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The helper NLR immune protein NRC3 mediates the hypersensitive cell death caused by the cell-surface receptor Cf-4
Cell surface pattern recognition receptors (PRRs) activate immune responses that can include the hypersensitive cell death. However, the pathways that link PRRs to the cell death response are poorly understood. Here, we show that the cell surface receptor-like protein Cf-4 requires the intracellular...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9543701/ https://www.ncbi.nlm.nih.gov/pubmed/36137148 http://dx.doi.org/10.1371/journal.pgen.1010414 |
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author | Kourelis, Jiorgos Contreras, Mauricio P. Harant, Adeline Pai, Hsuan Lüdke, Daniel Adachi, Hiroaki Derevnina, Lida Wu, Chih-Hang Kamoun, Sophien |
author_facet | Kourelis, Jiorgos Contreras, Mauricio P. Harant, Adeline Pai, Hsuan Lüdke, Daniel Adachi, Hiroaki Derevnina, Lida Wu, Chih-Hang Kamoun, Sophien |
author_sort | Kourelis, Jiorgos |
collection | PubMed |
description | Cell surface pattern recognition receptors (PRRs) activate immune responses that can include the hypersensitive cell death. However, the pathways that link PRRs to the cell death response are poorly understood. Here, we show that the cell surface receptor-like protein Cf-4 requires the intracellular nucleotide-binding domain leucine-rich repeat containing receptor (NLR) NRC3 to trigger a confluent cell death response upon detection of the fungal effector Avr4 in leaves of Nicotiana benthamiana. This NRC3 activity requires an intact N-terminal MADA motif, a conserved signature of coiled-coil (CC)-type plant NLRs that is required for resistosome-mediated immune responses. A chimeric protein with the N-terminal α1 helix of Arabidopsis ZAR1 swapped into NRC3 retains the capacity to mediate Cf-4 hypersensitive cell death. Pathogen effectors acting as suppressors of NRC3 can suppress Cf-4-triggered hypersensitive cell-death. Our findings link the NLR resistosome model to the hypersensitive cell death caused by a cell surface PRR. |
format | Online Article Text |
id | pubmed-9543701 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-95437012022-10-08 The helper NLR immune protein NRC3 mediates the hypersensitive cell death caused by the cell-surface receptor Cf-4 Kourelis, Jiorgos Contreras, Mauricio P. Harant, Adeline Pai, Hsuan Lüdke, Daniel Adachi, Hiroaki Derevnina, Lida Wu, Chih-Hang Kamoun, Sophien PLoS Genet Research Article Cell surface pattern recognition receptors (PRRs) activate immune responses that can include the hypersensitive cell death. However, the pathways that link PRRs to the cell death response are poorly understood. Here, we show that the cell surface receptor-like protein Cf-4 requires the intracellular nucleotide-binding domain leucine-rich repeat containing receptor (NLR) NRC3 to trigger a confluent cell death response upon detection of the fungal effector Avr4 in leaves of Nicotiana benthamiana. This NRC3 activity requires an intact N-terminal MADA motif, a conserved signature of coiled-coil (CC)-type plant NLRs that is required for resistosome-mediated immune responses. A chimeric protein with the N-terminal α1 helix of Arabidopsis ZAR1 swapped into NRC3 retains the capacity to mediate Cf-4 hypersensitive cell death. Pathogen effectors acting as suppressors of NRC3 can suppress Cf-4-triggered hypersensitive cell-death. Our findings link the NLR resistosome model to the hypersensitive cell death caused by a cell surface PRR. Public Library of Science 2022-09-22 /pmc/articles/PMC9543701/ /pubmed/36137148 http://dx.doi.org/10.1371/journal.pgen.1010414 Text en © 2022 Kourelis et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Kourelis, Jiorgos Contreras, Mauricio P. Harant, Adeline Pai, Hsuan Lüdke, Daniel Adachi, Hiroaki Derevnina, Lida Wu, Chih-Hang Kamoun, Sophien The helper NLR immune protein NRC3 mediates the hypersensitive cell death caused by the cell-surface receptor Cf-4 |
title | The helper NLR immune protein NRC3 mediates the hypersensitive cell death caused by the cell-surface receptor Cf-4 |
title_full | The helper NLR immune protein NRC3 mediates the hypersensitive cell death caused by the cell-surface receptor Cf-4 |
title_fullStr | The helper NLR immune protein NRC3 mediates the hypersensitive cell death caused by the cell-surface receptor Cf-4 |
title_full_unstemmed | The helper NLR immune protein NRC3 mediates the hypersensitive cell death caused by the cell-surface receptor Cf-4 |
title_short | The helper NLR immune protein NRC3 mediates the hypersensitive cell death caused by the cell-surface receptor Cf-4 |
title_sort | helper nlr immune protein nrc3 mediates the hypersensitive cell death caused by the cell-surface receptor cf-4 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9543701/ https://www.ncbi.nlm.nih.gov/pubmed/36137148 http://dx.doi.org/10.1371/journal.pgen.1010414 |
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