Cargando…
Activation of TREK1 Channel in the Anterior Cingulate Cortex Improves Neuropathic Pain in a Rat Model
Objective. To explore the biological function and mechanism of TREK1 in neuropathic pain. Thirty-two healthy rats and rats with sciatic nerve chronic press-fitting model (chronic constriction injury of the sciatic nerve, CCI) were selected. Western blot, immunofluorescence staining, and patch clamp...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9546664/ https://www.ncbi.nlm.nih.gov/pubmed/36211011 http://dx.doi.org/10.1155/2022/1372823 |
_version_ | 1784805093013454848 |
---|---|
author | Peng, Yuanzhi Zhang, Qingqing Cheng, Hao Shen, Saie Weng, Xiaojian |
author_facet | Peng, Yuanzhi Zhang, Qingqing Cheng, Hao Shen, Saie Weng, Xiaojian |
author_sort | Peng, Yuanzhi |
collection | PubMed |
description | Objective. To explore the biological function and mechanism of TREK1 in neuropathic pain. Thirty-two healthy rats and rats with sciatic nerve chronic press-fitting model (chronic constriction injury of the sciatic nerve, CCI) were selected. Western blot, immunofluorescence staining, and patch clamp technique were performed to explore the biological functions of TREK1. The expression of TREK1 was decreased in the CCI model. The TREK1 channel current in the CCI model was decreased. After local administration of TREK1 channel activator in the anterior cingulate cortex area, the pain behavior of CCI rats and the expression of TREK1 protein were reversed. The expression of TREK1 was downregulated in the ACC area of CCI rats and the current of TREK1 was decreased, which played an important role in the regulation of neuropathic pain. |
format | Online Article Text |
id | pubmed-9546664 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-95466642022-10-08 Activation of TREK1 Channel in the Anterior Cingulate Cortex Improves Neuropathic Pain in a Rat Model Peng, Yuanzhi Zhang, Qingqing Cheng, Hao Shen, Saie Weng, Xiaojian Comput Intell Neurosci Research Article Objective. To explore the biological function and mechanism of TREK1 in neuropathic pain. Thirty-two healthy rats and rats with sciatic nerve chronic press-fitting model (chronic constriction injury of the sciatic nerve, CCI) were selected. Western blot, immunofluorescence staining, and patch clamp technique were performed to explore the biological functions of TREK1. The expression of TREK1 was decreased in the CCI model. The TREK1 channel current in the CCI model was decreased. After local administration of TREK1 channel activator in the anterior cingulate cortex area, the pain behavior of CCI rats and the expression of TREK1 protein were reversed. The expression of TREK1 was downregulated in the ACC area of CCI rats and the current of TREK1 was decreased, which played an important role in the regulation of neuropathic pain. Hindawi 2022-09-30 /pmc/articles/PMC9546664/ /pubmed/36211011 http://dx.doi.org/10.1155/2022/1372823 Text en Copyright © 2022 Yuanzhi Peng et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Peng, Yuanzhi Zhang, Qingqing Cheng, Hao Shen, Saie Weng, Xiaojian Activation of TREK1 Channel in the Anterior Cingulate Cortex Improves Neuropathic Pain in a Rat Model |
title | Activation of TREK1 Channel in the Anterior Cingulate Cortex Improves Neuropathic Pain in a Rat Model |
title_full | Activation of TREK1 Channel in the Anterior Cingulate Cortex Improves Neuropathic Pain in a Rat Model |
title_fullStr | Activation of TREK1 Channel in the Anterior Cingulate Cortex Improves Neuropathic Pain in a Rat Model |
title_full_unstemmed | Activation of TREK1 Channel in the Anterior Cingulate Cortex Improves Neuropathic Pain in a Rat Model |
title_short | Activation of TREK1 Channel in the Anterior Cingulate Cortex Improves Neuropathic Pain in a Rat Model |
title_sort | activation of trek1 channel in the anterior cingulate cortex improves neuropathic pain in a rat model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9546664/ https://www.ncbi.nlm.nih.gov/pubmed/36211011 http://dx.doi.org/10.1155/2022/1372823 |
work_keys_str_mv | AT pengyuanzhi activationoftrek1channelintheanteriorcingulatecorteximprovesneuropathicpaininaratmodel AT zhangqingqing activationoftrek1channelintheanteriorcingulatecorteximprovesneuropathicpaininaratmodel AT chenghao activationoftrek1channelintheanteriorcingulatecorteximprovesneuropathicpaininaratmodel AT shensaie activationoftrek1channelintheanteriorcingulatecorteximprovesneuropathicpaininaratmodel AT wengxiaojian activationoftrek1channelintheanteriorcingulatecorteximprovesneuropathicpaininaratmodel |