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Molecular epidemiology of human adenoviruses in children with and without respiratory symptoms: Preliminary findings from a case-control study
BACKGROUND: Human adenovirus (HAdV) is an important viral agent in children which can lead to severe acute respiratory infection (SARI). Reports on molecular epidemiology of HAdVs in Iran are limited. This case-control study is conducted to compare the HAdV infection rate and molecular epidemiology...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9547415/ https://www.ncbi.nlm.nih.gov/pubmed/36207696 http://dx.doi.org/10.1186/s12887-022-03625-3 |
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author | Zadheidar, Sevrin Yavarian, Jila Heydarifard, Zahra Nejati, Ahmad Sadeghi, Kaveh Ghavami, Nastaran Abbasi, Simin Shatizadeh Malekshahi, Somayeh Mokhtari-Azad, Talat Shafiei-Jandaghi, Nazanin-Zahra |
author_facet | Zadheidar, Sevrin Yavarian, Jila Heydarifard, Zahra Nejati, Ahmad Sadeghi, Kaveh Ghavami, Nastaran Abbasi, Simin Shatizadeh Malekshahi, Somayeh Mokhtari-Azad, Talat Shafiei-Jandaghi, Nazanin-Zahra |
author_sort | Zadheidar, Sevrin |
collection | PubMed |
description | BACKGROUND: Human adenovirus (HAdV) is an important viral agent in children which can lead to severe acute respiratory infection (SARI). Reports on molecular epidemiology of HAdVs in Iran are limited. This case-control study is conducted to compare the HAdV infection rate and molecular epidemiology among two groups of children with and without respiratory symptoms in Tehran, Iran during 2018–2019. METHODS: Nested PCR was performed on 120 oropharyngeal swabs taken from children aged five and younger with SARI who were hospitalized as the case group, and 120 oropharyngeal swabs were collected from children of the same age without respiratory symptoms as the control group. For positive samples Sanger sequencing was done and a phylogenetic tree was drawn afterward. RESULTS: Out of 120 cases, 8 (6.6%) tested positive for eachHAdV types including 6 (75%) HAdV-B7, 1 (12.5%) HAdV-C2, and 1 (12.5%) HAdV-C6. Among the control group, out of 120 samples, 8 (6.6%) were positive comprising 5 (62.5%) HAdV-C5, 2 (25%) HAdV-F41, and 1 (12.5%) HAdV-C6. CONCLUSION: The present study indicated a different viewpoint of HAdV molecular epidemiology in which the genotypes were compared in children with and without respiratory symptoms. HAdV prevalence was equally common in cases and controls but different genotypes were detected in these two groups. HAdV-B7 was the main type among children with SARI, dissimilar to children with no respiratory symptoms where HAdV-C5 was the predominant type. Detecting HAdV-F in oropharyngeal swabs was a rare finding, which requires further investigation. |
format | Online Article Text |
id | pubmed-9547415 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-95474152022-10-09 Molecular epidemiology of human adenoviruses in children with and without respiratory symptoms: Preliminary findings from a case-control study Zadheidar, Sevrin Yavarian, Jila Heydarifard, Zahra Nejati, Ahmad Sadeghi, Kaveh Ghavami, Nastaran Abbasi, Simin Shatizadeh Malekshahi, Somayeh Mokhtari-Azad, Talat Shafiei-Jandaghi, Nazanin-Zahra BMC Pediatr Research BACKGROUND: Human adenovirus (HAdV) is an important viral agent in children which can lead to severe acute respiratory infection (SARI). Reports on molecular epidemiology of HAdVs in Iran are limited. This case-control study is conducted to compare the HAdV infection rate and molecular epidemiology among two groups of children with and without respiratory symptoms in Tehran, Iran during 2018–2019. METHODS: Nested PCR was performed on 120 oropharyngeal swabs taken from children aged five and younger with SARI who were hospitalized as the case group, and 120 oropharyngeal swabs were collected from children of the same age without respiratory symptoms as the control group. For positive samples Sanger sequencing was done and a phylogenetic tree was drawn afterward. RESULTS: Out of 120 cases, 8 (6.6%) tested positive for eachHAdV types including 6 (75%) HAdV-B7, 1 (12.5%) HAdV-C2, and 1 (12.5%) HAdV-C6. Among the control group, out of 120 samples, 8 (6.6%) were positive comprising 5 (62.5%) HAdV-C5, 2 (25%) HAdV-F41, and 1 (12.5%) HAdV-C6. CONCLUSION: The present study indicated a different viewpoint of HAdV molecular epidemiology in which the genotypes were compared in children with and without respiratory symptoms. HAdV prevalence was equally common in cases and controls but different genotypes were detected in these two groups. HAdV-B7 was the main type among children with SARI, dissimilar to children with no respiratory symptoms where HAdV-C5 was the predominant type. Detecting HAdV-F in oropharyngeal swabs was a rare finding, which requires further investigation. BioMed Central 2022-10-08 /pmc/articles/PMC9547415/ /pubmed/36207696 http://dx.doi.org/10.1186/s12887-022-03625-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Zadheidar, Sevrin Yavarian, Jila Heydarifard, Zahra Nejati, Ahmad Sadeghi, Kaveh Ghavami, Nastaran Abbasi, Simin Shatizadeh Malekshahi, Somayeh Mokhtari-Azad, Talat Shafiei-Jandaghi, Nazanin-Zahra Molecular epidemiology of human adenoviruses in children with and without respiratory symptoms: Preliminary findings from a case-control study |
title | Molecular epidemiology of human adenoviruses in children with and without respiratory symptoms: Preliminary findings from a case-control study |
title_full | Molecular epidemiology of human adenoviruses in children with and without respiratory symptoms: Preliminary findings from a case-control study |
title_fullStr | Molecular epidemiology of human adenoviruses in children with and without respiratory symptoms: Preliminary findings from a case-control study |
title_full_unstemmed | Molecular epidemiology of human adenoviruses in children with and without respiratory symptoms: Preliminary findings from a case-control study |
title_short | Molecular epidemiology of human adenoviruses in children with and without respiratory symptoms: Preliminary findings from a case-control study |
title_sort | molecular epidemiology of human adenoviruses in children with and without respiratory symptoms: preliminary findings from a case-control study |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9547415/ https://www.ncbi.nlm.nih.gov/pubmed/36207696 http://dx.doi.org/10.1186/s12887-022-03625-3 |
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