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The ghrelin system follows a precise post-natal development in mini-pigs that is not impacted by dietary medium chain fatty-acids

The ghrelin-ghrelin receptor (GHSR1) system is one of the most important mechanisms regulating food intake and energy balance. To be fully active, ghrelin is acylated with medium-chain fatty acids (MCFA) through the ghrelin-O-acetyl transferase (GOAT). Several studies reported an impact of dietary M...

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Autores principales: Boudry, Gaëlle, Cahu, Armelle, Romé, Véronique, Janvier, Régis, Louvois, Margaux, Catheline, Daniel, Rioux, Vincent, Le Huërou-Luron, Isabelle, Blat, Sophie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549131/
https://www.ncbi.nlm.nih.gov/pubmed/36225304
http://dx.doi.org/10.3389/fphys.2022.1010586
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author Boudry, Gaëlle
Cahu, Armelle
Romé, Véronique
Janvier, Régis
Louvois, Margaux
Catheline, Daniel
Rioux, Vincent
Le Huërou-Luron, Isabelle
Blat, Sophie
author_facet Boudry, Gaëlle
Cahu, Armelle
Romé, Véronique
Janvier, Régis
Louvois, Margaux
Catheline, Daniel
Rioux, Vincent
Le Huërou-Luron, Isabelle
Blat, Sophie
author_sort Boudry, Gaëlle
collection PubMed
description The ghrelin-ghrelin receptor (GHSR1) system is one of the most important mechanisms regulating food intake and energy balance. To be fully active, ghrelin is acylated with medium-chain fatty acids (MCFA) through the ghrelin-O-acetyl transferase (GOAT). Several studies reported an impact of dietary MCFA on ghrelin acylation in adults. Our study aimed at describing early post-natal development of the ghrelin system in mini-pigs as a model of human neonates and evaluating the impact of dietary MCFA. Suckled mini-pigs were sacrificed at post-natal day (PND) 0, 2, 5, and 10 or at adult stage. In parallel, other mini-pigs were fed from birth to PND10 a standard or a dairy lipid-enriched formula with increased MCFA concentration (DL-IF). Plasma ghrelin transiently peaked at PND2, with no variation of the acylated fraction except in adults where it was greater than during the neonatal period. Levels of mRNA coding pre-proghrelin (GHRL) and GOAT in the antrum did not vary during the post-natal period but dropped in adults. Levels of antral pcsk1/3 (cleaving GHRL into ghrelin) mRNA decreased significantly with age and was negatively correlated with plasma acylated, but not total, ghrelin. Hypothalamic ghsr1 mRNA did not vary in neonates but increased in adults. The DL-IF formula enriched antral tissue with MCFA but did not impact the ghrelin system. In conclusion, the ghrelin maturation enzyme PCSK1/3 gene expression exhibited post-natal modifications parallel to transient variations in circulating plasma ghrelin level in suckling piglets but dietary MCFA did not impact this post-natal development.
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spelling pubmed-95491312022-10-11 The ghrelin system follows a precise post-natal development in mini-pigs that is not impacted by dietary medium chain fatty-acids Boudry, Gaëlle Cahu, Armelle Romé, Véronique Janvier, Régis Louvois, Margaux Catheline, Daniel Rioux, Vincent Le Huërou-Luron, Isabelle Blat, Sophie Front Physiol Physiology The ghrelin-ghrelin receptor (GHSR1) system is one of the most important mechanisms regulating food intake and energy balance. To be fully active, ghrelin is acylated with medium-chain fatty acids (MCFA) through the ghrelin-O-acetyl transferase (GOAT). Several studies reported an impact of dietary MCFA on ghrelin acylation in adults. Our study aimed at describing early post-natal development of the ghrelin system in mini-pigs as a model of human neonates and evaluating the impact of dietary MCFA. Suckled mini-pigs were sacrificed at post-natal day (PND) 0, 2, 5, and 10 or at adult stage. In parallel, other mini-pigs were fed from birth to PND10 a standard or a dairy lipid-enriched formula with increased MCFA concentration (DL-IF). Plasma ghrelin transiently peaked at PND2, with no variation of the acylated fraction except in adults where it was greater than during the neonatal period. Levels of mRNA coding pre-proghrelin (GHRL) and GOAT in the antrum did not vary during the post-natal period but dropped in adults. Levels of antral pcsk1/3 (cleaving GHRL into ghrelin) mRNA decreased significantly with age and was negatively correlated with plasma acylated, but not total, ghrelin. Hypothalamic ghsr1 mRNA did not vary in neonates but increased in adults. The DL-IF formula enriched antral tissue with MCFA but did not impact the ghrelin system. In conclusion, the ghrelin maturation enzyme PCSK1/3 gene expression exhibited post-natal modifications parallel to transient variations in circulating plasma ghrelin level in suckling piglets but dietary MCFA did not impact this post-natal development. Frontiers Media S.A. 2022-09-26 /pmc/articles/PMC9549131/ /pubmed/36225304 http://dx.doi.org/10.3389/fphys.2022.1010586 Text en Copyright © 2022 Boudry, Cahu, Romé, Janvier, Louvois, Catheline, Rioux, Le Huërou-Luron and Blat. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Boudry, Gaëlle
Cahu, Armelle
Romé, Véronique
Janvier, Régis
Louvois, Margaux
Catheline, Daniel
Rioux, Vincent
Le Huërou-Luron, Isabelle
Blat, Sophie
The ghrelin system follows a precise post-natal development in mini-pigs that is not impacted by dietary medium chain fatty-acids
title The ghrelin system follows a precise post-natal development in mini-pigs that is not impacted by dietary medium chain fatty-acids
title_full The ghrelin system follows a precise post-natal development in mini-pigs that is not impacted by dietary medium chain fatty-acids
title_fullStr The ghrelin system follows a precise post-natal development in mini-pigs that is not impacted by dietary medium chain fatty-acids
title_full_unstemmed The ghrelin system follows a precise post-natal development in mini-pigs that is not impacted by dietary medium chain fatty-acids
title_short The ghrelin system follows a precise post-natal development in mini-pigs that is not impacted by dietary medium chain fatty-acids
title_sort ghrelin system follows a precise post-natal development in mini-pigs that is not impacted by dietary medium chain fatty-acids
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549131/
https://www.ncbi.nlm.nih.gov/pubmed/36225304
http://dx.doi.org/10.3389/fphys.2022.1010586
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