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Liquid chromatography‐dielectric barrier discharge ionization mass spectrometry for the analysis of neutral lipids of archaeological interest
Dielectric barrier discharge ionization has gained attention in the last few years due to its versatility and the vast array of molecules that can be ionized. In this study, we report on the assessment of liquid chromatography coupled to dielectric barrier discharge ionization with mass spectrometry...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9544991/ https://www.ncbi.nlm.nih.gov/pubmed/35801641 http://dx.doi.org/10.1002/jssc.202200402 |
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author | Bouza, Marcos García‐Martínez, Julio Gilbert‐López, Bienvenida Moreno‐González, David Rocío‐Bautista, Priscilla Parras‐Guijarro, David Sánchez‐Vizcaino, Alberto Brandt, Sebastian García‐Reyes, Juan F. Molina‐Díaz, Antonio Franzke, Joachim |
author_facet | Bouza, Marcos García‐Martínez, Julio Gilbert‐López, Bienvenida Moreno‐González, David Rocío‐Bautista, Priscilla Parras‐Guijarro, David Sánchez‐Vizcaino, Alberto Brandt, Sebastian García‐Reyes, Juan F. Molina‐Díaz, Antonio Franzke, Joachim |
author_sort | Bouza, Marcos |
collection | PubMed |
description | Dielectric barrier discharge ionization has gained attention in the last few years due to its versatility and the vast array of molecules that can be ionized. In this study, we report on the assessment of liquid chromatography coupled to dielectric barrier discharge ionization with mass spectrometry for neutral lipid analysis. A set of different neutral lipid subclasses (triacylglycerides, diacylglycerides, and sterols) were selected for the study. The main species detected from our ionization source were [M‐H(2)O+H](+), [M+H](+) or [M‐R‐H(2)O+H](+), attributed to sterol dehydration, protonation or the fragmentation of an acyl chain accompanied by a water loss of the glycerolipids, respectively. In terms of sensitivity, the dielectric barrier discharge displayed overall improved abundances and comparable or better limits of quantitation than atmospheric pressure chemical ionization for both acylglycerols and sterols. As a case study, different archaeological samples with variable content in neutral lipids, particularly triacylglycerides, were studied. The identification was carried out by combining accurate mass and the tentative formula associated with the exact mass, retention time matching with standards, and additional structural information from in‐source fragmentation. The high degree of unsaturation and the presence of sterols revealed the potential vegetal origin of the material stored in the analyzed samples. |
format | Online Article Text |
id | pubmed-9544991 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95449912022-10-14 Liquid chromatography‐dielectric barrier discharge ionization mass spectrometry for the analysis of neutral lipids of archaeological interest Bouza, Marcos García‐Martínez, Julio Gilbert‐López, Bienvenida Moreno‐González, David Rocío‐Bautista, Priscilla Parras‐Guijarro, David Sánchez‐Vizcaino, Alberto Brandt, Sebastian García‐Reyes, Juan F. Molina‐Díaz, Antonio Franzke, Joachim J Sep Sci Liquid Chromatography Dielectric barrier discharge ionization has gained attention in the last few years due to its versatility and the vast array of molecules that can be ionized. In this study, we report on the assessment of liquid chromatography coupled to dielectric barrier discharge ionization with mass spectrometry for neutral lipid analysis. A set of different neutral lipid subclasses (triacylglycerides, diacylglycerides, and sterols) were selected for the study. The main species detected from our ionization source were [M‐H(2)O+H](+), [M+H](+) or [M‐R‐H(2)O+H](+), attributed to sterol dehydration, protonation or the fragmentation of an acyl chain accompanied by a water loss of the glycerolipids, respectively. In terms of sensitivity, the dielectric barrier discharge displayed overall improved abundances and comparable or better limits of quantitation than atmospheric pressure chemical ionization for both acylglycerols and sterols. As a case study, different archaeological samples with variable content in neutral lipids, particularly triacylglycerides, were studied. The identification was carried out by combining accurate mass and the tentative formula associated with the exact mass, retention time matching with standards, and additional structural information from in‐source fragmentation. The high degree of unsaturation and the presence of sterols revealed the potential vegetal origin of the material stored in the analyzed samples. John Wiley and Sons Inc. 2022-07-14 2022-08 /pmc/articles/PMC9544991/ /pubmed/35801641 http://dx.doi.org/10.1002/jssc.202200402 Text en © 2022 The Authors. Journal of Separation Science published by Wiley‐VCH GmbH. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Liquid Chromatography Bouza, Marcos García‐Martínez, Julio Gilbert‐López, Bienvenida Moreno‐González, David Rocío‐Bautista, Priscilla Parras‐Guijarro, David Sánchez‐Vizcaino, Alberto Brandt, Sebastian García‐Reyes, Juan F. Molina‐Díaz, Antonio Franzke, Joachim Liquid chromatography‐dielectric barrier discharge ionization mass spectrometry for the analysis of neutral lipids of archaeological interest |
title | Liquid chromatography‐dielectric barrier discharge ionization mass spectrometry for the analysis of neutral lipids of archaeological interest |
title_full | Liquid chromatography‐dielectric barrier discharge ionization mass spectrometry for the analysis of neutral lipids of archaeological interest |
title_fullStr | Liquid chromatography‐dielectric barrier discharge ionization mass spectrometry for the analysis of neutral lipids of archaeological interest |
title_full_unstemmed | Liquid chromatography‐dielectric barrier discharge ionization mass spectrometry for the analysis of neutral lipids of archaeological interest |
title_short | Liquid chromatography‐dielectric barrier discharge ionization mass spectrometry for the analysis of neutral lipids of archaeological interest |
title_sort | liquid chromatography‐dielectric barrier discharge ionization mass spectrometry for the analysis of neutral lipids of archaeological interest |
topic | Liquid Chromatography |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9544991/ https://www.ncbi.nlm.nih.gov/pubmed/35801641 http://dx.doi.org/10.1002/jssc.202200402 |
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