedofjapanesebrowncattle
AT imaisaki pmelpleu18deldilutescoatcolorofkumamotosubbreedofjapanesebrowncattle
AT kashimuraatsushi pmelpleu18deldilutescoatcolorofkumamotosubbreedofjapanesebrowncattle
AT inenagatoshiaki pmelpleu18deldilutescoatcolorofkumamotosubbreedofjapanesebrowncattle
AT matsumotohirokazu pmelpleu18deldilutescoatcolorofkumamotosubbreedofjapanesebrowncattle