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Ino80 is required for H2A.Z eviction from hypha‐specific promoters and hyphal development of Candida albicans

ATP‐dependent chromatin remodeling complexes play important roles in many essential cellular processes, including transcription regulation, DNA replication, and repair. Evicting H2A.Z, a variant of histone H2A, from the promoter of hypha‐specific genes is required for hyphal formation in Candida alb...

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Detalles Bibliográficos
Autores principales: Zhao, Qun, Dai, Baodi, Wu, Hongyu, Zhu, Wencheng, Chen, Jiangye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9543228/
https://www.ncbi.nlm.nih.gov/pubmed/35713098
http://dx.doi.org/10.1111/mmi.14954
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author Zhao, Qun
Dai, Baodi
Wu, Hongyu
Zhu, Wencheng
Chen, Jiangye
author_facet Zhao, Qun
Dai, Baodi
Wu, Hongyu
Zhu, Wencheng
Chen, Jiangye
author_sort Zhao, Qun
collection PubMed
description ATP‐dependent chromatin remodeling complexes play important roles in many essential cellular processes, including transcription regulation, DNA replication, and repair. Evicting H2A.Z, a variant of histone H2A, from the promoter of hypha‐specific genes is required for hyphal formation in Candida albicans. However, the mechanism that regulates H2A.Z removal during hyphal formation remains unknown. In this study, we demonstrated that Ino80, the core catalytic subunit of the INO80 complex, was recruited to hypha‐specific promoters during hyphal induction in Arp8 dependent manner and facilitated the removal of H2A.Z. Deleting INO80 or mutating the ATPase site of Ino80 impairs the expression of hypha‐specific genes (HSGs) and hyphal development. In addition, we showed that Ino80 was essential for the virulence of C. albicans during systemic infections in mice. Interestingly, Arp5, an INO80 complex‐specific component, acts in concert with Ino80 during DNA damage responses but is dispensable for hyphal induction. Our findings clarified that Ino80 was critical for hyphal development, DNA damage response, and pathogenesis in C. albicans.
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spelling pubmed-95432282022-10-14 Ino80 is required for H2A.Z eviction from hypha‐specific promoters and hyphal development of Candida albicans Zhao, Qun Dai, Baodi Wu, Hongyu Zhu, Wencheng Chen, Jiangye Mol Microbiol Research Articles ATP‐dependent chromatin remodeling complexes play important roles in many essential cellular processes, including transcription regulation, DNA replication, and repair. Evicting H2A.Z, a variant of histone H2A, from the promoter of hypha‐specific genes is required for hyphal formation in Candida albicans. However, the mechanism that regulates H2A.Z removal during hyphal formation remains unknown. In this study, we demonstrated that Ino80, the core catalytic subunit of the INO80 complex, was recruited to hypha‐specific promoters during hyphal induction in Arp8 dependent manner and facilitated the removal of H2A.Z. Deleting INO80 or mutating the ATPase site of Ino80 impairs the expression of hypha‐specific genes (HSGs) and hyphal development. In addition, we showed that Ino80 was essential for the virulence of C. albicans during systemic infections in mice. Interestingly, Arp5, an INO80 complex‐specific component, acts in concert with Ino80 during DNA damage responses but is dispensable for hyphal induction. Our findings clarified that Ino80 was critical for hyphal development, DNA damage response, and pathogenesis in C. albicans. John Wiley and Sons Inc. 2022-06-28 2022 /pmc/articles/PMC9543228/ /pubmed/35713098 http://dx.doi.org/10.1111/mmi.14954 Text en © 2022 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Zhao, Qun
Dai, Baodi
Wu, Hongyu
Zhu, Wencheng
Chen, Jiangye
Ino80 is required for H2A.Z eviction from hypha‐specific promoters and hyphal development of Candida albicans
title Ino80 is required for H2A.Z eviction from hypha‐specific promoters and hyphal development of Candida albicans
title_full Ino80 is required for H2A.Z eviction from hypha‐specific promoters and hyphal development of Candida albicans
title_fullStr Ino80 is required for H2A.Z eviction from hypha‐specific promoters and hyphal development of Candida albicans
title_full_unstemmed Ino80 is required for H2A.Z eviction from hypha‐specific promoters and hyphal development of Candida albicans
title_short Ino80 is required for H2A.Z eviction from hypha‐specific promoters and hyphal development of Candida albicans
title_sort ino80 is required for h2a.z eviction from hypha‐specific promoters and hyphal development of candida albicans
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9543228/
https://www.ncbi.nlm.nih.gov/pubmed/35713098
http://dx.doi.org/10.1111/mmi.14954
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