Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq

We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease resource consump...

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Detalles Bibliográficos
Autores principales: Hahaut, Vincent, Pavlinic, Dinko, Carbone, Walter, Schuierer, Sven, Balmer, Pierre, Quinodoz, Mathieu, Renner, Magdalena, Roma, Guglielmo, Cowan, Cameron S., Picelli, Simone
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2022
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9546769/
https://www.ncbi.nlm.nih.gov/pubmed/35637419
http://dx.doi.org/10.1038/s41587-022-01312-3
Descripción
Sumario:We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease resource consumption. The FS protocol can also use unique molecular identifiers (UMIs) for molecule counting while displaying reduced strand-invasion artifacts. FS will be especially useful for characterizing gene expression at high resolution across multiple samples.

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