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Multi-component pharmacokinetic study of prunus mume fructus extract after oral administration in rats using UPLC-MS/MS
Prunus mume fructus (MF) is used in traditional Chinese medicine and food, as it exerts pharmacological effects, such as antibacterial, antioxidant, antitumour, thirst-relieving, and antidiarrheal effects. In the present study, a reliable and sensitive ultra-high performance liquid chromatography/ta...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9541882/ https://www.ncbi.nlm.nih.gov/pubmed/36210842 http://dx.doi.org/10.3389/fphar.2022.954692 |
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author | Zhu, Yameng Wei, Shujie Cao, Xiunan Wang, Songrui Chang, Yanxu Ouyang, Huizi He, Jun |
author_facet | Zhu, Yameng Wei, Shujie Cao, Xiunan Wang, Songrui Chang, Yanxu Ouyang, Huizi He, Jun |
author_sort | Zhu, Yameng |
collection | PubMed |
description | Prunus mume fructus (MF) is used in traditional Chinese medicine and food, as it exerts pharmacological effects, such as antibacterial, antioxidant, antitumour, thirst-relieving, and antidiarrheal effects. In the present study, a reliable and sensitive ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the simultaneous determination of 16 prototype components (L-(-)-malic acid, 3,4-dihydroxybenzaldehyde, protocatechuic acid, vanillic acid, caffeic acid, D-(-)-quinic acid, citric acid, ferulic acid, syringic acid, cryptochlorogenic acid, neochlorogenic acid, chlorogenic acid, amygdalin, maslinic acid, corosolic acid, and rutin) in rat plasma after oral administration of the MF extract. Plasma samples were prepared via protein precipitation using acetonitrile. The 16 components were separated on an ACQUITY UPLC BEH C18 column (2.1 × 100 mm, 1.7 μm) with a gradient mobile phase system of methanol and 0.1% (v/v) formic acid aqueous solution at a flow rate of 0.3 ml/min. All components were quantitated using Agilent Jet Stream electrospray ionisation in negative ion mode. The intra-day and inter-day accuracies ranged from-9.4 to 9.4%, and the precision of the analytes was less than 14.8%. The extraction recovery rate of the analytes ranged from 63.59 to 109.44% and the matrix effects ranged from 49.25 to 109.28%. Stability studies proved that the analytes were stable under the tested conditions, with a relative standard deviation lower than 13.7%. Hence, the developed method was successfully applied to evaluate the pharmacokinetics of 16 components in the MF extract after oral administration in rats using UPLC-MS/MS. |
format | Online Article Text |
id | pubmed-9541882 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95418822022-10-08 Multi-component pharmacokinetic study of prunus mume fructus extract after oral administration in rats using UPLC-MS/MS Zhu, Yameng Wei, Shujie Cao, Xiunan Wang, Songrui Chang, Yanxu Ouyang, Huizi He, Jun Front Pharmacol Pharmacology Prunus mume fructus (MF) is used in traditional Chinese medicine and food, as it exerts pharmacological effects, such as antibacterial, antioxidant, antitumour, thirst-relieving, and antidiarrheal effects. In the present study, a reliable and sensitive ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the simultaneous determination of 16 prototype components (L-(-)-malic acid, 3,4-dihydroxybenzaldehyde, protocatechuic acid, vanillic acid, caffeic acid, D-(-)-quinic acid, citric acid, ferulic acid, syringic acid, cryptochlorogenic acid, neochlorogenic acid, chlorogenic acid, amygdalin, maslinic acid, corosolic acid, and rutin) in rat plasma after oral administration of the MF extract. Plasma samples were prepared via protein precipitation using acetonitrile. The 16 components were separated on an ACQUITY UPLC BEH C18 column (2.1 × 100 mm, 1.7 μm) with a gradient mobile phase system of methanol and 0.1% (v/v) formic acid aqueous solution at a flow rate of 0.3 ml/min. All components were quantitated using Agilent Jet Stream electrospray ionisation in negative ion mode. The intra-day and inter-day accuracies ranged from-9.4 to 9.4%, and the precision of the analytes was less than 14.8%. The extraction recovery rate of the analytes ranged from 63.59 to 109.44% and the matrix effects ranged from 49.25 to 109.28%. Stability studies proved that the analytes were stable under the tested conditions, with a relative standard deviation lower than 13.7%. Hence, the developed method was successfully applied to evaluate the pharmacokinetics of 16 components in the MF extract after oral administration in rats using UPLC-MS/MS. Frontiers Media S.A. 2022-09-23 /pmc/articles/PMC9541882/ /pubmed/36210842 http://dx.doi.org/10.3389/fphar.2022.954692 Text en Copyright © 2022 Zhu, Wei, Cao, Wang, Chang, Ouyang and He. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Zhu, Yameng Wei, Shujie Cao, Xiunan Wang, Songrui Chang, Yanxu Ouyang, Huizi He, Jun Multi-component pharmacokinetic study of prunus mume fructus extract after oral administration in rats using UPLC-MS/MS |
title | Multi-component pharmacokinetic study of prunus mume fructus extract after oral administration in rats using UPLC-MS/MS |
title_full | Multi-component pharmacokinetic study of prunus mume fructus extract after oral administration in rats using UPLC-MS/MS |
title_fullStr | Multi-component pharmacokinetic study of prunus mume fructus extract after oral administration in rats using UPLC-MS/MS |
title_full_unstemmed | Multi-component pharmacokinetic study of prunus mume fructus extract after oral administration in rats using UPLC-MS/MS |
title_short | Multi-component pharmacokinetic study of prunus mume fructus extract after oral administration in rats using UPLC-MS/MS |
title_sort | multi-component pharmacokinetic study of prunus mume fructus extract after oral administration in rats using uplc-ms/ms |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9541882/ https://www.ncbi.nlm.nih.gov/pubmed/36210842 http://dx.doi.org/10.3389/fphar.2022.954692 |
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