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Multimodular vascularized bone construct comprised of vasculogenic and osteogenic microtissues

Bioengineered bone designed to heal large defects requires concomitant development of osseous and vascular tissue to ensure engraftment and survival. Adult human mesenchymal stromal cells (MSC) are promising in this application because they have demonstrated both osteogenic and vasculogenic potentia...

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Autores principales: Schott, Nicholas G., Vu, Huy, Stegemann, Jan P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9547967/
https://www.ncbi.nlm.nih.gov/pubmed/35922969
http://dx.doi.org/10.1002/bit.28201
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author Schott, Nicholas G.
Vu, Huy
Stegemann, Jan P.
author_facet Schott, Nicholas G.
Vu, Huy
Stegemann, Jan P.
author_sort Schott, Nicholas G.
collection PubMed
description Bioengineered bone designed to heal large defects requires concomitant development of osseous and vascular tissue to ensure engraftment and survival. Adult human mesenchymal stromal cells (MSC) are promising in this application because they have demonstrated both osteogenic and vasculogenic potential. This study employed a modular approach in which cells were encapsulated in biomaterial carriers (microtissues) designed to support tissue‐specific function. Osteogenic microtissues consisting of MSC embedded in a collagen‐chitosan matrix; vasculogenic (VAS) microtissues consisted of endothelial cells and MSC in a fibrin matrix. Microtissues were precultured under differentiation conditions to induce appropriate MSC lineage commitment, and were then combined in a surrounding fibrin hydrogel to create a multimodular construct. Results demonstrated the ability of microtissues to support lineage commitment, and that preculture primes the microtissues for the desired function. Combination of osteogenic and vasculogenic microtissues into multimodular constructs demonstrated that osteogenic priming resulted in sustained osteogenic activity even when cultured in vasculogenic medium, and that vasculogenic priming induced a pericyte‐like phenotype that resulted in development of a primitive vessel network in the constructs. The modular approach allows microtissues to be separately precultured to harness the dual differentiation potential of MSC to support both bone and blood vessel formation in a unified construct.
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spelling pubmed-95479672023-01-03 Multimodular vascularized bone construct comprised of vasculogenic and osteogenic microtissues Schott, Nicholas G. Vu, Huy Stegemann, Jan P. Biotechnol Bioeng ARTICLES Bioengineered bone designed to heal large defects requires concomitant development of osseous and vascular tissue to ensure engraftment and survival. Adult human mesenchymal stromal cells (MSC) are promising in this application because they have demonstrated both osteogenic and vasculogenic potential. This study employed a modular approach in which cells were encapsulated in biomaterial carriers (microtissues) designed to support tissue‐specific function. Osteogenic microtissues consisting of MSC embedded in a collagen‐chitosan matrix; vasculogenic (VAS) microtissues consisted of endothelial cells and MSC in a fibrin matrix. Microtissues were precultured under differentiation conditions to induce appropriate MSC lineage commitment, and were then combined in a surrounding fibrin hydrogel to create a multimodular construct. Results demonstrated the ability of microtissues to support lineage commitment, and that preculture primes the microtissues for the desired function. Combination of osteogenic and vasculogenic microtissues into multimodular constructs demonstrated that osteogenic priming resulted in sustained osteogenic activity even when cultured in vasculogenic medium, and that vasculogenic priming induced a pericyte‐like phenotype that resulted in development of a primitive vessel network in the constructs. The modular approach allows microtissues to be separately precultured to harness the dual differentiation potential of MSC to support both bone and blood vessel formation in a unified construct. John Wiley and Sons Inc. 2022-08-12 2022-11 /pmc/articles/PMC9547967/ /pubmed/35922969 http://dx.doi.org/10.1002/bit.28201 Text en © 2022 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle ARTICLES
Schott, Nicholas G.
Vu, Huy
Stegemann, Jan P.
Multimodular vascularized bone construct comprised of vasculogenic and osteogenic microtissues
title Multimodular vascularized bone construct comprised of vasculogenic and osteogenic microtissues
title_full Multimodular vascularized bone construct comprised of vasculogenic and osteogenic microtissues
title_fullStr Multimodular vascularized bone construct comprised of vasculogenic and osteogenic microtissues
title_full_unstemmed Multimodular vascularized bone construct comprised of vasculogenic and osteogenic microtissues
title_short Multimodular vascularized bone construct comprised of vasculogenic and osteogenic microtissues
title_sort multimodular vascularized bone construct comprised of vasculogenic and osteogenic microtissues
topic ARTICLES
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9547967/
https://www.ncbi.nlm.nih.gov/pubmed/35922969
http://dx.doi.org/10.1002/bit.28201
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