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In vitro effects of aqueous extract of unfermented rooibos on human spermatozoa
The inability to conceive is a baleful experience for thousands of couples worldwide. Among different well‐known reproductive techniques, medicinal plants have been utilized to treat male infertility. Medicinal plants, provide a therapeutic alternative, which is available and affordable for infertil...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9541412/ https://www.ncbi.nlm.nih.gov/pubmed/35545422 http://dx.doi.org/10.1111/and.14452 |
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author | Takalani, Ndivhuho Beauty Adefolaju, Gbenga Anthony Henkel, Ralf R. Opuwari, Chinyerum Sylvia |
author_facet | Takalani, Ndivhuho Beauty Adefolaju, Gbenga Anthony Henkel, Ralf R. Opuwari, Chinyerum Sylvia |
author_sort | Takalani, Ndivhuho Beauty |
collection | PubMed |
description | The inability to conceive is a baleful experience for thousands of couples worldwide. Among different well‐known reproductive techniques, medicinal plants have been utilized to treat male infertility. Medicinal plants, provide a therapeutic alternative, which is available and affordable for infertile couples. We investigated the direct effect of unfermented rooibos aqueous extract on human spermatozoa. Semen samples (n = 50) collected from donors and patients consulting for fertility were reassigned as normal (n = 22) and abnormal (n = 28) samples based on the outcome of the baseline semen analysis, using the World Health Organization (WHO) cut off value. Semen samples were allowed to liquefy and subsequently washed with human tubular fluid in bovine serum albumin medium. The samples were then treated with aqueous extracts of unfermented rooibos (0, 0.15, 1.5, 15, 150 μg/ml) at 37°C for 1 h and assessed thereafter. Sperm motility, vitality, DNA fragmentation, intracellular reactive oxygen species and mitochondrial membrane potential in both groups remained unchanged (p > 0.05). However, aqueous extract of unfermented rooibos (only at 1.5 μg/ml) significantly increased capacitation and acrosome reaction in the abnormal sample group (p > 0.05). Unfermented rooibos aqueous extract had no deleterious impact on human spermatozoa's function and might be attributed to its antioxidant properties. |
format | Online Article Text |
id | pubmed-9541412 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95414122022-10-14 In vitro effects of aqueous extract of unfermented rooibos on human spermatozoa Takalani, Ndivhuho Beauty Adefolaju, Gbenga Anthony Henkel, Ralf R. Opuwari, Chinyerum Sylvia Andrologia Original Articles The inability to conceive is a baleful experience for thousands of couples worldwide. Among different well‐known reproductive techniques, medicinal plants have been utilized to treat male infertility. Medicinal plants, provide a therapeutic alternative, which is available and affordable for infertile couples. We investigated the direct effect of unfermented rooibos aqueous extract on human spermatozoa. Semen samples (n = 50) collected from donors and patients consulting for fertility were reassigned as normal (n = 22) and abnormal (n = 28) samples based on the outcome of the baseline semen analysis, using the World Health Organization (WHO) cut off value. Semen samples were allowed to liquefy and subsequently washed with human tubular fluid in bovine serum albumin medium. The samples were then treated with aqueous extracts of unfermented rooibos (0, 0.15, 1.5, 15, 150 μg/ml) at 37°C for 1 h and assessed thereafter. Sperm motility, vitality, DNA fragmentation, intracellular reactive oxygen species and mitochondrial membrane potential in both groups remained unchanged (p > 0.05). However, aqueous extract of unfermented rooibos (only at 1.5 μg/ml) significantly increased capacitation and acrosome reaction in the abnormal sample group (p > 0.05). Unfermented rooibos aqueous extract had no deleterious impact on human spermatozoa's function and might be attributed to its antioxidant properties. John Wiley and Sons Inc. 2022-05-11 2022-09 /pmc/articles/PMC9541412/ /pubmed/35545422 http://dx.doi.org/10.1111/and.14452 Text en © 2022 The Authors. Andrologia published by Wiley‐VCH GmbH. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Takalani, Ndivhuho Beauty Adefolaju, Gbenga Anthony Henkel, Ralf R. Opuwari, Chinyerum Sylvia In vitro effects of aqueous extract of unfermented rooibos on human spermatozoa |
title | In vitro effects of aqueous extract of unfermented rooibos on human spermatozoa |
title_full | In vitro effects of aqueous extract of unfermented rooibos on human spermatozoa |
title_fullStr | In vitro effects of aqueous extract of unfermented rooibos on human spermatozoa |
title_full_unstemmed | In vitro effects of aqueous extract of unfermented rooibos on human spermatozoa |
title_short | In vitro effects of aqueous extract of unfermented rooibos on human spermatozoa |
title_sort | in vitro effects of aqueous extract of unfermented rooibos on human spermatozoa |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9541412/ https://www.ncbi.nlm.nih.gov/pubmed/35545422 http://dx.doi.org/10.1111/and.14452 |
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