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A phasor‐based approach to improve optical sectioning in any confocal microscope with a tunable pinhole
Confocal fluorescence microscopy is a well‐established imaging technique capable of generating thin optical sections of biological specimens. Optical sectioning in confocal microscopy is mainly determined by the size of the pinhole, a small aperture placed in front of a point detector. In principle,...
Autores principales: | D'Amico, Morgana, Di Franco, Elisabetta, Cerutti, Elena, Barresi, Vincenza, Condorelli, Daniele, Diaspro, Alberto, Lanzanò, Luca |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9542401/ https://www.ncbi.nlm.nih.gov/pubmed/35686877 http://dx.doi.org/10.1002/jemt.24178 |
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