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Characterization of costal cartilage allografts
BACKGROUND: Human costal cartilage remains widely used in the reconstruction of soft tissues, particularly within the field of plastic and orthopaedic surgery. The biologic expense of using autologous human costal cartilage has become superseded by the increasingly common use of irradiated costal ca...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons Australia, Ltd
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9541239/ https://www.ncbi.nlm.nih.gov/pubmed/36097422 http://dx.doi.org/10.1111/ans.17967 |
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author | Sinclair, Sonia Walsh, William R. |
author_facet | Sinclair, Sonia Walsh, William R. |
author_sort | Sinclair, Sonia |
collection | PubMed |
description | BACKGROUND: Human costal cartilage remains widely used in the reconstruction of soft tissues, particularly within the field of plastic and orthopaedic surgery. The biologic expense of using autologous human costal cartilage has become superseded by the increasingly common use of irradiated costal cartilage allografts. To date, there has been no histologic investigation of such costal cartilage allografts. This study aims to characterize the histologic variations that exist between different costal cartilage specimens, and to quantify this between specimens in spite of their common anatomical derivation. METHODS: Twenty‐five specimens of cadaveric human costal cartilage were obtained from Australian Biotechnologies. Each specimen was irradiated, sectioned and stained with Haematoxylin and Eosin, Masson's trichrome and tetrachrome stains. After being analysed under light microscopy, specimen dimensions, chondrocyte counts and mineral content was quantified and measured. RESULTS: The median specimen diameter was 8.20 mm, with an interquartile range (IQR) of 1.59 mm. The median measurement from the superficial to basal chondrocyte layer was 1409.91 μm (IQR = 885.59 μm), and the median measurement from superficial to calcified zone was 4146.26 μm (IQR 1441.83 μm). The median chondrocyte area was 442.74 μm(2) (IQR = 2622.72 μm(2)) with their total chondrocyte count ranging from 289 to 591 chondrocytes per square millimetre. The median percentages of collagen and mineral content were 45.17% and 71.82%, respectively (IQR = 20.48%, 14.75%). CONCLUSION: These findings emphasize the histologic and biochemical degree of variation that exists between specimens of human cadaveric costal cartilage on a microscopic level. This has the potential to influence the selection of costal cartilage allografts for reconstructive purposes. |
format | Online Article Text |
id | pubmed-9541239 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley & Sons Australia, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-95412392022-10-14 Characterization of costal cartilage allografts Sinclair, Sonia Walsh, William R. ANZ J Surg Plastic and Reconstructive Surgery BACKGROUND: Human costal cartilage remains widely used in the reconstruction of soft tissues, particularly within the field of plastic and orthopaedic surgery. The biologic expense of using autologous human costal cartilage has become superseded by the increasingly common use of irradiated costal cartilage allografts. To date, there has been no histologic investigation of such costal cartilage allografts. This study aims to characterize the histologic variations that exist between different costal cartilage specimens, and to quantify this between specimens in spite of their common anatomical derivation. METHODS: Twenty‐five specimens of cadaveric human costal cartilage were obtained from Australian Biotechnologies. Each specimen was irradiated, sectioned and stained with Haematoxylin and Eosin, Masson's trichrome and tetrachrome stains. After being analysed under light microscopy, specimen dimensions, chondrocyte counts and mineral content was quantified and measured. RESULTS: The median specimen diameter was 8.20 mm, with an interquartile range (IQR) of 1.59 mm. The median measurement from the superficial to basal chondrocyte layer was 1409.91 μm (IQR = 885.59 μm), and the median measurement from superficial to calcified zone was 4146.26 μm (IQR 1441.83 μm). The median chondrocyte area was 442.74 μm(2) (IQR = 2622.72 μm(2)) with their total chondrocyte count ranging from 289 to 591 chondrocytes per square millimetre. The median percentages of collagen and mineral content were 45.17% and 71.82%, respectively (IQR = 20.48%, 14.75%). CONCLUSION: These findings emphasize the histologic and biochemical degree of variation that exists between specimens of human cadaveric costal cartilage on a microscopic level. This has the potential to influence the selection of costal cartilage allografts for reconstructive purposes. John Wiley & Sons Australia, Ltd 2022-08-09 2022-09 /pmc/articles/PMC9541239/ /pubmed/36097422 http://dx.doi.org/10.1111/ans.17967 Text en © 2022 The Authors. ANZ Journal of Surgery published by John Wiley & Sons Australia, Ltd on behalf of Royal Australasian College of Surgeons. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Plastic and Reconstructive Surgery Sinclair, Sonia Walsh, William R. Characterization of costal cartilage allografts |
title | Characterization of costal cartilage allografts |
title_full | Characterization of costal cartilage allografts |
title_fullStr | Characterization of costal cartilage allografts |
title_full_unstemmed | Characterization of costal cartilage allografts |
title_short | Characterization of costal cartilage allografts |
title_sort | characterization of costal cartilage allografts |
topic | Plastic and Reconstructive Surgery |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9541239/ https://www.ncbi.nlm.nih.gov/pubmed/36097422 http://dx.doi.org/10.1111/ans.17967 |
work_keys_str_mv | AT sinclairsonia characterizationofcostalcartilageallografts AT walshwilliamr characterizationofcostalcartilageallografts |