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Listeria monocytogenes two component system PieRS regulates secretion chaperones PrsA1 and PrsA2 and enhances bacterial translocation across the intestine

Listeria monocytogenes (Lm) is a widespread environmental Gram‐positive bacterium that can transition into a pathogen following ingestion by a susceptible host. To cross host barriers and establish infection, Lm is dependent upon the regulated secretion and activity of many proteins including PrsA2,...

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Autores principales: Cahoon, Laty A., Alejandro‐Navarreto, Xiomarie, Gururaja, Avinash N., Light, Sam H., Alonzo, Francis, Anderson, Wayne F., Freitag, Nancy E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9545042/
https://www.ncbi.nlm.nih.gov/pubmed/35943959
http://dx.doi.org/10.1111/mmi.14967
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author Cahoon, Laty A.
Alejandro‐Navarreto, Xiomarie
Gururaja, Avinash N.
Light, Sam H.
Alonzo, Francis
Anderson, Wayne F.
Freitag, Nancy E.
author_facet Cahoon, Laty A.
Alejandro‐Navarreto, Xiomarie
Gururaja, Avinash N.
Light, Sam H.
Alonzo, Francis
Anderson, Wayne F.
Freitag, Nancy E.
author_sort Cahoon, Laty A.
collection PubMed
description Listeria monocytogenes (Lm) is a widespread environmental Gram‐positive bacterium that can transition into a pathogen following ingestion by a susceptible host. To cross host barriers and establish infection, Lm is dependent upon the regulated secretion and activity of many proteins including PrsA2, a peptidyl‐prolyl cis‐trans isomerase with foldase activity. PrsA2 contributes to the stability and activity of a number of secreted virulence factors that are required for Lm invasion, replication, and cell‐to‐cell spread within the infected host. In contrast, a second related secretion chaperone, PrsA1, has thus far no identified contributions to Lm pathogenesis. Here we describe the characterization of a two‐component signal transduction system PieRS that regulates the expression of a regulon that includes the secretion chaperones PrsA1 and PrsA2. PieRS regulated gene products are required for bacterial resistance to ethanol exposure and are important for bacterial survival during transit through the gastrointestinal tract. PrsA1 was also found to make a unique contribution to Lm survival in the GI tract, revealing for the first time a non‐overlapping requirement for both secretion chaperones PrsA1 and PrsA2 during the process of intra‐gastric infection.
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spelling pubmed-95450422022-10-14 Listeria monocytogenes two component system PieRS regulates secretion chaperones PrsA1 and PrsA2 and enhances bacterial translocation across the intestine Cahoon, Laty A. Alejandro‐Navarreto, Xiomarie Gururaja, Avinash N. Light, Sam H. Alonzo, Francis Anderson, Wayne F. Freitag, Nancy E. Mol Microbiol Research Articles Listeria monocytogenes (Lm) is a widespread environmental Gram‐positive bacterium that can transition into a pathogen following ingestion by a susceptible host. To cross host barriers and establish infection, Lm is dependent upon the regulated secretion and activity of many proteins including PrsA2, a peptidyl‐prolyl cis‐trans isomerase with foldase activity. PrsA2 contributes to the stability and activity of a number of secreted virulence factors that are required for Lm invasion, replication, and cell‐to‐cell spread within the infected host. In contrast, a second related secretion chaperone, PrsA1, has thus far no identified contributions to Lm pathogenesis. Here we describe the characterization of a two‐component signal transduction system PieRS that regulates the expression of a regulon that includes the secretion chaperones PrsA1 and PrsA2. PieRS regulated gene products are required for bacterial resistance to ethanol exposure and are important for bacterial survival during transit through the gastrointestinal tract. PrsA1 was also found to make a unique contribution to Lm survival in the GI tract, revealing for the first time a non‐overlapping requirement for both secretion chaperones PrsA1 and PrsA2 during the process of intra‐gastric infection. John Wiley and Sons Inc. 2022-08-09 2022-09 /pmc/articles/PMC9545042/ /pubmed/35943959 http://dx.doi.org/10.1111/mmi.14967 Text en © 2022 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Cahoon, Laty A.
Alejandro‐Navarreto, Xiomarie
Gururaja, Avinash N.
Light, Sam H.
Alonzo, Francis
Anderson, Wayne F.
Freitag, Nancy E.
Listeria monocytogenes two component system PieRS regulates secretion chaperones PrsA1 and PrsA2 and enhances bacterial translocation across the intestine
title Listeria monocytogenes two component system PieRS regulates secretion chaperones PrsA1 and PrsA2 and enhances bacterial translocation across the intestine
title_full Listeria monocytogenes two component system PieRS regulates secretion chaperones PrsA1 and PrsA2 and enhances bacterial translocation across the intestine
title_fullStr Listeria monocytogenes two component system PieRS regulates secretion chaperones PrsA1 and PrsA2 and enhances bacterial translocation across the intestine
title_full_unstemmed Listeria monocytogenes two component system PieRS regulates secretion chaperones PrsA1 and PrsA2 and enhances bacterial translocation across the intestine
title_short Listeria monocytogenes two component system PieRS regulates secretion chaperones PrsA1 and PrsA2 and enhances bacterial translocation across the intestine
title_sort listeria monocytogenes two component system piers regulates secretion chaperones prsa1 and prsa2 and enhances bacterial translocation across the intestine
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9545042/
https://www.ncbi.nlm.nih.gov/pubmed/35943959
http://dx.doi.org/10.1111/mmi.14967
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