An optimized platform for efficient siRNA delivery into human NK cells

The molecular networks that regulate natural killer (NK) cell functions are not completely understood. Here, we present a workflow for efficient delivery of siRNA into human NK cells without compromising viability. This methodology represents a promising approach for rapidly interrogating gene funct...

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Detalles Bibliográficos
Autores principales: Palacios, Daniel, Momayyezi, Pouria, Huhn, Oisín, Ask, Eivind Heggernes, Dunst, Josefine, Malmberg, Karl‐Johan, Hammer, Quirin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Technical Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9541389/
https://www.ncbi.nlm.nih.gov/pubmed/35416292
http://dx.doi.org/10.1002/eji.202149710
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author Palacios, Daniel
Momayyezi, Pouria
Huhn, Oisín
Ask, Eivind Heggernes
Dunst, Josefine
Malmberg, Karl‐Johan
Hammer, Quirin
author_facet Palacios, Daniel
Momayyezi, Pouria
Huhn, Oisín
Ask, Eivind Heggernes
Dunst, Josefine
Malmberg, Karl‐Johan
Hammer, Quirin
author_sort Palacios, Daniel
collection PubMed
description The molecular networks that regulate natural killer (NK) cell functions are not completely understood. Here, we present a workflow for efficient delivery of siRNA into human NK cells without compromising viability. This methodology represents a promising approach for rapidly interrogating gene functions in primary human NK cells. [Image: see text]
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spelling pubmed-95413892022-10-14 An optimized platform for efficient siRNA delivery into human NK cells Palacios, Daniel Momayyezi, Pouria Huhn, Oisín Ask, Eivind Heggernes Dunst, Josefine Malmberg, Karl‐Johan Hammer, Quirin Eur J Immunol Technical Report The molecular networks that regulate natural killer (NK) cell functions are not completely understood. Here, we present a workflow for efficient delivery of siRNA into human NK cells without compromising viability. This methodology represents a promising approach for rapidly interrogating gene functions in primary human NK cells. [Image: see text] John Wiley and Sons Inc. 2022-04-22 2022-07 /pmc/articles/PMC9541389/ /pubmed/35416292 http://dx.doi.org/10.1002/eji.202149710 Text en © 2022 The Authors. European Journal of Immunology published by Wiley‐VCH GmbH. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Technical Report
Palacios, Daniel
Momayyezi, Pouria
Huhn, Oisín
Ask, Eivind Heggernes
Dunst, Josefine
Malmberg, Karl‐Johan
Hammer, Quirin
An optimized platform for efficient siRNA delivery into human NK cells
title An optimized platform for efficient siRNA delivery into human NK cells
title_full An optimized platform for efficient siRNA delivery into human NK cells
title_fullStr An optimized platform for efficient siRNA delivery into human NK cells
title_full_unstemmed An optimized platform for efficient siRNA delivery into human NK cells
title_short An optimized platform for efficient siRNA delivery into human NK cells
title_sort optimized platform for efficient sirna delivery into human nk cells
topic Technical Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9541389/
https://www.ncbi.nlm.nih.gov/pubmed/35416292
http://dx.doi.org/10.1002/eji.202149710
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