Assessment of the frequency of Mycobacterium bovis shedding in the faeces of naturally and experimentally TB infected cattle

AIMS: To assess the prevalence of Mycobacterium bovis bacilli in faecal samples of tuberculous cattle, and to better understand the risk of environmental dissemination of bovine tuberculosis (TB) through the spreading of manure or slurry. METHODS AND RESULTS: Faecal samples were collected from 72 na...

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Autores principales: Palmer, Si, Williams, Gareth A., Brady, Colm, Ryan, Eoin, Malczewska, Karolina, Bull, Tim J., Hogarth, Philip J., Sawyer, Jason
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9544641/
https://www.ncbi.nlm.nih.gov/pubmed/35729710
http://dx.doi.org/10.1111/jam.15677
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author Palmer, Si
Williams, Gareth A.
Brady, Colm
Ryan, Eoin
Malczewska, Karolina
Bull, Tim J.
Hogarth, Philip J.
Sawyer, Jason
author_facet Palmer, Si
Williams, Gareth A.
Brady, Colm
Ryan, Eoin
Malczewska, Karolina
Bull, Tim J.
Hogarth, Philip J.
Sawyer, Jason
author_sort Palmer, Si
collection PubMed
description AIMS: To assess the prevalence of Mycobacterium bovis bacilli in faecal samples of tuberculous cattle, and to better understand the risk of environmental dissemination of bovine tuberculosis (TB) through the spreading of manure or slurry. METHODS AND RESULTS: Faecal samples were collected from 72 naturally infected cattle with visible lesions of TB that had reacted to the tuberculin skin test and 12 cattle experimentally infected with M. bovis. These were examined by microbial culture and PCR to assess the presence of M. bovis bacilli. There were no positive cultures from any naturally infected test reactor animal. A single M. bovis colony was cultured from a faecal sample from one of the experimentally infected animals. A single PCR positive result was obtained from the faecal sample of one naturally infected test reactor. CONCLUSIONS: The prevalence of M. bovis in the faecal samples of TB‐infected cattle was extremely low. SIGNIFICANCE AND IMPACT OF THE STUDY: The results suggest that the risk of spreading TB through the use of slurry or manure as an agricultural fertilizer is lower than that suggested in some historical literature. The results could inform a reconsideration of current risk assessments and guidelines on the disposal of manure and slurry from TB‐infected herds.
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spelling pubmed-95446412022-10-14 Assessment of the frequency of Mycobacterium bovis shedding in the faeces of naturally and experimentally TB infected cattle Palmer, Si Williams, Gareth A. Brady, Colm Ryan, Eoin Malczewska, Karolina Bull, Tim J. Hogarth, Philip J. Sawyer, Jason J Appl Microbiol Original Articles AIMS: To assess the prevalence of Mycobacterium bovis bacilli in faecal samples of tuberculous cattle, and to better understand the risk of environmental dissemination of bovine tuberculosis (TB) through the spreading of manure or slurry. METHODS AND RESULTS: Faecal samples were collected from 72 naturally infected cattle with visible lesions of TB that had reacted to the tuberculin skin test and 12 cattle experimentally infected with M. bovis. These were examined by microbial culture and PCR to assess the presence of M. bovis bacilli. There were no positive cultures from any naturally infected test reactor animal. A single M. bovis colony was cultured from a faecal sample from one of the experimentally infected animals. A single PCR positive result was obtained from the faecal sample of one naturally infected test reactor. CONCLUSIONS: The prevalence of M. bovis in the faecal samples of TB‐infected cattle was extremely low. SIGNIFICANCE AND IMPACT OF THE STUDY: The results suggest that the risk of spreading TB through the use of slurry or manure as an agricultural fertilizer is lower than that suggested in some historical literature. The results could inform a reconsideration of current risk assessments and guidelines on the disposal of manure and slurry from TB‐infected herds. John Wiley and Sons Inc. 2022-07-08 2022-09 /pmc/articles/PMC9544641/ /pubmed/35729710 http://dx.doi.org/10.1111/jam.15677 Text en © 2022 Crown copyright. Journal of Applied Microbiology published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology. This article is published with the permission of the Controller of HMSO and the Queen's Printer for Scotland. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Palmer, Si
Williams, Gareth A.
Brady, Colm
Ryan, Eoin
Malczewska, Karolina
Bull, Tim J.
Hogarth, Philip J.
Sawyer, Jason
Assessment of the frequency of Mycobacterium bovis shedding in the faeces of naturally and experimentally TB infected cattle
title Assessment of the frequency of Mycobacterium bovis shedding in the faeces of naturally and experimentally TB infected cattle
title_full Assessment of the frequency of Mycobacterium bovis shedding in the faeces of naturally and experimentally TB infected cattle
title_fullStr Assessment of the frequency of Mycobacterium bovis shedding in the faeces of naturally and experimentally TB infected cattle
title_full_unstemmed Assessment of the frequency of Mycobacterium bovis shedding in the faeces of naturally and experimentally TB infected cattle
title_short Assessment of the frequency of Mycobacterium bovis shedding in the faeces of naturally and experimentally TB infected cattle
title_sort assessment of the frequency of mycobacterium bovis shedding in the faeces of naturally and experimentally tb infected cattle
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9544641/
https://www.ncbi.nlm.nih.gov/pubmed/35729710
http://dx.doi.org/10.1111/jam.15677
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