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Cellular expression of epigenetic markers and oxidative stress in periodontitis lesions of smokers and non‐smokers

OBJECTIVE: To evaluate differences in the cellular expression of epigenetic markers and oxidative stress in periodontitis lesions between current smokers and non‐smokers. BACKGROUND: Tobacco smoking is recognized as one of the major risk factors for periodontitis. However, the mechanisms by which sm...

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Autores principales: Dionigi, Carlotta, Larsson, Lena, Difloe‐Geisert, Julia C., Zitzmann, Nicola U., Berglundh, Tord
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9542336/
https://www.ncbi.nlm.nih.gov/pubmed/35766184
http://dx.doi.org/10.1111/jre.13030
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author Dionigi, Carlotta
Larsson, Lena
Difloe‐Geisert, Julia C.
Zitzmann, Nicola U.
Berglundh, Tord
author_facet Dionigi, Carlotta
Larsson, Lena
Difloe‐Geisert, Julia C.
Zitzmann, Nicola U.
Berglundh, Tord
author_sort Dionigi, Carlotta
collection PubMed
description OBJECTIVE: To evaluate differences in the cellular expression of epigenetic markers and oxidative stress in periodontitis lesions between current smokers and non‐smokers. BACKGROUND: Tobacco smoking is recognized as one of the major risk factors for periodontitis. However, the mechanisms by which smoking affects the progression of the disease remain to be determined. METHODS: Twenty‐five current smokers and 21 non‐smokers with generalized severe periodontitis were included. From each patient, one soft tissue biopsy from a periodontitis site was harvested and prepared for histological analysis. The infiltrated connective tissue (ICT) was selected as the region of interest to assess the cellular expression of epigenetic markers and reactive oxygen/nitrogen species (RONS) by immunohistochemistry. RESULTS: Although the ICT of smokers and non‐smokers did not differ in size or in the expression of markers for DNA damage or oxidative stress, current smokers presented with significantly lower area proportions and densities of cells positive for the epigenetic markers DNMT1 and AcH3. In addition, periodontitis lesions in current smokers presented with a diminished antimicrobial activity, as indicated by significantly lower densities and area proportions of NOX2‐ and iNOS‐positive cells. CONCLUSIONS: Components of the host response and epigenetic mechanisms in periodontitis lesions in smokers are downregulated as opposed to lesions of non‐smokers.
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spelling pubmed-95423362022-10-14 Cellular expression of epigenetic markers and oxidative stress in periodontitis lesions of smokers and non‐smokers Dionigi, Carlotta Larsson, Lena Difloe‐Geisert, Julia C. Zitzmann, Nicola U. Berglundh, Tord J Periodontal Res Original Articles OBJECTIVE: To evaluate differences in the cellular expression of epigenetic markers and oxidative stress in periodontitis lesions between current smokers and non‐smokers. BACKGROUND: Tobacco smoking is recognized as one of the major risk factors for periodontitis. However, the mechanisms by which smoking affects the progression of the disease remain to be determined. METHODS: Twenty‐five current smokers and 21 non‐smokers with generalized severe periodontitis were included. From each patient, one soft tissue biopsy from a periodontitis site was harvested and prepared for histological analysis. The infiltrated connective tissue (ICT) was selected as the region of interest to assess the cellular expression of epigenetic markers and reactive oxygen/nitrogen species (RONS) by immunohistochemistry. RESULTS: Although the ICT of smokers and non‐smokers did not differ in size or in the expression of markers for DNA damage or oxidative stress, current smokers presented with significantly lower area proportions and densities of cells positive for the epigenetic markers DNMT1 and AcH3. In addition, periodontitis lesions in current smokers presented with a diminished antimicrobial activity, as indicated by significantly lower densities and area proportions of NOX2‐ and iNOS‐positive cells. CONCLUSIONS: Components of the host response and epigenetic mechanisms in periodontitis lesions in smokers are downregulated as opposed to lesions of non‐smokers. John Wiley and Sons Inc. 2022-06-29 2022-10 /pmc/articles/PMC9542336/ /pubmed/35766184 http://dx.doi.org/10.1111/jre.13030 Text en © 2022 The Authors. Journal of Periodontal Research published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Dionigi, Carlotta
Larsson, Lena
Difloe‐Geisert, Julia C.
Zitzmann, Nicola U.
Berglundh, Tord
Cellular expression of epigenetic markers and oxidative stress in periodontitis lesions of smokers and non‐smokers
title Cellular expression of epigenetic markers and oxidative stress in periodontitis lesions of smokers and non‐smokers
title_full Cellular expression of epigenetic markers and oxidative stress in periodontitis lesions of smokers and non‐smokers
title_fullStr Cellular expression of epigenetic markers and oxidative stress in periodontitis lesions of smokers and non‐smokers
title_full_unstemmed Cellular expression of epigenetic markers and oxidative stress in periodontitis lesions of smokers and non‐smokers
title_short Cellular expression of epigenetic markers and oxidative stress in periodontitis lesions of smokers and non‐smokers
title_sort cellular expression of epigenetic markers and oxidative stress in periodontitis lesions of smokers and non‐smokers
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9542336/
https://www.ncbi.nlm.nih.gov/pubmed/35766184
http://dx.doi.org/10.1111/jre.13030
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