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MicroRNA-641 Inhibits Endometrial Cancer Progression via Targeting AP1G1

MicroRNA-641 (miR-641) was significantly decreased in various cancers, but its roles in endometrial cancer (EC) remain unclear. We explored the influences of miR-641 on the EC cells. In our study, the miR-641 expression was reduced in EC cells. Overexpression of miR-641 inhibited viability and proli...

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Detalles Bibliográficos
Autores principales: Dong, Yanfen, Yang, He, Hua, Handan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9546697/
https://www.ncbi.nlm.nih.gov/pubmed/36212964
http://dx.doi.org/10.1155/2022/7918596
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author Dong, Yanfen
Yang, He
Hua, Handan
author_facet Dong, Yanfen
Yang, He
Hua, Handan
author_sort Dong, Yanfen
collection PubMed
description MicroRNA-641 (miR-641) was significantly decreased in various cancers, but its roles in endometrial cancer (EC) remain unclear. We explored the influences of miR-641 on the EC cells. In our study, the miR-641 expression was reduced in EC cells. Overexpression of miR-641 inhibited viability and proliferation of HEC-1A and HECCL-1 cells by CCK-8 and colony formation assays. Additionally, flow cytometry revealed that overexpression of miR-641 could remarkably promote apoptosis and arrest the cell cycle at the G1 phase of HEC-1A and HECCL-1 cells. Besides, forced expression of miR-641 suppressed the migration and invasion of HEC-1A and HECCL-1 cells as evidenced by wound healing and transwell assay. Moreover, AP1G1 was confirmed as a target gene of miR-641 by StarBase prediction and DLR assay and their expressions were negatively correlated. Overexpression of AP1G1 neutralized the roles of miR-641 mimic on the viability, proliferation, apoptosis, and migration of HEC-1A and HECCL-1 cells. Our findings illustrated that miR-641 was reduced in the EC cells and AP1G1 antagonized the miR-641 mimic-induced inhibition of the EC progression in vitro. Therefore, miR-641 may emerge as an effective molecule for EC treatment.
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spelling pubmed-95466972022-10-08 MicroRNA-641 Inhibits Endometrial Cancer Progression via Targeting AP1G1 Dong, Yanfen Yang, He Hua, Handan Evid Based Complement Alternat Med Research Article MicroRNA-641 (miR-641) was significantly decreased in various cancers, but its roles in endometrial cancer (EC) remain unclear. We explored the influences of miR-641 on the EC cells. In our study, the miR-641 expression was reduced in EC cells. Overexpression of miR-641 inhibited viability and proliferation of HEC-1A and HECCL-1 cells by CCK-8 and colony formation assays. Additionally, flow cytometry revealed that overexpression of miR-641 could remarkably promote apoptosis and arrest the cell cycle at the G1 phase of HEC-1A and HECCL-1 cells. Besides, forced expression of miR-641 suppressed the migration and invasion of HEC-1A and HECCL-1 cells as evidenced by wound healing and transwell assay. Moreover, AP1G1 was confirmed as a target gene of miR-641 by StarBase prediction and DLR assay and their expressions were negatively correlated. Overexpression of AP1G1 neutralized the roles of miR-641 mimic on the viability, proliferation, apoptosis, and migration of HEC-1A and HECCL-1 cells. Our findings illustrated that miR-641 was reduced in the EC cells and AP1G1 antagonized the miR-641 mimic-induced inhibition of the EC progression in vitro. Therefore, miR-641 may emerge as an effective molecule for EC treatment. Hindawi 2022-09-30 /pmc/articles/PMC9546697/ /pubmed/36212964 http://dx.doi.org/10.1155/2022/7918596 Text en Copyright © 2022 Yanfen Dong et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dong, Yanfen
Yang, He
Hua, Handan
MicroRNA-641 Inhibits Endometrial Cancer Progression via Targeting AP1G1
title MicroRNA-641 Inhibits Endometrial Cancer Progression via Targeting AP1G1
title_full MicroRNA-641 Inhibits Endometrial Cancer Progression via Targeting AP1G1
title_fullStr MicroRNA-641 Inhibits Endometrial Cancer Progression via Targeting AP1G1
title_full_unstemmed MicroRNA-641 Inhibits Endometrial Cancer Progression via Targeting AP1G1
title_short MicroRNA-641 Inhibits Endometrial Cancer Progression via Targeting AP1G1
title_sort microrna-641 inhibits endometrial cancer progression via targeting ap1g1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9546697/
https://www.ncbi.nlm.nih.gov/pubmed/36212964
http://dx.doi.org/10.1155/2022/7918596
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