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Efficacy of Syzygium aromaticum essential oil on the growth and enzymatic activity of pathogenic Candida albicans strains
BACKGROUND AND PURPOSE: Candida albicans (C. albicans) is the most common human pathogen owing to the most virulence factors. It seems that extracellular hydrolytic enzymes play a key role in C. albicans pathogenicity. The present study aimed to assess the susceptibility and enzymatic activity of pa...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Iranian Society of Medical Mycology
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9548078/ https://www.ncbi.nlm.nih.gov/pubmed/36340432 http://dx.doi.org/10.18502/cmm.8.1.9209 |
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author | Hekmatpanah, Ashkan Sharifzadeh, Aghil Shokri, Hojjatollah abbaszadeh, Sepideh Nikaein, Donya |
author_facet | Hekmatpanah, Ashkan Sharifzadeh, Aghil Shokri, Hojjatollah abbaszadeh, Sepideh Nikaein, Donya |
author_sort | Hekmatpanah, Ashkan |
collection | PubMed |
description | BACKGROUND AND PURPOSE: Candida albicans (C. albicans) is the most common human pathogen owing to the most virulence factors. It seems that extracellular hydrolytic enzymes play a key role in C. albicans pathogenicity. The present study aimed to assess the susceptibility and enzymatic activity of pathogenic C. albicans isolates exposed to the Syzygium aromaticum (S. aromaticum) essential oil. MATERIALS AND METHODS: S. aromaticum oil was characterized using gas chromatography-mass spectrometry (GC–MS). The broth microdilution technique (CLSI, M27-A3) was used to determine the minimum inhibitory concentration (MIC) of test compounds. Furthermore, before and after treatment with S. aromaticum essential oil, the yeasts were analyzed regarding the proteinase (Prz), hemolysin (Hz), and phospholipase (Phz) production/activity. RESULTS: β-caryophyllene (12.76%) was found to be the major constituent in the essential oil after eugenol (84.64%). Only one isolate of C. albicans showed the antifungal resistance to fluconazole. All isolates were susceptible to S. aromaticum essential oil with MIC of 625-1250 μg/ml. S. aromaticum oil represented the best antifungal effect against C. albicans at MIC 1000 μg/ml. The mean±SD enzyme activity of C. albicans not exposed to S. aromaticum essential oil was obtained at 0.55±0.03, 0.73±0.04, and 0.61±0.05 for proteinase, hemolysin, and phospholipase, respectively. The activities of these enzymes were reduced significantly (P<0.05) to 0.33±0.06, 0.40±0.04, and 0.16±0.03 for phospholipase, proteinase, and hemolysin, respectively, after the yeasts were subjected to S. aromaticum essential oil. CONCLUSION: The present study aimed to determine the ability of S. aromaticum essential oil to prevent the growth of C. albicans and decrease their enzymatic activity. As a natural antifungal agent, S. aromaticum can be utilized in pharmaceutical systems. |
format | Online Article Text |
id | pubmed-9548078 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Iranian Society of Medical Mycology |
record_format | MEDLINE/PubMed |
spelling | pubmed-95480782022-11-03 Efficacy of Syzygium aromaticum essential oil on the growth and enzymatic activity of pathogenic Candida albicans strains Hekmatpanah, Ashkan Sharifzadeh, Aghil Shokri, Hojjatollah abbaszadeh, Sepideh Nikaein, Donya Curr Med Mycol Original Article BACKGROUND AND PURPOSE: Candida albicans (C. albicans) is the most common human pathogen owing to the most virulence factors. It seems that extracellular hydrolytic enzymes play a key role in C. albicans pathogenicity. The present study aimed to assess the susceptibility and enzymatic activity of pathogenic C. albicans isolates exposed to the Syzygium aromaticum (S. aromaticum) essential oil. MATERIALS AND METHODS: S. aromaticum oil was characterized using gas chromatography-mass spectrometry (GC–MS). The broth microdilution technique (CLSI, M27-A3) was used to determine the minimum inhibitory concentration (MIC) of test compounds. Furthermore, before and after treatment with S. aromaticum essential oil, the yeasts were analyzed regarding the proteinase (Prz), hemolysin (Hz), and phospholipase (Phz) production/activity. RESULTS: β-caryophyllene (12.76%) was found to be the major constituent in the essential oil after eugenol (84.64%). Only one isolate of C. albicans showed the antifungal resistance to fluconazole. All isolates were susceptible to S. aromaticum essential oil with MIC of 625-1250 μg/ml. S. aromaticum oil represented the best antifungal effect against C. albicans at MIC 1000 μg/ml. The mean±SD enzyme activity of C. albicans not exposed to S. aromaticum essential oil was obtained at 0.55±0.03, 0.73±0.04, and 0.61±0.05 for proteinase, hemolysin, and phospholipase, respectively. The activities of these enzymes were reduced significantly (P<0.05) to 0.33±0.06, 0.40±0.04, and 0.16±0.03 for phospholipase, proteinase, and hemolysin, respectively, after the yeasts were subjected to S. aromaticum essential oil. CONCLUSION: The present study aimed to determine the ability of S. aromaticum essential oil to prevent the growth of C. albicans and decrease their enzymatic activity. As a natural antifungal agent, S. aromaticum can be utilized in pharmaceutical systems. Iranian Society of Medical Mycology 2022-03 /pmc/articles/PMC9548078/ /pubmed/36340432 http://dx.doi.org/10.18502/cmm.8.1.9209 Text en Copyright: © 2021, Published by Mazandaran University of Medical Sciences on behalf of Iranian Society of Medical Mycology and Invasive Fungi Research Center. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY) License ( http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ) which permits unrestricted use, distribution and reproduction in any medium, provided appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Hekmatpanah, Ashkan Sharifzadeh, Aghil Shokri, Hojjatollah abbaszadeh, Sepideh Nikaein, Donya Efficacy of Syzygium aromaticum essential oil on the growth and enzymatic activity of pathogenic Candida albicans strains |
title | Efficacy of Syzygium aromaticum essential oil on the growth and enzymatic activity of pathogenic Candida albicans strains |
title_full | Efficacy of Syzygium aromaticum essential oil on the growth and enzymatic activity of pathogenic Candida albicans strains |
title_fullStr | Efficacy of Syzygium aromaticum essential oil on the growth and enzymatic activity of pathogenic Candida albicans strains |
title_full_unstemmed | Efficacy of Syzygium aromaticum essential oil on the growth and enzymatic activity of pathogenic Candida albicans strains |
title_short | Efficacy of Syzygium aromaticum essential oil on the growth and enzymatic activity of pathogenic Candida albicans strains |
title_sort | efficacy of syzygium aromaticum essential oil on the growth and enzymatic activity of pathogenic candida albicans strains |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9548078/ https://www.ncbi.nlm.nih.gov/pubmed/36340432 http://dx.doi.org/10.18502/cmm.8.1.9209 |
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