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Single-cell sequencing of PBMC characterizes the altered transcriptomic landscape of classical monocytes in BNT162b2-induced myocarditis

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been the most dangerous threat to public health worldwide for the last few years, which led to the development of the novel mRNA vaccine (BNT162b2). However, BNT162b2 vaccination is known to be associated with myocarditis. Here, as...

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Autores principales: Hwang, Nahee, Huh, Yune, Bu, Seonghyeon, Seo, Kyung Jin, Kwon, Se Hwan, Kim, Jae-woo, Yoon, Bo Kyung, Ahn, Hyo-Suk, Fang, Sungsoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549039/
https://www.ncbi.nlm.nih.gov/pubmed/36225942
http://dx.doi.org/10.3389/fimmu.2022.979188
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author Hwang, Nahee
Huh, Yune
Bu, Seonghyeon
Seo, Kyung Jin
Kwon, Se Hwan
Kim, Jae-woo
Yoon, Bo Kyung
Ahn, Hyo-Suk
Fang, Sungsoon
author_facet Hwang, Nahee
Huh, Yune
Bu, Seonghyeon
Seo, Kyung Jin
Kwon, Se Hwan
Kim, Jae-woo
Yoon, Bo Kyung
Ahn, Hyo-Suk
Fang, Sungsoon
author_sort Hwang, Nahee
collection PubMed
description The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been the most dangerous threat to public health worldwide for the last few years, which led to the development of the novel mRNA vaccine (BNT162b2). However, BNT162b2 vaccination is known to be associated with myocarditis. Here, as an attempt to determine the pathogenesis of the disease and to develop biomarkers to determine whether subjects likely proceed to myocarditis after vaccination, we conducted a time series analysis of peripheral blood mononuclear cells of a patient with BNT162b2-induced myocarditis. Single-cell RNA sequence analysis identified monocytes as the cell clusters with the most dynamic changes. To identify distinct gene expression signatures, we compared monocytes of BNT162b2-induced myocarditis with monocytes under various conditions, including SARS-CoV-2 infection, BNT162b2 vaccination, and Kawasaki disease, a disease similar to myocarditis. Representative changes in the transcriptomic profile of classical monocytes include the upregulation of genes related to fatty acid metabolism and downregulation of transcription factor AP-1 activity. This study provides, for the first time, the importance of classical monocytes in the pathogenesis of myocarditis following BNT162b2 vaccination and presents the possibility that vaccination affects monocytes, further inducing their differentiation and infiltration into the heart.
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spelling pubmed-95490392022-10-11 Single-cell sequencing of PBMC characterizes the altered transcriptomic landscape of classical monocytes in BNT162b2-induced myocarditis Hwang, Nahee Huh, Yune Bu, Seonghyeon Seo, Kyung Jin Kwon, Se Hwan Kim, Jae-woo Yoon, Bo Kyung Ahn, Hyo-Suk Fang, Sungsoon Front Immunol Immunology The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been the most dangerous threat to public health worldwide for the last few years, which led to the development of the novel mRNA vaccine (BNT162b2). However, BNT162b2 vaccination is known to be associated with myocarditis. Here, as an attempt to determine the pathogenesis of the disease and to develop biomarkers to determine whether subjects likely proceed to myocarditis after vaccination, we conducted a time series analysis of peripheral blood mononuclear cells of a patient with BNT162b2-induced myocarditis. Single-cell RNA sequence analysis identified monocytes as the cell clusters with the most dynamic changes. To identify distinct gene expression signatures, we compared monocytes of BNT162b2-induced myocarditis with monocytes under various conditions, including SARS-CoV-2 infection, BNT162b2 vaccination, and Kawasaki disease, a disease similar to myocarditis. Representative changes in the transcriptomic profile of classical monocytes include the upregulation of genes related to fatty acid metabolism and downregulation of transcription factor AP-1 activity. This study provides, for the first time, the importance of classical monocytes in the pathogenesis of myocarditis following BNT162b2 vaccination and presents the possibility that vaccination affects monocytes, further inducing their differentiation and infiltration into the heart. Frontiers Media S.A. 2022-09-26 /pmc/articles/PMC9549039/ /pubmed/36225942 http://dx.doi.org/10.3389/fimmu.2022.979188 Text en Copyright © 2022 Hwang, Huh, Bu, Seo, Kwon, Kim, Yoon, Ahn and Fang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Hwang, Nahee
Huh, Yune
Bu, Seonghyeon
Seo, Kyung Jin
Kwon, Se Hwan
Kim, Jae-woo
Yoon, Bo Kyung
Ahn, Hyo-Suk
Fang, Sungsoon
Single-cell sequencing of PBMC characterizes the altered transcriptomic landscape of classical monocytes in BNT162b2-induced myocarditis
title Single-cell sequencing of PBMC characterizes the altered transcriptomic landscape of classical monocytes in BNT162b2-induced myocarditis
title_full Single-cell sequencing of PBMC characterizes the altered transcriptomic landscape of classical monocytes in BNT162b2-induced myocarditis
title_fullStr Single-cell sequencing of PBMC characterizes the altered transcriptomic landscape of classical monocytes in BNT162b2-induced myocarditis
title_full_unstemmed Single-cell sequencing of PBMC characterizes the altered transcriptomic landscape of classical monocytes in BNT162b2-induced myocarditis
title_short Single-cell sequencing of PBMC characterizes the altered transcriptomic landscape of classical monocytes in BNT162b2-induced myocarditis
title_sort single-cell sequencing of pbmc characterizes the altered transcriptomic landscape of classical monocytes in bnt162b2-induced myocarditis
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549039/
https://www.ncbi.nlm.nih.gov/pubmed/36225942
http://dx.doi.org/10.3389/fimmu.2022.979188
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