Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq

We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease resource consump...

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Autores principales: Hahaut, Vincent, Pavlinic, Dinko, Carbone, Walter, Schuierer, Sven, Balmer, Pierre, Quinodoz, Mathieu, Renner, Magdalena, Roma, Guglielmo, Cowan, Cameron S., Picelli, Simone
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2022
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9546769/
https://www.ncbi.nlm.nih.gov/pubmed/35637419
http://dx.doi.org/10.1038/s41587-022-01312-3
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author Hahaut, Vincent
Pavlinic, Dinko
Carbone, Walter
Schuierer, Sven
Balmer, Pierre
Quinodoz, Mathieu
Renner, Magdalena
Roma, Guglielmo
Cowan, Cameron S.
Picelli, Simone
author_facet Hahaut, Vincent
Pavlinic, Dinko
Carbone, Walter
Schuierer, Sven
Balmer, Pierre
Quinodoz, Mathieu
Renner, Magdalena
Roma, Guglielmo
Cowan, Cameron S.
Picelli, Simone
author_sort Hahaut, Vincent
collection PubMed
description We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease resource consumption. The FS protocol can also use unique molecular identifiers (UMIs) for molecule counting while displaying reduced strand-invasion artifacts. FS will be especially useful for characterizing gene expression at high resolution across multiple samples.
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spelling pubmed-95467692022-10-09 Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq Hahaut, Vincent Pavlinic, Dinko Carbone, Walter Schuierer, Sven Balmer, Pierre Quinodoz, Mathieu Renner, Magdalena Roma, Guglielmo Cowan, Cameron S. Picelli, Simone Nat Biotechnol Brief Communication We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease resource consumption. The FS protocol can also use unique molecular identifiers (UMIs) for molecule counting while displaying reduced strand-invasion artifacts. FS will be especially useful for characterizing gene expression at high resolution across multiple samples. Nature Publishing Group US 2022-05-30 2022 /pmc/articles/PMC9546769/ /pubmed/35637419 http://dx.doi.org/10.1038/s41587-022-01312-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Brief Communication
Hahaut, Vincent
Pavlinic, Dinko
Carbone, Walter
Schuierer, Sven
Balmer, Pierre
Quinodoz, Mathieu
Renner, Magdalena
Roma, Guglielmo
Cowan, Cameron S.
Picelli, Simone
Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq
title Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq
title_full Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq
title_fullStr Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq
title_full_unstemmed Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq
title_short Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq
title_sort fast and highly sensitive full-length single-cell rna sequencing using flash-seq
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9546769/
https://www.ncbi.nlm.nih.gov/pubmed/35637419
http://dx.doi.org/10.1038/s41587-022-01312-3
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