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FIONA1-mediated methylation of the 3’UTR of FLC affects FLC transcript levels and flowering in Arabidopsis

Adenosine bases of RNA can be transiently modified by the deposition of a methyl-group to form N(6)-methyladenosine (m(6)A). This adenosine-methylation is an ancient process and the enzymes involved are evolutionary highly conserved. A genetic screen designed to identify suppressors of late flowerin...

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Autores principales: Sun, Bin, Bhati, Kaushal Kumar, Song, Peizhe, Edwards, Ashleigh, Petri, Louise, Kruusvee, Valdeko, Blaakmeer, Anko, Dolde, Ulla, Rodrigues, Vandasue, Straub, Daniel, Yang, Junbo, Jia, Guifang, Wenkel, Stephan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9543952/
https://www.ncbi.nlm.nih.gov/pubmed/36166469
http://dx.doi.org/10.1371/journal.pgen.1010386
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author Sun, Bin
Bhati, Kaushal Kumar
Song, Peizhe
Edwards, Ashleigh
Petri, Louise
Kruusvee, Valdeko
Blaakmeer, Anko
Dolde, Ulla
Rodrigues, Vandasue
Straub, Daniel
Yang, Junbo
Jia, Guifang
Wenkel, Stephan
author_facet Sun, Bin
Bhati, Kaushal Kumar
Song, Peizhe
Edwards, Ashleigh
Petri, Louise
Kruusvee, Valdeko
Blaakmeer, Anko
Dolde, Ulla
Rodrigues, Vandasue
Straub, Daniel
Yang, Junbo
Jia, Guifang
Wenkel, Stephan
author_sort Sun, Bin
collection PubMed
description Adenosine bases of RNA can be transiently modified by the deposition of a methyl-group to form N(6)-methyladenosine (m(6)A). This adenosine-methylation is an ancient process and the enzymes involved are evolutionary highly conserved. A genetic screen designed to identify suppressors of late flowering transgenic Arabidopsis plants overexpressing the miP1a microProtein yielded a new allele of the FIONA1 (FIO1) m(6)A-methyltransferase. To characterize the early flowering phenotype of fio1 mutant plants we employed an integrative approach of mRNA-seq, Nanopore direct RNA-sequencing and meRIP-seq to identify differentially expressed transcripts as well as differentially methylated RNAs. We provide evidence that FIO1 is the elusive methyltransferase responsible for the 3’-end methylation of the FLOWERING LOCUS C (FLC) transcript. Furthermore, our genetic and biochemical data suggest that 3’-methylation stabilizes FLC mRNAs and non-methylated FLC is a target for rapid degradation.
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spelling pubmed-95439522022-10-08 FIONA1-mediated methylation of the 3’UTR of FLC affects FLC transcript levels and flowering in Arabidopsis Sun, Bin Bhati, Kaushal Kumar Song, Peizhe Edwards, Ashleigh Petri, Louise Kruusvee, Valdeko Blaakmeer, Anko Dolde, Ulla Rodrigues, Vandasue Straub, Daniel Yang, Junbo Jia, Guifang Wenkel, Stephan PLoS Genet Research Article Adenosine bases of RNA can be transiently modified by the deposition of a methyl-group to form N(6)-methyladenosine (m(6)A). This adenosine-methylation is an ancient process and the enzymes involved are evolutionary highly conserved. A genetic screen designed to identify suppressors of late flowering transgenic Arabidopsis plants overexpressing the miP1a microProtein yielded a new allele of the FIONA1 (FIO1) m(6)A-methyltransferase. To characterize the early flowering phenotype of fio1 mutant plants we employed an integrative approach of mRNA-seq, Nanopore direct RNA-sequencing and meRIP-seq to identify differentially expressed transcripts as well as differentially methylated RNAs. We provide evidence that FIO1 is the elusive methyltransferase responsible for the 3’-end methylation of the FLOWERING LOCUS C (FLC) transcript. Furthermore, our genetic and biochemical data suggest that 3’-methylation stabilizes FLC mRNAs and non-methylated FLC is a target for rapid degradation. Public Library of Science 2022-09-27 /pmc/articles/PMC9543952/ /pubmed/36166469 http://dx.doi.org/10.1371/journal.pgen.1010386 Text en © 2022 Sun et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Sun, Bin
Bhati, Kaushal Kumar
Song, Peizhe
Edwards, Ashleigh
Petri, Louise
Kruusvee, Valdeko
Blaakmeer, Anko
Dolde, Ulla
Rodrigues, Vandasue
Straub, Daniel
Yang, Junbo
Jia, Guifang
Wenkel, Stephan
FIONA1-mediated methylation of the 3’UTR of FLC affects FLC transcript levels and flowering in Arabidopsis
title FIONA1-mediated methylation of the 3’UTR of FLC affects FLC transcript levels and flowering in Arabidopsis
title_full FIONA1-mediated methylation of the 3’UTR of FLC affects FLC transcript levels and flowering in Arabidopsis
title_fullStr FIONA1-mediated methylation of the 3’UTR of FLC affects FLC transcript levels and flowering in Arabidopsis
title_full_unstemmed FIONA1-mediated methylation of the 3’UTR of FLC affects FLC transcript levels and flowering in Arabidopsis
title_short FIONA1-mediated methylation of the 3’UTR of FLC affects FLC transcript levels and flowering in Arabidopsis
title_sort fiona1-mediated methylation of the 3’utr of flc affects flc transcript levels and flowering in arabidopsis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9543952/
https://www.ncbi.nlm.nih.gov/pubmed/36166469
http://dx.doi.org/10.1371/journal.pgen.1010386
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