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Escherichia coli robustly expresses ATP synthase at growth rate‐maximizing concentrations

Fitness‐enhancing adaptations of protein expression and its regulation are an important aspect of bacterial evolution. A key question is whether evolution has led to optimal protein expression that maximizes immediate growth rate (short‐term fitness) in a robust manner (consistently across diverse c...

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Autores principales: Rabbers, Iraes, Bruggeman, Frank J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9544884/
https://www.ncbi.nlm.nih.gov/pubmed/35175666
http://dx.doi.org/10.1111/febs.16401
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author Rabbers, Iraes
Bruggeman, Frank J.
author_facet Rabbers, Iraes
Bruggeman, Frank J.
author_sort Rabbers, Iraes
collection PubMed
description Fitness‐enhancing adaptations of protein expression and its regulation are an important aspect of bacterial evolution. A key question is whether evolution has led to optimal protein expression that maximizes immediate growth rate (short‐term fitness) in a robust manner (consistently across diverse conditions). Alternatively, they could display suboptimal short‐term fitness, because they cannot do better or because they instead strive for long‐term fitness maximization by, for instance, preparing for future conditions. To address this question, we focus on the ATP‐producing enzyme F(1)F(0) H(+)‐ATPase, which is an abundant enzyme and ubiquitously expressed across conditions. Its expression is highly regulated and dependent on growth rate and nutrient conditions. For instance, during growth on sugars, when metabolism is overflowing acetate, glycolysis supplies most ATP, while H(+)‐ATPase is the main source of ATP synthesis during growth on acetate. We tested the optimality of H(+)‐ATPase expression in Escherichia coli across different nutrient conditions. In all tested conditions, wild‐type E. coli expresses its H(+)‐ATPase remarkably close (within a few per cent) to optimal concentrations that maximize immediate growth rate. This work indicates that bacteria can indeed achieve robust optimal protein expression for immediate growth‐rate maximization.
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spelling pubmed-95448842022-10-14 Escherichia coli robustly expresses ATP synthase at growth rate‐maximizing concentrations Rabbers, Iraes Bruggeman, Frank J. FEBS J Original Articles Fitness‐enhancing adaptations of protein expression and its regulation are an important aspect of bacterial evolution. A key question is whether evolution has led to optimal protein expression that maximizes immediate growth rate (short‐term fitness) in a robust manner (consistently across diverse conditions). Alternatively, they could display suboptimal short‐term fitness, because they cannot do better or because they instead strive for long‐term fitness maximization by, for instance, preparing for future conditions. To address this question, we focus on the ATP‐producing enzyme F(1)F(0) H(+)‐ATPase, which is an abundant enzyme and ubiquitously expressed across conditions. Its expression is highly regulated and dependent on growth rate and nutrient conditions. For instance, during growth on sugars, when metabolism is overflowing acetate, glycolysis supplies most ATP, while H(+)‐ATPase is the main source of ATP synthesis during growth on acetate. We tested the optimality of H(+)‐ATPase expression in Escherichia coli across different nutrient conditions. In all tested conditions, wild‐type E. coli expresses its H(+)‐ATPase remarkably close (within a few per cent) to optimal concentrations that maximize immediate growth rate. This work indicates that bacteria can indeed achieve robust optimal protein expression for immediate growth‐rate maximization. John Wiley and Sons Inc. 2022-03-17 2022-08 /pmc/articles/PMC9544884/ /pubmed/35175666 http://dx.doi.org/10.1111/febs.16401 Text en © 2022 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Rabbers, Iraes
Bruggeman, Frank J.
Escherichia coli robustly expresses ATP synthase at growth rate‐maximizing concentrations
title Escherichia coli robustly expresses ATP synthase at growth rate‐maximizing concentrations
title_full Escherichia coli robustly expresses ATP synthase at growth rate‐maximizing concentrations
title_fullStr Escherichia coli robustly expresses ATP synthase at growth rate‐maximizing concentrations
title_full_unstemmed Escherichia coli robustly expresses ATP synthase at growth rate‐maximizing concentrations
title_short Escherichia coli robustly expresses ATP synthase at growth rate‐maximizing concentrations
title_sort escherichia coli robustly expresses atp synthase at growth rate‐maximizing concentrations
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9544884/
https://www.ncbi.nlm.nih.gov/pubmed/35175666
http://dx.doi.org/10.1111/febs.16401
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