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Selective autophagy and Golgi quality control in Drosophila

The LIR motif-docking site (LDS) of Atg8/LC3 proteins is essential for the binding of LC3-interacting region (LIR)-containing proteins and their subsequent degradation by macroautophagy/autophagy. In our recent study, we created a mutated LDS site in Atg8a, the Drosophila homolog of Atg8/LC3 and fou...

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Autores principales: Gohel, Raksha, Rahman, Ashrafur, Lőrincz, Peter, Nagy, Anikó, Csordás, Gábor, Zhang, Yan, Juhász, Gábor, Nezis, Ioannis P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9542326/
https://www.ncbi.nlm.nih.gov/pubmed/35820026
http://dx.doi.org/10.1080/15548627.2022.2098765
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author Gohel, Raksha
Rahman, Ashrafur
Lőrincz, Peter
Nagy, Anikó
Csordás, Gábor
Zhang, Yan
Juhász, Gábor
Nezis, Ioannis P.
author_facet Gohel, Raksha
Rahman, Ashrafur
Lőrincz, Peter
Nagy, Anikó
Csordás, Gábor
Zhang, Yan
Juhász, Gábor
Nezis, Ioannis P.
author_sort Gohel, Raksha
collection PubMed
description The LIR motif-docking site (LDS) of Atg8/LC3 proteins is essential for the binding of LC3-interacting region (LIR)-containing proteins and their subsequent degradation by macroautophagy/autophagy. In our recent study, we created a mutated LDS site in Atg8a, the Drosophila homolog of Atg8/LC3 and found that LDS mutants accumulate known autophagy substrates and have reduced lifespan. We also conducted quantitative proteomics analyses and identified several proteins that are enriched in the LDS mutants, including Gmap (Golgi microtubule-associated protein). Gmap contains a LIR motif and accumulates in LDS mutants. We showed that Gmap and Atg8a interact in a LIR-LDS dependent manner and that the Golgi size and morphology are altered in Atg8a-LDS and Gmap-LIR motif mutants. Our findings highlight a role for Gmap in the regulation of Golgiphagy.
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spelling pubmed-95423262022-10-08 Selective autophagy and Golgi quality control in Drosophila Gohel, Raksha Rahman, Ashrafur Lőrincz, Peter Nagy, Anikó Csordás, Gábor Zhang, Yan Juhász, Gábor Nezis, Ioannis P. Autophagy Autophagic Punctum The LIR motif-docking site (LDS) of Atg8/LC3 proteins is essential for the binding of LC3-interacting region (LIR)-containing proteins and their subsequent degradation by macroautophagy/autophagy. In our recent study, we created a mutated LDS site in Atg8a, the Drosophila homolog of Atg8/LC3 and found that LDS mutants accumulate known autophagy substrates and have reduced lifespan. We also conducted quantitative proteomics analyses and identified several proteins that are enriched in the LDS mutants, including Gmap (Golgi microtubule-associated protein). Gmap contains a LIR motif and accumulates in LDS mutants. We showed that Gmap and Atg8a interact in a LIR-LDS dependent manner and that the Golgi size and morphology are altered in Atg8a-LDS and Gmap-LIR motif mutants. Our findings highlight a role for Gmap in the regulation of Golgiphagy. Taylor & Francis 2022-07-12 /pmc/articles/PMC9542326/ /pubmed/35820026 http://dx.doi.org/10.1080/15548627.2022.2098765 Text en © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Autophagic Punctum
Gohel, Raksha
Rahman, Ashrafur
Lőrincz, Peter
Nagy, Anikó
Csordás, Gábor
Zhang, Yan
Juhász, Gábor
Nezis, Ioannis P.
Selective autophagy and Golgi quality control in Drosophila
title Selective autophagy and Golgi quality control in Drosophila
title_full Selective autophagy and Golgi quality control in Drosophila
title_fullStr Selective autophagy and Golgi quality control in Drosophila
title_full_unstemmed Selective autophagy and Golgi quality control in Drosophila
title_short Selective autophagy and Golgi quality control in Drosophila
title_sort selective autophagy and golgi quality control in drosophila
topic Autophagic Punctum
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9542326/
https://www.ncbi.nlm.nih.gov/pubmed/35820026
http://dx.doi.org/10.1080/15548627.2022.2098765
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