Cargando…
OMIP‐079: Cell cycle of CD4 (+) and CD8 (+) naïve/memory T cell subsets, and of Treg cells from mouse spleen
A multicolor flow cytometry panel was designed and optimized to define the following nine mouse T cell subsets: Treg (CD3(+) CD4(+) CD8(−) FoxP3(+)), CD4(+) T naïve (CD3(+) CD4(+) CD8(−)FoxP3(−) CD44(int/low) CD62L(+)), CD4(+) T central memory (CD3(+) CD4(+) CD8(−) FoxP3(−) CD44(high) CD62L(+)), CD4...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley & Sons, Inc.
2021
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9543383/ https://www.ncbi.nlm.nih.gov/pubmed/34668313 http://dx.doi.org/10.1002/cyto.a.24509 |
| _version_ | 1784804360937537536 |
|---|---|
| author | Natalini, Ambra Simonetti, Sonia Favaretto, Gabriele Peruzzi, Giovanna Antonangeli, Fabrizio Santoni, Angela Muñoz‐Ruiz, Miguel Hayday, Adrian Di Rosa, Francesca |
| author_facet | Natalini, Ambra Simonetti, Sonia Favaretto, Gabriele Peruzzi, Giovanna Antonangeli, Fabrizio Santoni, Angela Muñoz‐Ruiz, Miguel Hayday, Adrian Di Rosa, Francesca |
| author_sort | Natalini, Ambra |
| collection | PubMed |
| description | A multicolor flow cytometry panel was designed and optimized to define the following nine mouse T cell subsets: Treg (CD3(+) CD4(+) CD8(−) FoxP3(+)), CD4(+) T naïve (CD3(+) CD4(+) CD8(−)FoxP3(−) CD44(int/low) CD62L(+)), CD4(+) T central memory (CD3(+) CD4(+) CD8(−) FoxP3(−) CD44(high) CD62L(+)), CD4(+) T effector memory (CD3(+) CD4(+) CD8(−) FoxP3(−) CD44(high) CD62L(−)), CD4(+) T EMRA (CD3(+) CD4(+) CD8(−) FoxP3(−) CD44(int/low) CD62L(−)), CD8(+) T naïve (CD3(+) CD8(+) CD4(−) CD44(int/low) CD62L(+)), CD8(+) T central memory (CD3(+) CD8(+) CD4(−) CD44(high) CD62L(+)), CD8(+) T effector memory (CD3(+) CD8(+) CD4(−) CD44(high) CD62L(−)), and CD8(+) T EMRA (CD3(+) CD8(+) CD4(−) CD44(int/low) CD62L(−)). In each T cell subset, a dual staining for Ki‐67 expression and DNA content was employed to distinguish the following cell cycle phases: G(0) (Ki67(−), with 2n DNA), G(1) (Ki67(+), with 2n DNA), and S‐G(2)/M (Ki67(+), with 2n < DNA ≤ 4n). This panel was established for the analysis of mouse (C57BL/6J) spleen. |
| format | Online Article Text |
| id | pubmed-9543383 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | John Wiley & Sons, Inc. |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-95433832022-10-14 OMIP‐079: Cell cycle of CD4 (+) and CD8 (+) naïve/memory T cell subsets, and of Treg cells from mouse spleen Natalini, Ambra Simonetti, Sonia Favaretto, Gabriele Peruzzi, Giovanna Antonangeli, Fabrizio Santoni, Angela Muñoz‐Ruiz, Miguel Hayday, Adrian Di Rosa, Francesca Cytometry A Omip A multicolor flow cytometry panel was designed and optimized to define the following nine mouse T cell subsets: Treg (CD3(+) CD4(+) CD8(−) FoxP3(+)), CD4(+) T naïve (CD3(+) CD4(+) CD8(−)FoxP3(−) CD44(int/low) CD62L(+)), CD4(+) T central memory (CD3(+) CD4(+) CD8(−) FoxP3(−) CD44(high) CD62L(+)), CD4(+) T effector memory (CD3(+) CD4(+) CD8(−) FoxP3(−) CD44(high) CD62L(−)), CD4(+) T EMRA (CD3(+) CD4(+) CD8(−) FoxP3(−) CD44(int/low) CD62L(−)), CD8(+) T naïve (CD3(+) CD8(+) CD4(−) CD44(int/low) CD62L(+)), CD8(+) T central memory (CD3(+) CD8(+) CD4(−) CD44(high) CD62L(+)), CD8(+) T effector memory (CD3(+) CD8(+) CD4(−) CD44(high) CD62L(−)), and CD8(+) T EMRA (CD3(+) CD8(+) CD4(−) CD44(int/low) CD62L(−)). In each T cell subset, a dual staining for Ki‐67 expression and DNA content was employed to distinguish the following cell cycle phases: G(0) (Ki67(−), with 2n DNA), G(1) (Ki67(+), with 2n DNA), and S‐G(2)/M (Ki67(+), with 2n < DNA ≤ 4n). This panel was established for the analysis of mouse (C57BL/6J) spleen. John Wiley & Sons, Inc. 2021-10-19 2021-12 /pmc/articles/PMC9543383/ /pubmed/34668313 http://dx.doi.org/10.1002/cyto.a.24509 Text en © 2021 The Authors. Cytometry Part A published by Wiley Periodicals LLC. on behalf of International Society for Advancement of Cytometry. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
| spellingShingle | Omip Natalini, Ambra Simonetti, Sonia Favaretto, Gabriele Peruzzi, Giovanna Antonangeli, Fabrizio Santoni, Angela Muñoz‐Ruiz, Miguel Hayday, Adrian Di Rosa, Francesca OMIP‐079: Cell cycle of CD4 (+) and CD8 (+) naïve/memory T cell subsets, and of Treg cells from mouse spleen |
| title |
OMIP‐079: Cell cycle of CD4
(+) and CD8
(+) naïve/memory T cell subsets, and of Treg cells from mouse spleen |
| title_full |
OMIP‐079: Cell cycle of CD4
(+) and CD8
(+) naïve/memory T cell subsets, and of Treg cells from mouse spleen |
| title_fullStr |
OMIP‐079: Cell cycle of CD4
(+) and CD8
(+) naïve/memory T cell subsets, and of Treg cells from mouse spleen |
| title_full_unstemmed |
OMIP‐079: Cell cycle of CD4
(+) and CD8
(+) naïve/memory T cell subsets, and of Treg cells from mouse spleen |
| title_short |
OMIP‐079: Cell cycle of CD4
(+) and CD8
(+) naïve/memory T cell subsets, and of Treg cells from mouse spleen |
| title_sort | omip‐079: cell cycle of cd4
(+) and cd8
(+) naïve/memory t cell subsets, and of treg cells from mouse spleen |
| topic | Omip |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9543383/ https://www.ncbi.nlm.nih.gov/pubmed/34668313 http://dx.doi.org/10.1002/cyto.a.24509 |
| work_keys_str_mv | AT nataliniambra omip079cellcycleofcd4andcd8naivememorytcellsubsetsandoftregcellsfrommousespleen AT simonettisonia omip079cellcycleofcd4andcd8naivememorytcellsubsetsandoftregcellsfrommousespleen AT favarettogabriele omip079cellcycleofcd4andcd8naivememorytcellsubsetsandoftregcellsfrommousespleen AT peruzzigiovanna omip079cellcycleofcd4andcd8naivememorytcellsubsetsandoftregcellsfrommousespleen AT antonangelifabrizio omip079cellcycleofcd4andcd8naivememorytcellsubsetsandoftregcellsfrommousespleen AT santoniangela omip079cellcycleofcd4andcd8naivememorytcellsubsetsandoftregcellsfrommousespleen AT munozruizmiguel omip079cellcycleofcd4andcd8naivememorytcellsubsetsandoftregcellsfrommousespleen AT haydayadrian omip079cellcycleofcd4andcd8naivememorytcellsubsetsandoftregcellsfrommousespleen AT dirosafrancesca omip079cellcycleofcd4andcd8naivememorytcellsubsetsandoftregcellsfrommousespleen |