Cargando…

A single LC‐MS/MS validated method for tulathromycin quantification in plasma, seminal plasma, and urine to be applied in a pharmacokinetic study in bull

Tulathromycin is a macrolide antibiotic generally used for the treatment of respiratory diseases in cattle and swine. This work proposes an improvement of a previously published LC‐MS/MS method for tulathromycin determination in pig serum, here validated in three different bull matrices: plasma, sem...

Descripción completa

Detalles Bibliográficos
Autores principales: Barbarossa, Andrea, Bardhi, Anisa, Gazzotti, Teresa, Mari, Gaetano, Pagliuca, Giampiero
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9544005/
https://www.ncbi.nlm.nih.gov/pubmed/35385608
http://dx.doi.org/10.1002/dta.3270
_version_ 1784804503898292224
author Barbarossa, Andrea
Bardhi, Anisa
Gazzotti, Teresa
Mari, Gaetano
Pagliuca, Giampiero
author_facet Barbarossa, Andrea
Bardhi, Anisa
Gazzotti, Teresa
Mari, Gaetano
Pagliuca, Giampiero
author_sort Barbarossa, Andrea
collection PubMed
description Tulathromycin is a macrolide antibiotic generally used for the treatment of respiratory diseases in cattle and swine. This work proposes an improvement of a previously published LC‐MS/MS method for tulathromycin determination in pig serum, here validated in three different bull matrices: plasma, seminal plasma, and urine. The approach is based on a quick protein precipitation with acetonitrile, filtration, and sample dilution before injection, allowing to rapidly process large batches of samples. Analytes separation was obtained using a BEH C18 (50 × 2.1 mm, 1.7 μm) column, maintained at 40°C with a chromatographic run of 5 min. The method was fully validated over concentration ranges suitable for field levels of tulathromycin found in each matrix (0.01–1 μg/ml for plasma, 0.05–5 μg/ml for seminal plasma, and 0.1–10 μg/ml for urine), showing good linearity during each day of testing (R (2) always >0.99). Accuracy and precision were within ±15% at all QC concentrations in all the three matrices. Furthermore, the use of tulathromycine‐d7 as internal standard mitigated the potential impacts of matrix effect. The validated technique was successfully applied to samples collected during a pharmacokinetic study in bulls, allowing to monitor tulathromycin concentrations over time in the three matrices. To our knowledge, this is the first validated approach for LC‐MS/MS quantification of tulathromycin in seminal plasma and urine.
format Online
Article
Text
id pubmed-9544005
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-95440052022-10-14 A single LC‐MS/MS validated method for tulathromycin quantification in plasma, seminal plasma, and urine to be applied in a pharmacokinetic study in bull Barbarossa, Andrea Bardhi, Anisa Gazzotti, Teresa Mari, Gaetano Pagliuca, Giampiero Drug Test Anal Short Communications Tulathromycin is a macrolide antibiotic generally used for the treatment of respiratory diseases in cattle and swine. This work proposes an improvement of a previously published LC‐MS/MS method for tulathromycin determination in pig serum, here validated in three different bull matrices: plasma, seminal plasma, and urine. The approach is based on a quick protein precipitation with acetonitrile, filtration, and sample dilution before injection, allowing to rapidly process large batches of samples. Analytes separation was obtained using a BEH C18 (50 × 2.1 mm, 1.7 μm) column, maintained at 40°C with a chromatographic run of 5 min. The method was fully validated over concentration ranges suitable for field levels of tulathromycin found in each matrix (0.01–1 μg/ml for plasma, 0.05–5 μg/ml for seminal plasma, and 0.1–10 μg/ml for urine), showing good linearity during each day of testing (R (2) always >0.99). Accuracy and precision were within ±15% at all QC concentrations in all the three matrices. Furthermore, the use of tulathromycine‐d7 as internal standard mitigated the potential impacts of matrix effect. The validated technique was successfully applied to samples collected during a pharmacokinetic study in bulls, allowing to monitor tulathromycin concentrations over time in the three matrices. To our knowledge, this is the first validated approach for LC‐MS/MS quantification of tulathromycin in seminal plasma and urine. John Wiley and Sons Inc. 2022-04-12 2022-08 /pmc/articles/PMC9544005/ /pubmed/35385608 http://dx.doi.org/10.1002/dta.3270 Text en © 2022 The Authors. Drug Testing and Analysis published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Short Communications
Barbarossa, Andrea
Bardhi, Anisa
Gazzotti, Teresa
Mari, Gaetano
Pagliuca, Giampiero
A single LC‐MS/MS validated method for tulathromycin quantification in plasma, seminal plasma, and urine to be applied in a pharmacokinetic study in bull
title A single LC‐MS/MS validated method for tulathromycin quantification in plasma, seminal plasma, and urine to be applied in a pharmacokinetic study in bull
title_full A single LC‐MS/MS validated method for tulathromycin quantification in plasma, seminal plasma, and urine to be applied in a pharmacokinetic study in bull
title_fullStr A single LC‐MS/MS validated method for tulathromycin quantification in plasma, seminal plasma, and urine to be applied in a pharmacokinetic study in bull
title_full_unstemmed A single LC‐MS/MS validated method for tulathromycin quantification in plasma, seminal plasma, and urine to be applied in a pharmacokinetic study in bull
title_short A single LC‐MS/MS validated method for tulathromycin quantification in plasma, seminal plasma, and urine to be applied in a pharmacokinetic study in bull
title_sort single lc‐ms/ms validated method for tulathromycin quantification in plasma, seminal plasma, and urine to be applied in a pharmacokinetic study in bull
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9544005/
https://www.ncbi.nlm.nih.gov/pubmed/35385608
http://dx.doi.org/10.1002/dta.3270
work_keys_str_mv AT barbarossaandrea asinglelcmsmsvalidatedmethodfortulathromycinquantificationinplasmaseminalplasmaandurinetobeappliedinapharmacokineticstudyinbull
AT bardhianisa asinglelcmsmsvalidatedmethodfortulathromycinquantificationinplasmaseminalplasmaandurinetobeappliedinapharmacokineticstudyinbull
AT gazzottiteresa asinglelcmsmsvalidatedmethodfortulathromycinquantificationinplasmaseminalplasmaandurinetobeappliedinapharmacokineticstudyinbull
AT marigaetano asinglelcmsmsvalidatedmethodfortulathromycinquantificationinplasmaseminalplasmaandurinetobeappliedinapharmacokineticstudyinbull
AT pagliucagiampiero asinglelcmsmsvalidatedmethodfortulathromycinquantificationinplasmaseminalplasmaandurinetobeappliedinapharmacokineticstudyinbull
AT barbarossaandrea singlelcmsmsvalidatedmethodfortulathromycinquantificationinplasmaseminalplasmaandurinetobeappliedinapharmacokineticstudyinbull
AT bardhianisa singlelcmsmsvalidatedmethodfortulathromycinquantificationinplasmaseminalplasmaandurinetobeappliedinapharmacokineticstudyinbull
AT gazzottiteresa singlelcmsmsvalidatedmethodfortulathromycinquantificationinplasmaseminalplasmaandurinetobeappliedinapharmacokineticstudyinbull
AT marigaetano singlelcmsmsvalidatedmethodfortulathromycinquantificationinplasmaseminalplasmaandurinetobeappliedinapharmacokineticstudyinbull
AT pagliucagiampiero singlelcmsmsvalidatedmethodfortulathromycinquantificationinplasmaseminalplasmaandurinetobeappliedinapharmacokineticstudyinbull