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The MBS microbial rapid detection system for rapid detection of major pathogenic bacteria in feed: comparison with plate counting method

The current methods for detecting pathogenic bacteria in feed require high technique and take a long time. The Micro Biological Survey (MBS) rapid detection system is a simple, economical and rapid microbial detection method. The purpose of this experiment was to compare the detection of Escherichia...

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Autores principales: Jiang, Linlin, Zhang, Beibei, Liu, Shuitao, Zhu, Lianqin, Zhu, Fenghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9541081/
https://www.ncbi.nlm.nih.gov/pubmed/36203160
http://dx.doi.org/10.1186/s12866-022-02655-2
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author Jiang, Linlin
Zhang, Beibei
Liu, Shuitao
Zhu, Lianqin
Zhu, Fenghua
author_facet Jiang, Linlin
Zhang, Beibei
Liu, Shuitao
Zhu, Lianqin
Zhu, Fenghua
author_sort Jiang, Linlin
collection PubMed
description The current methods for detecting pathogenic bacteria in feed require high technique and take a long time. The Micro Biological Survey (MBS) rapid detection system is a simple, economical and rapid microbial detection method. The purpose of this experiment was to compare the detection of Escherichia coli (E. coli), Salmonella, Staphylococcus aureus (S. aureus), Listeria monocytogenes (LM), coliform (COLI) and total viable count (TVC) in feed by the MBS rapid microbial detection system and plate counting method (PCM). The results showed that the limit of quantitation, recovery rate and coefficient of variation of the MBS microbial rapid detection system are better than the plate counting method. When detecting the pathogenic bacteria content in artificially contaminated feed, the MBS rapid microbial detection system was positively correlated with the PCM. When the MBS microbial rapid detection system and PCM were used to detect the collected real feed samples, there was no significant difference in the detection results of the two methods in most of the feed samples. In summary, the MBS microbial rapid detection system is the most convenient and rapid detection method and is suitable for promotion and application in production lines.
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spelling pubmed-95410812022-10-08 The MBS microbial rapid detection system for rapid detection of major pathogenic bacteria in feed: comparison with plate counting method Jiang, Linlin Zhang, Beibei Liu, Shuitao Zhu, Lianqin Zhu, Fenghua BMC Microbiol Research The current methods for detecting pathogenic bacteria in feed require high technique and take a long time. The Micro Biological Survey (MBS) rapid detection system is a simple, economical and rapid microbial detection method. The purpose of this experiment was to compare the detection of Escherichia coli (E. coli), Salmonella, Staphylococcus aureus (S. aureus), Listeria monocytogenes (LM), coliform (COLI) and total viable count (TVC) in feed by the MBS rapid microbial detection system and plate counting method (PCM). The results showed that the limit of quantitation, recovery rate and coefficient of variation of the MBS microbial rapid detection system are better than the plate counting method. When detecting the pathogenic bacteria content in artificially contaminated feed, the MBS rapid microbial detection system was positively correlated with the PCM. When the MBS microbial rapid detection system and PCM were used to detect the collected real feed samples, there was no significant difference in the detection results of the two methods in most of the feed samples. In summary, the MBS microbial rapid detection system is the most convenient and rapid detection method and is suitable for promotion and application in production lines. BioMed Central 2022-10-06 /pmc/articles/PMC9541081/ /pubmed/36203160 http://dx.doi.org/10.1186/s12866-022-02655-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Jiang, Linlin
Zhang, Beibei
Liu, Shuitao
Zhu, Lianqin
Zhu, Fenghua
The MBS microbial rapid detection system for rapid detection of major pathogenic bacteria in feed: comparison with plate counting method
title The MBS microbial rapid detection system for rapid detection of major pathogenic bacteria in feed: comparison with plate counting method
title_full The MBS microbial rapid detection system for rapid detection of major pathogenic bacteria in feed: comparison with plate counting method
title_fullStr The MBS microbial rapid detection system for rapid detection of major pathogenic bacteria in feed: comparison with plate counting method
title_full_unstemmed The MBS microbial rapid detection system for rapid detection of major pathogenic bacteria in feed: comparison with plate counting method
title_short The MBS microbial rapid detection system for rapid detection of major pathogenic bacteria in feed: comparison with plate counting method
title_sort mbs microbial rapid detection system for rapid detection of major pathogenic bacteria in feed: comparison with plate counting method
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9541081/
https://www.ncbi.nlm.nih.gov/pubmed/36203160
http://dx.doi.org/10.1186/s12866-022-02655-2
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