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Natural and induced variations in transcriptional regulator genes result in low‐nicotine phenotypes in tobacco
In tobacco, the homologous ETHYLENE RESPONSE FACTOR (ERF) transcription factors ERF199 and ERF189 coordinate the transcription of multiple metabolic genes involved in nicotine biosynthesis. Natural alleles at the NIC1 and NIC2 loci greatly affect alkaloid accumulation and overlap with ERF199 and ERF...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9544004/ https://www.ncbi.nlm.nih.gov/pubmed/35883194 http://dx.doi.org/10.1111/tpj.15923 |
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author | Shoji, Tsubasa Moriyama, Koki Sierro, Nicolas Ouadi, Sonia Ivanov, Nikolai V. Hashimoto, Takashi Saito, Kazuki |
author_facet | Shoji, Tsubasa Moriyama, Koki Sierro, Nicolas Ouadi, Sonia Ivanov, Nikolai V. Hashimoto, Takashi Saito, Kazuki |
author_sort | Shoji, Tsubasa |
collection | PubMed |
description | In tobacco, the homologous ETHYLENE RESPONSE FACTOR (ERF) transcription factors ERF199 and ERF189 coordinate the transcription of multiple metabolic genes involved in nicotine biosynthesis. Natural alleles at the NIC1 and NIC2 loci greatly affect alkaloid accumulation and overlap with ERF199 and ERF189 in the tobacco genome, respectively. In this study, we identified several low‐nicotine tobacco varieties lacking ERF199 or ERF189 from a tobacco germplasm collection. We characterized the sequence of these new nic1 and nic2 alleles, as well as the previously defined alleles nic1‐1 and nic2‐1. Moreover, we examined the influence of different nic alleles on alkaloid contents and expression levels of genes related to nicotine biosynthesis. We also demonstrated that the deletion of a distal genomic region attenuates ERF199 expression, resulting in a moderately negative effect on the alkaloid phenotype. Our study provides new insights into the regulation of nicotine biosynthesis and novel genetic resources to breed low‐nicotine tobacco. |
format | Online Article Text |
id | pubmed-9544004 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95440042022-10-14 Natural and induced variations in transcriptional regulator genes result in low‐nicotine phenotypes in tobacco Shoji, Tsubasa Moriyama, Koki Sierro, Nicolas Ouadi, Sonia Ivanov, Nikolai V. Hashimoto, Takashi Saito, Kazuki Plant J Original Articles In tobacco, the homologous ETHYLENE RESPONSE FACTOR (ERF) transcription factors ERF199 and ERF189 coordinate the transcription of multiple metabolic genes involved in nicotine biosynthesis. Natural alleles at the NIC1 and NIC2 loci greatly affect alkaloid accumulation and overlap with ERF199 and ERF189 in the tobacco genome, respectively. In this study, we identified several low‐nicotine tobacco varieties lacking ERF199 or ERF189 from a tobacco germplasm collection. We characterized the sequence of these new nic1 and nic2 alleles, as well as the previously defined alleles nic1‐1 and nic2‐1. Moreover, we examined the influence of different nic alleles on alkaloid contents and expression levels of genes related to nicotine biosynthesis. We also demonstrated that the deletion of a distal genomic region attenuates ERF199 expression, resulting in a moderately negative effect on the alkaloid phenotype. Our study provides new insights into the regulation of nicotine biosynthesis and novel genetic resources to breed low‐nicotine tobacco. John Wiley and Sons Inc. 2022-08-11 2022-09 /pmc/articles/PMC9544004/ /pubmed/35883194 http://dx.doi.org/10.1111/tpj.15923 Text en © 2022 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Shoji, Tsubasa Moriyama, Koki Sierro, Nicolas Ouadi, Sonia Ivanov, Nikolai V. Hashimoto, Takashi Saito, Kazuki Natural and induced variations in transcriptional regulator genes result in low‐nicotine phenotypes in tobacco |
title | Natural and induced variations in transcriptional regulator genes result in low‐nicotine phenotypes in tobacco |
title_full | Natural and induced variations in transcriptional regulator genes result in low‐nicotine phenotypes in tobacco |
title_fullStr | Natural and induced variations in transcriptional regulator genes result in low‐nicotine phenotypes in tobacco |
title_full_unstemmed | Natural and induced variations in transcriptional regulator genes result in low‐nicotine phenotypes in tobacco |
title_short | Natural and induced variations in transcriptional regulator genes result in low‐nicotine phenotypes in tobacco |
title_sort | natural and induced variations in transcriptional regulator genes result in low‐nicotine phenotypes in tobacco |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9544004/ https://www.ncbi.nlm.nih.gov/pubmed/35883194 http://dx.doi.org/10.1111/tpj.15923 |
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