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MicroRNA-641 Inhibits Endometrial Cancer Progression via Targeting AP1G1
MicroRNA-641 (miR-641) was significantly decreased in various cancers, but its roles in endometrial cancer (EC) remain unclear. We explored the influences of miR-641 on the EC cells. In our study, the miR-641 expression was reduced in EC cells. Overexpression of miR-641 inhibited viability and proli...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9546697/ https://www.ncbi.nlm.nih.gov/pubmed/36212964 http://dx.doi.org/10.1155/2022/7918596 |
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author | Dong, Yanfen Yang, He Hua, Handan |
author_facet | Dong, Yanfen Yang, He Hua, Handan |
author_sort | Dong, Yanfen |
collection | PubMed |
description | MicroRNA-641 (miR-641) was significantly decreased in various cancers, but its roles in endometrial cancer (EC) remain unclear. We explored the influences of miR-641 on the EC cells. In our study, the miR-641 expression was reduced in EC cells. Overexpression of miR-641 inhibited viability and proliferation of HEC-1A and HECCL-1 cells by CCK-8 and colony formation assays. Additionally, flow cytometry revealed that overexpression of miR-641 could remarkably promote apoptosis and arrest the cell cycle at the G1 phase of HEC-1A and HECCL-1 cells. Besides, forced expression of miR-641 suppressed the migration and invasion of HEC-1A and HECCL-1 cells as evidenced by wound healing and transwell assay. Moreover, AP1G1 was confirmed as a target gene of miR-641 by StarBase prediction and DLR assay and their expressions were negatively correlated. Overexpression of AP1G1 neutralized the roles of miR-641 mimic on the viability, proliferation, apoptosis, and migration of HEC-1A and HECCL-1 cells. Our findings illustrated that miR-641 was reduced in the EC cells and AP1G1 antagonized the miR-641 mimic-induced inhibition of the EC progression in vitro. Therefore, miR-641 may emerge as an effective molecule for EC treatment. |
format | Online Article Text |
id | pubmed-9546697 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-95466972022-10-08 MicroRNA-641 Inhibits Endometrial Cancer Progression via Targeting AP1G1 Dong, Yanfen Yang, He Hua, Handan Evid Based Complement Alternat Med Research Article MicroRNA-641 (miR-641) was significantly decreased in various cancers, but its roles in endometrial cancer (EC) remain unclear. We explored the influences of miR-641 on the EC cells. In our study, the miR-641 expression was reduced in EC cells. Overexpression of miR-641 inhibited viability and proliferation of HEC-1A and HECCL-1 cells by CCK-8 and colony formation assays. Additionally, flow cytometry revealed that overexpression of miR-641 could remarkably promote apoptosis and arrest the cell cycle at the G1 phase of HEC-1A and HECCL-1 cells. Besides, forced expression of miR-641 suppressed the migration and invasion of HEC-1A and HECCL-1 cells as evidenced by wound healing and transwell assay. Moreover, AP1G1 was confirmed as a target gene of miR-641 by StarBase prediction and DLR assay and their expressions were negatively correlated. Overexpression of AP1G1 neutralized the roles of miR-641 mimic on the viability, proliferation, apoptosis, and migration of HEC-1A and HECCL-1 cells. Our findings illustrated that miR-641 was reduced in the EC cells and AP1G1 antagonized the miR-641 mimic-induced inhibition of the EC progression in vitro. Therefore, miR-641 may emerge as an effective molecule for EC treatment. Hindawi 2022-09-30 /pmc/articles/PMC9546697/ /pubmed/36212964 http://dx.doi.org/10.1155/2022/7918596 Text en Copyright © 2022 Yanfen Dong et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Dong, Yanfen Yang, He Hua, Handan MicroRNA-641 Inhibits Endometrial Cancer Progression via Targeting AP1G1 |
title | MicroRNA-641 Inhibits Endometrial Cancer Progression via Targeting AP1G1 |
title_full | MicroRNA-641 Inhibits Endometrial Cancer Progression via Targeting AP1G1 |
title_fullStr | MicroRNA-641 Inhibits Endometrial Cancer Progression via Targeting AP1G1 |
title_full_unstemmed | MicroRNA-641 Inhibits Endometrial Cancer Progression via Targeting AP1G1 |
title_short | MicroRNA-641 Inhibits Endometrial Cancer Progression via Targeting AP1G1 |
title_sort | microrna-641 inhibits endometrial cancer progression via targeting ap1g1 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9546697/ https://www.ncbi.nlm.nih.gov/pubmed/36212964 http://dx.doi.org/10.1155/2022/7918596 |
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