DCAF-13 is required for C. elegans growth, development, and fertility

DCAF13 (DDB1 and CUL4 associated factor 13) is a potential oncogene but little is understood about the developmental roles of this highly conserved gene. We characterized the RNAi phenotypes of dcaf-13 , the C. elegans homolog of DCAF13, and show that compared to age-matched control worms, body leng...

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Autores principales: Durkan, Aidan, Byrnes, Catherine, Cooper, Emily, Hally, Alyson, Sullivan-Brown, Jessica, Sowa, Jessica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9547275/
https://www.ncbi.nlm.nih.gov/pubmed/36217444
http://dx.doi.org/10.17912/micropub.biology.000641
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author Durkan, Aidan
Byrnes, Catherine
Cooper, Emily
Hally, Alyson
Sullivan-Brown, Jessica
Sowa, Jessica
author_facet Durkan, Aidan
Byrnes, Catherine
Cooper, Emily
Hally, Alyson
Sullivan-Brown, Jessica
Sowa, Jessica
author_sort Durkan, Aidan
collection PubMed
description DCAF13 (DDB1 and CUL4 associated factor 13) is a potential oncogene but little is understood about the developmental roles of this highly conserved gene. We characterized the RNAi phenotypes of dcaf-13 , the C. elegans homolog of DCAF13, and show that compared to age-matched control worms, body length is decreased in dcaf-13 (RNAi) C. elegans larvae, suggesting a role of dcaf-13 in larval development. In addition, dcaf-13 (RNAi) worms display either a failure or delay in reaching the L4 and adult stages. Our data also indicates that dcaf-13 (RNAi) treatment beginning at L4 stage does not increase embryonic lethality in progeny; however, progeny production was significantly decreased in dcaf-13 (RNAi) worms, suggesting a general role in fertility and perhaps oocyte development.
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spelling pubmed-95472752022-10-09 DCAF-13 is required for C. elegans growth, development, and fertility Durkan, Aidan Byrnes, Catherine Cooper, Emily Hally, Alyson Sullivan-Brown, Jessica Sowa, Jessica MicroPubl Biol New Finding DCAF13 (DDB1 and CUL4 associated factor 13) is a potential oncogene but little is understood about the developmental roles of this highly conserved gene. We characterized the RNAi phenotypes of dcaf-13 , the C. elegans homolog of DCAF13, and show that compared to age-matched control worms, body length is decreased in dcaf-13 (RNAi) C. elegans larvae, suggesting a role of dcaf-13 in larval development. In addition, dcaf-13 (RNAi) worms display either a failure or delay in reaching the L4 and adult stages. Our data also indicates that dcaf-13 (RNAi) treatment beginning at L4 stage does not increase embryonic lethality in progeny; however, progeny production was significantly decreased in dcaf-13 (RNAi) worms, suggesting a general role in fertility and perhaps oocyte development. Caltech Library 2022-09-23 /pmc/articles/PMC9547275/ /pubmed/36217444 http://dx.doi.org/10.17912/micropub.biology.000641 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Durkan, Aidan
Byrnes, Catherine
Cooper, Emily
Hally, Alyson
Sullivan-Brown, Jessica
Sowa, Jessica
DCAF-13 is required for C. elegans growth, development, and fertility
title DCAF-13 is required for C. elegans growth, development, and fertility
title_full DCAF-13 is required for C. elegans growth, development, and fertility
title_fullStr DCAF-13 is required for C. elegans growth, development, and fertility
title_full_unstemmed DCAF-13 is required for C. elegans growth, development, and fertility
title_short DCAF-13 is required for C. elegans growth, development, and fertility
title_sort dcaf-13 is required for c. elegans growth, development, and fertility
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9547275/
https://www.ncbi.nlm.nih.gov/pubmed/36217444
http://dx.doi.org/10.17912/micropub.biology.000641
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