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Diversity and antibacterial activity of endophytic bacteria associated with medicinal plant, Scrophularia striata

To search endophytic bacteria diversity and evaluate their antibacterial activity, healthy medicinal plant of Scrophularia striata was chosen in this study. One hundred endophytic bacteria were isolated from surface-sterilized tissues (root, stem and leaf) of S. striata. Using sequence analysis targ...

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Autores principales: Tavarideh, Farhad, Pourahmad, Fazel, Nemati, Mostafa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Urmia University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9548236/
https://www.ncbi.nlm.nih.gov/pubmed/36320307
http://dx.doi.org/10.30466/vrf.2021.529714.3174
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author Tavarideh, Farhad
Pourahmad, Fazel
Nemati, Mostafa
author_facet Tavarideh, Farhad
Pourahmad, Fazel
Nemati, Mostafa
author_sort Tavarideh, Farhad
collection PubMed
description To search endophytic bacteria diversity and evaluate their antibacterial activity, healthy medicinal plant of Scrophularia striata was chosen in this study. One hundred endophytic bacteria were isolated from surface-sterilized tissues (root, stem and leaf) of S. striata. Using sequence analysis targeting 16S rRNA gene, eight genera, including Agrococcus, Arthrobacter, Bacillus, Chryseobacterium, Delftia, Kocuria, Pseudomonas and Sphingomonas were identified. Antibacterial activity of endophytic bacteria was examined against some test bacteria, employing agar well diffusion method. Out of 31 endophytic bacterial isolates, 24(77.42%) isolates showed significant antimicrobial activity against Bacillus cereus, 17(54.84%) isolates exhibited maximum activity against Staphylococcus aureus, 14(45.16%) isolates against Escherichia coli and 5(16.13%) isolates showed positive activity against Proteus mirabilis.The results obtained in this study suggested that the medicinal plant, S. striatais is a potent source of endophytic bacteria with antibacterial activity and offers promise for discovery of more impressive biological compounds.
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spelling pubmed-95482362022-10-31 Diversity and antibacterial activity of endophytic bacteria associated with medicinal plant, Scrophularia striata Tavarideh, Farhad Pourahmad, Fazel Nemati, Mostafa Vet Res Forum Original Article To search endophytic bacteria diversity and evaluate their antibacterial activity, healthy medicinal plant of Scrophularia striata was chosen in this study. One hundred endophytic bacteria were isolated from surface-sterilized tissues (root, stem and leaf) of S. striata. Using sequence analysis targeting 16S rRNA gene, eight genera, including Agrococcus, Arthrobacter, Bacillus, Chryseobacterium, Delftia, Kocuria, Pseudomonas and Sphingomonas were identified. Antibacterial activity of endophytic bacteria was examined against some test bacteria, employing agar well diffusion method. Out of 31 endophytic bacterial isolates, 24(77.42%) isolates showed significant antimicrobial activity against Bacillus cereus, 17(54.84%) isolates exhibited maximum activity against Staphylococcus aureus, 14(45.16%) isolates against Escherichia coli and 5(16.13%) isolates showed positive activity against Proteus mirabilis.The results obtained in this study suggested that the medicinal plant, S. striatais is a potent source of endophytic bacteria with antibacterial activity and offers promise for discovery of more impressive biological compounds. Urmia University Press 2022-09 2022-09-15 /pmc/articles/PMC9548236/ /pubmed/36320307 http://dx.doi.org/10.30466/vrf.2021.529714.3174 Text en © 2022 Urmia University https://creativecommons.org/licenses/by-nc-sa/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.https://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Original Article
Tavarideh, Farhad
Pourahmad, Fazel
Nemati, Mostafa
Diversity and antibacterial activity of endophytic bacteria associated with medicinal plant, Scrophularia striata
title Diversity and antibacterial activity of endophytic bacteria associated with medicinal plant, Scrophularia striata
title_full Diversity and antibacterial activity of endophytic bacteria associated with medicinal plant, Scrophularia striata
title_fullStr Diversity and antibacterial activity of endophytic bacteria associated with medicinal plant, Scrophularia striata
title_full_unstemmed Diversity and antibacterial activity of endophytic bacteria associated with medicinal plant, Scrophularia striata
title_short Diversity and antibacterial activity of endophytic bacteria associated with medicinal plant, Scrophularia striata
title_sort diversity and antibacterial activity of endophytic bacteria associated with medicinal plant, scrophularia striata
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9548236/
https://www.ncbi.nlm.nih.gov/pubmed/36320307
http://dx.doi.org/10.30466/vrf.2021.529714.3174
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