Cargando…
Random amplified polymorphic DNA analysis reveals no clear link between Staphylococcus epidermidis and acute mastitis
Mastitis is commonly experienced by breastfeeding women. While Staphylococcus aureus is usually implicated in infectious mastitis, coagulase‐negative staphylococci (CoNS) are a possible alternative pathogen. This case‐control study examined the role of CoNS in mastitis using isolates cultured from b...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2022
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9543939/ https://www.ncbi.nlm.nih.gov/pubmed/35229883 http://dx.doi.org/10.1111/ajo.13503 |
| _version_ | 1784804488342667264 |
|---|---|
| author | Cullinane, Meabh Scofield, Lauren Murray, Gerald L. Payne, Matthew S. Bennett, Catherine M. Garland, Suzanne M. Amir, Lisa H. |
| author_facet | Cullinane, Meabh Scofield, Lauren Murray, Gerald L. Payne, Matthew S. Bennett, Catherine M. Garland, Suzanne M. Amir, Lisa H. |
| author_sort | Cullinane, Meabh |
| collection | PubMed |
| description | Mastitis is commonly experienced by breastfeeding women. While Staphylococcus aureus is usually implicated in infectious mastitis, coagulase‐negative staphylococci (CoNS) are a possible alternative pathogen. This case‐control study examined the role of CoNS in mastitis using isolates cultured from breast milk of 20 women with mastitis and 16 women without mastitis. Gene sequencing determined bacterial species, and random amplified polymorphic DNA (RAPD) analysis investigated strain‐level variation. The majority of CoNS isolates were Staphylococcus epidermidis (182/199; 91%). RAPD analysis identified 33 unique S. epidermidis profiles, with no specific profile associated with mastitis cases. |
| format | Online Article Text |
| id | pubmed-9543939 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2022 |
| publisher | John Wiley and Sons Inc. |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-95439392022-10-14 Random amplified polymorphic DNA analysis reveals no clear link between Staphylococcus epidermidis and acute mastitis Cullinane, Meabh Scofield, Lauren Murray, Gerald L. Payne, Matthew S. Bennett, Catherine M. Garland, Suzanne M. Amir, Lisa H. Aust N Z J Obstet Gynaecol Short Communications Mastitis is commonly experienced by breastfeeding women. While Staphylococcus aureus is usually implicated in infectious mastitis, coagulase‐negative staphylococci (CoNS) are a possible alternative pathogen. This case‐control study examined the role of CoNS in mastitis using isolates cultured from breast milk of 20 women with mastitis and 16 women without mastitis. Gene sequencing determined bacterial species, and random amplified polymorphic DNA (RAPD) analysis investigated strain‐level variation. The majority of CoNS isolates were Staphylococcus epidermidis (182/199; 91%). RAPD analysis identified 33 unique S. epidermidis profiles, with no specific profile associated with mastitis cases. John Wiley and Sons Inc. 2022-03-01 2022-08 /pmc/articles/PMC9543939/ /pubmed/35229883 http://dx.doi.org/10.1111/ajo.13503 Text en © 2022 The Authors. Australian and New Zealand Journal of Obstetrics and Gynaecology published by John Wiley & Sons Australia, Ltd on behalf of Royal Australian and New Zealand College of Obstetricians and Gynaecologists https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
| spellingShingle | Short Communications Cullinane, Meabh Scofield, Lauren Murray, Gerald L. Payne, Matthew S. Bennett, Catherine M. Garland, Suzanne M. Amir, Lisa H. Random amplified polymorphic DNA analysis reveals no clear link between Staphylococcus epidermidis and acute mastitis |
| title | Random amplified polymorphic DNA analysis reveals no clear link between Staphylococcus epidermidis and acute mastitis |
| title_full | Random amplified polymorphic DNA analysis reveals no clear link between Staphylococcus epidermidis and acute mastitis |
| title_fullStr | Random amplified polymorphic DNA analysis reveals no clear link between Staphylococcus epidermidis and acute mastitis |
| title_full_unstemmed | Random amplified polymorphic DNA analysis reveals no clear link between Staphylococcus epidermidis and acute mastitis |
| title_short | Random amplified polymorphic DNA analysis reveals no clear link between Staphylococcus epidermidis and acute mastitis |
| title_sort | random amplified polymorphic dna analysis reveals no clear link between staphylococcus epidermidis and acute mastitis |
| topic | Short Communications |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9543939/ https://www.ncbi.nlm.nih.gov/pubmed/35229883 http://dx.doi.org/10.1111/ajo.13503 |
| work_keys_str_mv | AT cullinanemeabh randomamplifiedpolymorphicdnaanalysisrevealsnoclearlinkbetweenstaphylococcusepidermidisandacutemastitis AT scofieldlauren randomamplifiedpolymorphicdnaanalysisrevealsnoclearlinkbetweenstaphylococcusepidermidisandacutemastitis AT murraygeraldl randomamplifiedpolymorphicdnaanalysisrevealsnoclearlinkbetweenstaphylococcusepidermidisandacutemastitis AT paynematthews randomamplifiedpolymorphicdnaanalysisrevealsnoclearlinkbetweenstaphylococcusepidermidisandacutemastitis AT bennettcatherinem randomamplifiedpolymorphicdnaanalysisrevealsnoclearlinkbetweenstaphylococcusepidermidisandacutemastitis AT garlandsuzannem randomamplifiedpolymorphicdnaanalysisrevealsnoclearlinkbetweenstaphylococcusepidermidisandacutemastitis AT amirlisah randomamplifiedpolymorphicdnaanalysisrevealsnoclearlinkbetweenstaphylococcusepidermidisandacutemastitis AT randomamplifiedpolymorphicdnaanalysisrevealsnoclearlinkbetweenstaphylococcusepidermidisandacutemastitis |