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A Field‐Independent Method for the Rapid Generation of Hyperpolarized [1‐(13)C]Pyruvate in Clean Water Solutions for Biomedical Applications

Hyperpolarization methods in magnetic resonance enhance the signals by several orders of magnitude, opening new windows for real‐time investigations of dynamic processes in vitro and in vivo. Here, we propose a field‐independent para‐hydrogen‐based pulsed method to produce rapidly hyperpolarized (13...

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Autores principales: Mamone, Salvatore, Jagtap, Anil P., Korchak, Sergey, Ding, Yonghong, Sternkopf, Sonja, Glöggler, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9543135/
https://www.ncbi.nlm.nih.gov/pubmed/35723041
http://dx.doi.org/10.1002/anie.202206298
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author Mamone, Salvatore
Jagtap, Anil P.
Korchak, Sergey
Ding, Yonghong
Sternkopf, Sonja
Glöggler, Stefan
author_facet Mamone, Salvatore
Jagtap, Anil P.
Korchak, Sergey
Ding, Yonghong
Sternkopf, Sonja
Glöggler, Stefan
author_sort Mamone, Salvatore
collection PubMed
description Hyperpolarization methods in magnetic resonance enhance the signals by several orders of magnitude, opening new windows for real‐time investigations of dynamic processes in vitro and in vivo. Here, we propose a field‐independent para‐hydrogen‐based pulsed method to produce rapidly hyperpolarized (13)C‐labeled substrates. We demonstrate the method by polarizing the carboxylic carbon of the pyruvate moiety in a purposely designed precursor to 24 % at ≈22 mT. Following a fast purification procedure, we measure 8 % polarization on free [1‐(13)C]pyruvate in clean water solutions at physiological conditions at 7 T. The enhanced signals allow real‐time monitoring of the pyruvate‐lactate conversion in cancer cells, demonstrating the potential of the method for biomedical applications in combination with existing or developing magnetic resonance technologies.
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spelling pubmed-95431352022-10-14 A Field‐Independent Method for the Rapid Generation of Hyperpolarized [1‐(13)C]Pyruvate in Clean Water Solutions for Biomedical Applications Mamone, Salvatore Jagtap, Anil P. Korchak, Sergey Ding, Yonghong Sternkopf, Sonja Glöggler, Stefan Angew Chem Int Ed Engl Communications Hyperpolarization methods in magnetic resonance enhance the signals by several orders of magnitude, opening new windows for real‐time investigations of dynamic processes in vitro and in vivo. Here, we propose a field‐independent para‐hydrogen‐based pulsed method to produce rapidly hyperpolarized (13)C‐labeled substrates. We demonstrate the method by polarizing the carboxylic carbon of the pyruvate moiety in a purposely designed precursor to 24 % at ≈22 mT. Following a fast purification procedure, we measure 8 % polarization on free [1‐(13)C]pyruvate in clean water solutions at physiological conditions at 7 T. The enhanced signals allow real‐time monitoring of the pyruvate‐lactate conversion in cancer cells, demonstrating the potential of the method for biomedical applications in combination with existing or developing magnetic resonance technologies. John Wiley and Sons Inc. 2022-07-14 2022-08-22 /pmc/articles/PMC9543135/ /pubmed/35723041 http://dx.doi.org/10.1002/anie.202206298 Text en © 2022 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Communications
Mamone, Salvatore
Jagtap, Anil P.
Korchak, Sergey
Ding, Yonghong
Sternkopf, Sonja
Glöggler, Stefan
A Field‐Independent Method for the Rapid Generation of Hyperpolarized [1‐(13)C]Pyruvate in Clean Water Solutions for Biomedical Applications
title A Field‐Independent Method for the Rapid Generation of Hyperpolarized [1‐(13)C]Pyruvate in Clean Water Solutions for Biomedical Applications
title_full A Field‐Independent Method for the Rapid Generation of Hyperpolarized [1‐(13)C]Pyruvate in Clean Water Solutions for Biomedical Applications
title_fullStr A Field‐Independent Method for the Rapid Generation of Hyperpolarized [1‐(13)C]Pyruvate in Clean Water Solutions for Biomedical Applications
title_full_unstemmed A Field‐Independent Method for the Rapid Generation of Hyperpolarized [1‐(13)C]Pyruvate in Clean Water Solutions for Biomedical Applications
title_short A Field‐Independent Method for the Rapid Generation of Hyperpolarized [1‐(13)C]Pyruvate in Clean Water Solutions for Biomedical Applications
title_sort field‐independent method for the rapid generation of hyperpolarized [1‐(13)c]pyruvate in clean water solutions for biomedical applications
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9543135/
https://www.ncbi.nlm.nih.gov/pubmed/35723041
http://dx.doi.org/10.1002/anie.202206298
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