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Volumetric assessment and longitudinal changes of subcortical structures in formalinized Beagle brains

High field MRI is an advanced technique for diagnostic and research purposes on animal models, such as the Beagle dog. In this context, studies on neuroscience applications, e.g. aging and neuro-pathologies, are currently increasing. This led to a need for reference values, in terms of volumetric as...

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Autores principales: Del Signore, Francesca, Arribarat, Germain, Della Salda, Leonardo, Mogicato, Giovanni, Deviers, Alexandra, Cartiaux, Benjamin, Vignoli, Massimo, Peran, Patrice, de Pasquale, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9543981/
https://www.ncbi.nlm.nih.gov/pubmed/36206292
http://dx.doi.org/10.1371/journal.pone.0261484
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author Del Signore, Francesca
Arribarat, Germain
Della Salda, Leonardo
Mogicato, Giovanni
Deviers, Alexandra
Cartiaux, Benjamin
Vignoli, Massimo
Peran, Patrice
de Pasquale, Francesco
author_facet Del Signore, Francesca
Arribarat, Germain
Della Salda, Leonardo
Mogicato, Giovanni
Deviers, Alexandra
Cartiaux, Benjamin
Vignoli, Massimo
Peran, Patrice
de Pasquale, Francesco
author_sort Del Signore, Francesca
collection PubMed
description High field MRI is an advanced technique for diagnostic and research purposes on animal models, such as the Beagle dog. In this context, studies on neuroscience applications, e.g. aging and neuro-pathologies, are currently increasing. This led to a need for reference values, in terms of volumetric assessment, for the structures typically involved. Nowadays, several canine brain MRI atlases have been provided. However, no reports are available regarding the measurements’ reproducibility and little is known about the effect of formalin on MRI segmentation. Here, we assessed the segmentation variability of selected structures among operators (two operators segmented the same data) in a sample of 11 Beagle dogs. Then, we analyzed, for one Beagle dog, the longitudinal volumetric changes of these structures. We considered four conditions: in vivo, post mortem (after euthanasia), ex vivo (brain extracted and studied after 1 month in formalin, and after 12 months). The MRI data were collected with a 3 T scanner. Our findings suggest that the segmentation procedure was overall reproducible since only slight statistical differences were detected. In the post mortem/ ex vivo comparison, most structures showed a higher contrast, thereby leading to greater reproducibility between operators. We observed a net increase in the volume of the studied structures. This could be justified by the intrinsic relaxation time changes observed because of the formalin fixation. This led to an improvement in brain structure visualization and segmentation. To conclude, MRI-based segmentation seems to be a useful and accurate tool that allows longitudinal studies on formalin-fixed brains.
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spelling pubmed-95439812022-10-08 Volumetric assessment and longitudinal changes of subcortical structures in formalinized Beagle brains Del Signore, Francesca Arribarat, Germain Della Salda, Leonardo Mogicato, Giovanni Deviers, Alexandra Cartiaux, Benjamin Vignoli, Massimo Peran, Patrice de Pasquale, Francesco PLoS One Research Article High field MRI is an advanced technique for diagnostic and research purposes on animal models, such as the Beagle dog. In this context, studies on neuroscience applications, e.g. aging and neuro-pathologies, are currently increasing. This led to a need for reference values, in terms of volumetric assessment, for the structures typically involved. Nowadays, several canine brain MRI atlases have been provided. However, no reports are available regarding the measurements’ reproducibility and little is known about the effect of formalin on MRI segmentation. Here, we assessed the segmentation variability of selected structures among operators (two operators segmented the same data) in a sample of 11 Beagle dogs. Then, we analyzed, for one Beagle dog, the longitudinal volumetric changes of these structures. We considered four conditions: in vivo, post mortem (after euthanasia), ex vivo (brain extracted and studied after 1 month in formalin, and after 12 months). The MRI data were collected with a 3 T scanner. Our findings suggest that the segmentation procedure was overall reproducible since only slight statistical differences were detected. In the post mortem/ ex vivo comparison, most structures showed a higher contrast, thereby leading to greater reproducibility between operators. We observed a net increase in the volume of the studied structures. This could be justified by the intrinsic relaxation time changes observed because of the formalin fixation. This led to an improvement in brain structure visualization and segmentation. To conclude, MRI-based segmentation seems to be a useful and accurate tool that allows longitudinal studies on formalin-fixed brains. Public Library of Science 2022-10-07 /pmc/articles/PMC9543981/ /pubmed/36206292 http://dx.doi.org/10.1371/journal.pone.0261484 Text en © 2022 Del Signore et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Del Signore, Francesca
Arribarat, Germain
Della Salda, Leonardo
Mogicato, Giovanni
Deviers, Alexandra
Cartiaux, Benjamin
Vignoli, Massimo
Peran, Patrice
de Pasquale, Francesco
Volumetric assessment and longitudinal changes of subcortical structures in formalinized Beagle brains
title Volumetric assessment and longitudinal changes of subcortical structures in formalinized Beagle brains
title_full Volumetric assessment and longitudinal changes of subcortical structures in formalinized Beagle brains
title_fullStr Volumetric assessment and longitudinal changes of subcortical structures in formalinized Beagle brains
title_full_unstemmed Volumetric assessment and longitudinal changes of subcortical structures in formalinized Beagle brains
title_short Volumetric assessment and longitudinal changes of subcortical structures in formalinized Beagle brains
title_sort volumetric assessment and longitudinal changes of subcortical structures in formalinized beagle brains
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9543981/
https://www.ncbi.nlm.nih.gov/pubmed/36206292
http://dx.doi.org/10.1371/journal.pone.0261484
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