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Effect of the most common wound antiseptics on human skin fibroblasts

BACKGROUND: Antiseptics are used for the cleansing of acute or chronic wounds to eliminate micro‐organisms from the wound bed. However, they have effects on the skin cells. AIM: To determine the effects of hexetidine, povidone–iodine (PI), undecylenamidopropyl‐betaine/polyhexanide (UBP), chlorhexidi...

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Autores principales: Rueda‐Fernández, Manuel, Melguizo‐Rodríguez, Lucía, Costela‐Ruiz, Víctor J., de Luna‐Bertos, Elvira, Ruiz, Concepción, Ramos‐Torrecillas, Javier, Illescas‐Montes, Rebeca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9545306/
https://www.ncbi.nlm.nih.gov/pubmed/35466431
http://dx.doi.org/10.1111/ced.15235
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author Rueda‐Fernández, Manuel
Melguizo‐Rodríguez, Lucía
Costela‐Ruiz, Víctor J.
de Luna‐Bertos, Elvira
Ruiz, Concepción
Ramos‐Torrecillas, Javier
Illescas‐Montes, Rebeca
author_facet Rueda‐Fernández, Manuel
Melguizo‐Rodríguez, Lucía
Costela‐Ruiz, Víctor J.
de Luna‐Bertos, Elvira
Ruiz, Concepción
Ramos‐Torrecillas, Javier
Illescas‐Montes, Rebeca
author_sort Rueda‐Fernández, Manuel
collection PubMed
description BACKGROUND: Antiseptics are used for the cleansing of acute or chronic wounds to eliminate micro‐organisms from the wound bed. However, they have effects on the skin cells. AIM: To determine the effects of hexetidine, povidone–iodine (PI), undecylenamidopropyl‐betaine/polyhexanide (UBP), chlorhexidine, disodium eosin and hydrogen peroxide on human skin fibroblasts. METHODS: CCD‐1064Sk cells were treated with hexetidine, PI, UBP, chlorhexidine, disodium eosin or hydrogen peroxide. Spectrophotometry was used to measure cell viability and flow cytometry was used to study apoptosis and necrosis after the treatment. In vitro wound scratch assays were performed to determine the gap closure. RESULTS: All antiseptics significantly reduced the viability of human skin fibroblasts compared with controls. The percentage wound closure was lower with hexetidine, PI and UBP. The scratch assay could not be measured after treatments with chlorhexidine, disodium eosin or hydrogen peroxide, owing to their cytotoxicity. The apoptosis/necrosis experiments evidenced a significant reduction in viable cells compared with controls. An increased percentage of apoptotic cells was observed after treatment with all antiseptics. Compared with controls, the percentage of necrotic cells was significantly increased with all antiseptics except for hexetidine. CONCLUSION: The proliferation, migration and viability of human skin fibroblasts are reduced by treatment with hexetidine, PI, UBP, chlorhexidine, disodium eosin and hydrogen peroxide.
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spelling pubmed-95453062022-10-14 Effect of the most common wound antiseptics on human skin fibroblasts Rueda‐Fernández, Manuel Melguizo‐Rodríguez, Lucía Costela‐Ruiz, Víctor J. de Luna‐Bertos, Elvira Ruiz, Concepción Ramos‐Torrecillas, Javier Illescas‐Montes, Rebeca Clin Exp Dermatol Original Articles BACKGROUND: Antiseptics are used for the cleansing of acute or chronic wounds to eliminate micro‐organisms from the wound bed. However, they have effects on the skin cells. AIM: To determine the effects of hexetidine, povidone–iodine (PI), undecylenamidopropyl‐betaine/polyhexanide (UBP), chlorhexidine, disodium eosin and hydrogen peroxide on human skin fibroblasts. METHODS: CCD‐1064Sk cells were treated with hexetidine, PI, UBP, chlorhexidine, disodium eosin or hydrogen peroxide. Spectrophotometry was used to measure cell viability and flow cytometry was used to study apoptosis and necrosis after the treatment. In vitro wound scratch assays were performed to determine the gap closure. RESULTS: All antiseptics significantly reduced the viability of human skin fibroblasts compared with controls. The percentage wound closure was lower with hexetidine, PI and UBP. The scratch assay could not be measured after treatments with chlorhexidine, disodium eosin or hydrogen peroxide, owing to their cytotoxicity. The apoptosis/necrosis experiments evidenced a significant reduction in viable cells compared with controls. An increased percentage of apoptotic cells was observed after treatment with all antiseptics. Compared with controls, the percentage of necrotic cells was significantly increased with all antiseptics except for hexetidine. CONCLUSION: The proliferation, migration and viability of human skin fibroblasts are reduced by treatment with hexetidine, PI, UBP, chlorhexidine, disodium eosin and hydrogen peroxide. John Wiley and Sons Inc. 2022-06-16 2022-08 /pmc/articles/PMC9545306/ /pubmed/35466431 http://dx.doi.org/10.1111/ced.15235 Text en © 2022 The Authors. Clinical and Experimental Dermatology published by John Wiley & Sons Ltd on behalf of British Association of Dermatologists. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Rueda‐Fernández, Manuel
Melguizo‐Rodríguez, Lucía
Costela‐Ruiz, Víctor J.
de Luna‐Bertos, Elvira
Ruiz, Concepción
Ramos‐Torrecillas, Javier
Illescas‐Montes, Rebeca
Effect of the most common wound antiseptics on human skin fibroblasts
title Effect of the most common wound antiseptics on human skin fibroblasts
title_full Effect of the most common wound antiseptics on human skin fibroblasts
title_fullStr Effect of the most common wound antiseptics on human skin fibroblasts
title_full_unstemmed Effect of the most common wound antiseptics on human skin fibroblasts
title_short Effect of the most common wound antiseptics on human skin fibroblasts
title_sort effect of the most common wound antiseptics on human skin fibroblasts
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9545306/
https://www.ncbi.nlm.nih.gov/pubmed/35466431
http://dx.doi.org/10.1111/ced.15235
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