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A scalable assay for chemical preference of small freshwater fish

Sensing the chemical world is of primary importance for aquatic organisms, and small freshwater fish are increasingly used in toxicology, ethology, and neuroscience by virtue of their ease of manipulation, tissue imaging amenability, and genetic tractability. However, precise behavioral analyses are...

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Autores principales: Gallois, Benjamin, Pontani, Lea-Laetita, Debrégeas, Georges, Candelier, Raphaël
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9541871/
https://www.ncbi.nlm.nih.gov/pubmed/36212190
http://dx.doi.org/10.3389/fnbeh.2022.990792
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author Gallois, Benjamin
Pontani, Lea-Laetita
Debrégeas, Georges
Candelier, Raphaël
author_facet Gallois, Benjamin
Pontani, Lea-Laetita
Debrégeas, Georges
Candelier, Raphaël
author_sort Gallois, Benjamin
collection PubMed
description Sensing the chemical world is of primary importance for aquatic organisms, and small freshwater fish are increasingly used in toxicology, ethology, and neuroscience by virtue of their ease of manipulation, tissue imaging amenability, and genetic tractability. However, precise behavioral analyses are generally challenging to perform due to the lack of knowledge of what chemical the fish are exposed to at any given moment. Here we developed a behavioral assay and a specific infrared dye to probe the preference of young zebrafish for virtually any compound. We found that the innate aversion of zebrafish to citric acid is not mediated by modulation of the swim but rather by immediate avoidance reactions when the product is sensed and that the preference of juvenile zebrafish for ATP changes from repulsion to attraction during successive exposures. We propose an information-based behavioral model for which an exploration index emerges as a relevant behavioral descriptor, complementary to the standard preference index. Our setup features a high versatility in protocols and is automatic and scalable, which paves the way for high-throughput preference compound screening at different ages.
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spelling pubmed-95418712022-10-08 A scalable assay for chemical preference of small freshwater fish Gallois, Benjamin Pontani, Lea-Laetita Debrégeas, Georges Candelier, Raphaël Front Behav Neurosci Behavioral Neuroscience Sensing the chemical world is of primary importance for aquatic organisms, and small freshwater fish are increasingly used in toxicology, ethology, and neuroscience by virtue of their ease of manipulation, tissue imaging amenability, and genetic tractability. However, precise behavioral analyses are generally challenging to perform due to the lack of knowledge of what chemical the fish are exposed to at any given moment. Here we developed a behavioral assay and a specific infrared dye to probe the preference of young zebrafish for virtually any compound. We found that the innate aversion of zebrafish to citric acid is not mediated by modulation of the swim but rather by immediate avoidance reactions when the product is sensed and that the preference of juvenile zebrafish for ATP changes from repulsion to attraction during successive exposures. We propose an information-based behavioral model for which an exploration index emerges as a relevant behavioral descriptor, complementary to the standard preference index. Our setup features a high versatility in protocols and is automatic and scalable, which paves the way for high-throughput preference compound screening at different ages. Frontiers Media S.A. 2022-09-23 /pmc/articles/PMC9541871/ /pubmed/36212190 http://dx.doi.org/10.3389/fnbeh.2022.990792 Text en Copyright © 2022 Gallois, Pontani, Debrégeas and Candelier. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Behavioral Neuroscience
Gallois, Benjamin
Pontani, Lea-Laetita
Debrégeas, Georges
Candelier, Raphaël
A scalable assay for chemical preference of small freshwater fish
title A scalable assay for chemical preference of small freshwater fish
title_full A scalable assay for chemical preference of small freshwater fish
title_fullStr A scalable assay for chemical preference of small freshwater fish
title_full_unstemmed A scalable assay for chemical preference of small freshwater fish
title_short A scalable assay for chemical preference of small freshwater fish
title_sort scalable assay for chemical preference of small freshwater fish
topic Behavioral Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9541871/
https://www.ncbi.nlm.nih.gov/pubmed/36212190
http://dx.doi.org/10.3389/fnbeh.2022.990792
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