OMIP‐079: Cell cycle of CD4 (+) and CD8 (+) naïve/memory T cell subsets, and of Treg cells from mouse spleen

A multicolor flow cytometry panel was designed and optimized to define the following nine mouse T cell subsets: Treg (CD3(+) CD4(+) CD8(−) FoxP3(+)), CD4(+) T naïve (CD3(+) CD4(+) CD8(−)FoxP3(−) CD44(int/low) CD62L(+)), CD4(+) T central memory (CD3(+) CD4(+) CD8(−) FoxP3(−) CD44(high) CD62L(+)), CD4...

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Detalles Bibliográficos
Autores principales: Natalini, Ambra, Simonetti, Sonia, Favaretto, Gabriele, Peruzzi, Giovanna, Antonangeli, Fabrizio, Santoni, Angela, Muñoz‐Ruiz, Miguel, Hayday, Adrian, Di Rosa, Francesca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2021
Materias:
Omip
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9543383/
https://www.ncbi.nlm.nih.gov/pubmed/34668313
http://dx.doi.org/10.1002/cyto.a.24509
Descripción
Sumario:A multicolor flow cytometry panel was designed and optimized to define the following nine mouse T cell subsets: Treg (CD3(+) CD4(+) CD8(−) FoxP3(+)), CD4(+) T naïve (CD3(+) CD4(+) CD8(−)FoxP3(−) CD44(int/low) CD62L(+)), CD4(+) T central memory (CD3(+) CD4(+) CD8(−) FoxP3(−) CD44(high) CD62L(+)), CD4(+) T effector memory (CD3(+) CD4(+) CD8(−) FoxP3(−) CD44(high) CD62L(−)), CD4(+) T EMRA (CD3(+) CD4(+) CD8(−) FoxP3(−) CD44(int/low) CD62L(−)), CD8(+) T naïve (CD3(+) CD8(+) CD4(−) CD44(int/low) CD62L(+)), CD8(+) T central memory (CD3(+) CD8(+) CD4(−) CD44(high) CD62L(+)), CD8(+) T effector memory (CD3(+) CD8(+) CD4(−) CD44(high) CD62L(−)), and CD8(+) T EMRA (CD3(+) CD8(+) CD4(−) CD44(int/low) CD62L(−)). In each T cell subset, a dual staining for Ki‐67 expression and DNA content was employed to distinguish the following cell cycle phases: G(0) (Ki67(−), with 2n DNA), G(1) (Ki67(+), with 2n DNA), and S‐G(2)/M (Ki67(+), with 2n < DNA ≤ 4n). This panel was established for the analysis of mouse (C57BL/6J) spleen.

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