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Zaxinone synthase controls arbuscular mycorrhizal colonization level in rice

The Oryza sativa (rice) carotenoid cleavage dioxygenase OsZAS was described to produce zaxinone, a plant growth‐promoting apocarotenoid. A zas mutant line showed reduced arbuscular mycorrhizal (AM) colonization, but the mechanisms underlying this behavior are unknown. Here, we investigated how OsZAS...

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Autores principales: Votta, Cristina, Fiorilli, Valentina, Haider, Imran, Wang, Jian You, Balestrini, Raffaella, Petřík, Ivan, Tarkowská, Danuše, Novák, Ondřej, Serikbayeva, Akmaral, Bonfante, Paola, Al‐Babili, Salim, Lanfranco, Luisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9543690/
https://www.ncbi.nlm.nih.gov/pubmed/35877598
http://dx.doi.org/10.1111/tpj.15917
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author Votta, Cristina
Fiorilli, Valentina
Haider, Imran
Wang, Jian You
Balestrini, Raffaella
Petřík, Ivan
Tarkowská, Danuše
Novák, Ondřej
Serikbayeva, Akmaral
Bonfante, Paola
Al‐Babili, Salim
Lanfranco, Luisa
author_facet Votta, Cristina
Fiorilli, Valentina
Haider, Imran
Wang, Jian You
Balestrini, Raffaella
Petřík, Ivan
Tarkowská, Danuše
Novák, Ondřej
Serikbayeva, Akmaral
Bonfante, Paola
Al‐Babili, Salim
Lanfranco, Luisa
author_sort Votta, Cristina
collection PubMed
description The Oryza sativa (rice) carotenoid cleavage dioxygenase OsZAS was described to produce zaxinone, a plant growth‐promoting apocarotenoid. A zas mutant line showed reduced arbuscular mycorrhizal (AM) colonization, but the mechanisms underlying this behavior are unknown. Here, we investigated how OsZAS and exogenous zaxinone treatment regulate mycorrhization. Micromolar exogenous supply of zaxinone rescued root growth but not the mycorrhizal defects of the zas mutant, and even reduced mycorrhization in wild‐type and zas genotypes. The zas line did not display the increase in the level of strigolactones (SLs) that was observed in wild‐type plants at 7 days post‐inoculation with AM fungus. Moreover, exogenous treatment with the synthetic SL analog GR24 rescued the zas mutant mycorrhizal phenotype, indicating that the lower AM colonization rate of zas is caused by a deficiency in SLs at the early stages of the interaction, and indicating that during this phase OsZAS activity is required to induce SL production, possibly mediated by the Dwarf14‐Like (D14L) signaling pathway. OsZAS is expressed in arbuscule‐containing cells, and OsPT11prom::OsZAS transgenic lines, where OsZAS expression is driven by the OsPT11 promoter active in arbusculated cells, exhibit increased mycorrhization compared with the wild type. Overall, our results show that the genetic manipulation of OsZAS activity in planta leads to a different effect on AM symbiosis from that of exogenous zaxinone treatment, and demonstrate that OsZAS influences the extent of AM colonization, acting as a component of a regulatory network that involves SLs.
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spelling pubmed-95436902022-10-14 Zaxinone synthase controls arbuscular mycorrhizal colonization level in rice Votta, Cristina Fiorilli, Valentina Haider, Imran Wang, Jian You Balestrini, Raffaella Petřík, Ivan Tarkowská, Danuše Novák, Ondřej Serikbayeva, Akmaral Bonfante, Paola Al‐Babili, Salim Lanfranco, Luisa Plant J Original Articles The Oryza sativa (rice) carotenoid cleavage dioxygenase OsZAS was described to produce zaxinone, a plant growth‐promoting apocarotenoid. A zas mutant line showed reduced arbuscular mycorrhizal (AM) colonization, but the mechanisms underlying this behavior are unknown. Here, we investigated how OsZAS and exogenous zaxinone treatment regulate mycorrhization. Micromolar exogenous supply of zaxinone rescued root growth but not the mycorrhizal defects of the zas mutant, and even reduced mycorrhization in wild‐type and zas genotypes. The zas line did not display the increase in the level of strigolactones (SLs) that was observed in wild‐type plants at 7 days post‐inoculation with AM fungus. Moreover, exogenous treatment with the synthetic SL analog GR24 rescued the zas mutant mycorrhizal phenotype, indicating that the lower AM colonization rate of zas is caused by a deficiency in SLs at the early stages of the interaction, and indicating that during this phase OsZAS activity is required to induce SL production, possibly mediated by the Dwarf14‐Like (D14L) signaling pathway. OsZAS is expressed in arbuscule‐containing cells, and OsPT11prom::OsZAS transgenic lines, where OsZAS expression is driven by the OsPT11 promoter active in arbusculated cells, exhibit increased mycorrhization compared with the wild type. Overall, our results show that the genetic manipulation of OsZAS activity in planta leads to a different effect on AM symbiosis from that of exogenous zaxinone treatment, and demonstrate that OsZAS influences the extent of AM colonization, acting as a component of a regulatory network that involves SLs. John Wiley and Sons Inc. 2022-08-17 2022-09 /pmc/articles/PMC9543690/ /pubmed/35877598 http://dx.doi.org/10.1111/tpj.15917 Text en © 2022 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Votta, Cristina
Fiorilli, Valentina
Haider, Imran
Wang, Jian You
Balestrini, Raffaella
Petřík, Ivan
Tarkowská, Danuše
Novák, Ondřej
Serikbayeva, Akmaral
Bonfante, Paola
Al‐Babili, Salim
Lanfranco, Luisa
Zaxinone synthase controls arbuscular mycorrhizal colonization level in rice
title Zaxinone synthase controls arbuscular mycorrhizal colonization level in rice
title_full Zaxinone synthase controls arbuscular mycorrhizal colonization level in rice
title_fullStr Zaxinone synthase controls arbuscular mycorrhizal colonization level in rice
title_full_unstemmed Zaxinone synthase controls arbuscular mycorrhizal colonization level in rice
title_short Zaxinone synthase controls arbuscular mycorrhizal colonization level in rice
title_sort zaxinone synthase controls arbuscular mycorrhizal colonization level in rice
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9543690/
https://www.ncbi.nlm.nih.gov/pubmed/35877598
http://dx.doi.org/10.1111/tpj.15917
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