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Bridging the gap: Short structural variants in the genetics of anorexia nervosa

Anorexia nervosa (AN) is a devastating disorder with evidence of underexplored heritability. Twin and family studies estimate heritability (h (2)) to be 57%–64%, and genome‐wide association studies (GWAS) reveal significant genetic correlations with psychiatric and anthropometric traits and a total...

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Autores principales: Berthold, Natasha, Pytte, Julia, Bulik, Cynthia M., Tschochner, Monika, Medland, Sarah E., Akkari, Patrick Anthony
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9545787/
https://www.ncbi.nlm.nih.gov/pubmed/35470453
http://dx.doi.org/10.1002/eat.23716
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author Berthold, Natasha
Pytte, Julia
Bulik, Cynthia M.
Tschochner, Monika
Medland, Sarah E.
Akkari, Patrick Anthony
author_facet Berthold, Natasha
Pytte, Julia
Bulik, Cynthia M.
Tschochner, Monika
Medland, Sarah E.
Akkari, Patrick Anthony
author_sort Berthold, Natasha
collection PubMed
description Anorexia nervosa (AN) is a devastating disorder with evidence of underexplored heritability. Twin and family studies estimate heritability (h (2)) to be 57%–64%, and genome‐wide association studies (GWAS) reveal significant genetic correlations with psychiatric and anthropometric traits and a total of nine genome‐wide significant loci. Whether significantly associated single nucleotide polymorphisms identified by GWAS are causal or tag true causal variants, remains to be elucidated. We propose a novel method for bridging this knowledge gap by fine‐mapping short structural variants (SSVs) in and around GWAS‐identified loci. SSV fine‐mapping of loci associated with complex disorders such as schizophrenia, amyotrophic lateral sclerosis, and Alzheimer's disease has uncovered genetic risk markers, phenotypic variability between patients, new pathological mechanisms, and potential therapeutic targets. We analyze previous investigations' methods and propose utilizing an evaluation algorithm to prioritize 10 SSVs for each of the top two AN GWAS‐identified loci followed by Sanger sequencing and fragment analysis via capillary electrophoresis to characterize these SSVs for case/control association studies. Success of previous SSV analyses in complex disorders and effective utilization of similar methodologies supports our proposed method. Furthermore, the structural and spatial properties of the 10 SSVs identified for each of the top two AN GWAS‐associated loci, cell adhesion molecule 1 (CADM1) and NCK interacting protein with SH3 domain (NCKIPSD), are similar to previous studies. We propose SSV fine‐mapping of AN‐associated loci will identify causal genetic architecture. Deepening understandings of AN may lead to novel therapeutic targets and subsequently increase quality‐of‐life for individuals living with the illness. PUBLIC SIGNIFICANCE STATEMENT: Anorexia nervosa is a severe and complex illness, arising from a combination of environmental and genetic factors. Recent studies estimate the contribution of genetic variability; however, the specific DNA sequences and how they contribute remain unknown. We present a novel approach, arguing that the genetic variant class, short structural variants, could answer this knowledge gap and allow development of biologically targeted therapeutics, improving quality‐of‐life and patient outcomes for affected individuals.
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spelling pubmed-95457872022-10-14 Bridging the gap: Short structural variants in the genetics of anorexia nervosa Berthold, Natasha Pytte, Julia Bulik, Cynthia M. Tschochner, Monika Medland, Sarah E. Akkari, Patrick Anthony Int J Eat Disord Spotlight Anorexia nervosa (AN) is a devastating disorder with evidence of underexplored heritability. Twin and family studies estimate heritability (h (2)) to be 57%–64%, and genome‐wide association studies (GWAS) reveal significant genetic correlations with psychiatric and anthropometric traits and a total of nine genome‐wide significant loci. Whether significantly associated single nucleotide polymorphisms identified by GWAS are causal or tag true causal variants, remains to be elucidated. We propose a novel method for bridging this knowledge gap by fine‐mapping short structural variants (SSVs) in and around GWAS‐identified loci. SSV fine‐mapping of loci associated with complex disorders such as schizophrenia, amyotrophic lateral sclerosis, and Alzheimer's disease has uncovered genetic risk markers, phenotypic variability between patients, new pathological mechanisms, and potential therapeutic targets. We analyze previous investigations' methods and propose utilizing an evaluation algorithm to prioritize 10 SSVs for each of the top two AN GWAS‐identified loci followed by Sanger sequencing and fragment analysis via capillary electrophoresis to characterize these SSVs for case/control association studies. Success of previous SSV analyses in complex disorders and effective utilization of similar methodologies supports our proposed method. Furthermore, the structural and spatial properties of the 10 SSVs identified for each of the top two AN GWAS‐associated loci, cell adhesion molecule 1 (CADM1) and NCK interacting protein with SH3 domain (NCKIPSD), are similar to previous studies. We propose SSV fine‐mapping of AN‐associated loci will identify causal genetic architecture. Deepening understandings of AN may lead to novel therapeutic targets and subsequently increase quality‐of‐life for individuals living with the illness. PUBLIC SIGNIFICANCE STATEMENT: Anorexia nervosa is a severe and complex illness, arising from a combination of environmental and genetic factors. Recent studies estimate the contribution of genetic variability; however, the specific DNA sequences and how they contribute remain unknown. We present a novel approach, arguing that the genetic variant class, short structural variants, could answer this knowledge gap and allow development of biologically targeted therapeutics, improving quality‐of‐life and patient outcomes for affected individuals. John Wiley & Sons, Inc. 2022-04-26 2022-06 /pmc/articles/PMC9545787/ /pubmed/35470453 http://dx.doi.org/10.1002/eat.23716 Text en © 2022 The Authors. International Journal of Eating Disorders published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Spotlight
Berthold, Natasha
Pytte, Julia
Bulik, Cynthia M.
Tschochner, Monika
Medland, Sarah E.
Akkari, Patrick Anthony
Bridging the gap: Short structural variants in the genetics of anorexia nervosa
title Bridging the gap: Short structural variants in the genetics of anorexia nervosa
title_full Bridging the gap: Short structural variants in the genetics of anorexia nervosa
title_fullStr Bridging the gap: Short structural variants in the genetics of anorexia nervosa
title_full_unstemmed Bridging the gap: Short structural variants in the genetics of anorexia nervosa
title_short Bridging the gap: Short structural variants in the genetics of anorexia nervosa
title_sort bridging the gap: short structural variants in the genetics of anorexia nervosa
topic Spotlight
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9545787/
https://www.ncbi.nlm.nih.gov/pubmed/35470453
http://dx.doi.org/10.1002/eat.23716
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