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Phylogenetic survey of the subtilase family and a data-mining-based search for new subtilisins from Bacillaceae

The subtilase family (S8), a member of the clan SB of serine proteases are ubiquitous in all kingdoms of life and fulfil different physiological functions. Subtilases are divided in several groups and especially subtilisins are of interest as they are used in various industrial sectors. Therefore, w...

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Autores principales: Falkenberg, Fabian, Bott, Michael, Bongaerts, Johannes, Siegert, Petra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549277/
https://www.ncbi.nlm.nih.gov/pubmed/36225363
http://dx.doi.org/10.3389/fmicb.2022.1017978
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author Falkenberg, Fabian
Bott, Michael
Bongaerts, Johannes
Siegert, Petra
author_facet Falkenberg, Fabian
Bott, Michael
Bongaerts, Johannes
Siegert, Petra
author_sort Falkenberg, Fabian
collection PubMed
description The subtilase family (S8), a member of the clan SB of serine proteases are ubiquitous in all kingdoms of life and fulfil different physiological functions. Subtilases are divided in several groups and especially subtilisins are of interest as they are used in various industrial sectors. Therefore, we searched for new subtilisin sequences of the family Bacillaceae using a data mining approach. The obtained 1,400 sequences were phylogenetically classified in the context of the subtilase family. This required an updated comprehensive overview of the different groups within this family. To fill this gap, we conducted a phylogenetic survey of the S8 family with characterised holotypes derived from the MEROPS database. The analysis revealed the presence of eight previously uncharacterised groups and 13 subgroups within the S8 family. The sequences that emerged from the data mining with the set filter parameters were mainly assigned to the subtilisin subgroups of true subtilisins, high-alkaline subtilisins, and phylogenetically intermediate subtilisins and represent an excellent source for new subtilisin candidates.
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spelling pubmed-95492772022-10-11 Phylogenetic survey of the subtilase family and a data-mining-based search for new subtilisins from Bacillaceae Falkenberg, Fabian Bott, Michael Bongaerts, Johannes Siegert, Petra Front Microbiol Microbiology The subtilase family (S8), a member of the clan SB of serine proteases are ubiquitous in all kingdoms of life and fulfil different physiological functions. Subtilases are divided in several groups and especially subtilisins are of interest as they are used in various industrial sectors. Therefore, we searched for new subtilisin sequences of the family Bacillaceae using a data mining approach. The obtained 1,400 sequences were phylogenetically classified in the context of the subtilase family. This required an updated comprehensive overview of the different groups within this family. To fill this gap, we conducted a phylogenetic survey of the S8 family with characterised holotypes derived from the MEROPS database. The analysis revealed the presence of eight previously uncharacterised groups and 13 subgroups within the S8 family. The sequences that emerged from the data mining with the set filter parameters were mainly assigned to the subtilisin subgroups of true subtilisins, high-alkaline subtilisins, and phylogenetically intermediate subtilisins and represent an excellent source for new subtilisin candidates. Frontiers Media S.A. 2022-09-26 /pmc/articles/PMC9549277/ /pubmed/36225363 http://dx.doi.org/10.3389/fmicb.2022.1017978 Text en Copyright © 2022 Falkenberg, Bott, Bongaerts and Siegert. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Falkenberg, Fabian
Bott, Michael
Bongaerts, Johannes
Siegert, Petra
Phylogenetic survey of the subtilase family and a data-mining-based search for new subtilisins from Bacillaceae
title Phylogenetic survey of the subtilase family and a data-mining-based search for new subtilisins from Bacillaceae
title_full Phylogenetic survey of the subtilase family and a data-mining-based search for new subtilisins from Bacillaceae
title_fullStr Phylogenetic survey of the subtilase family and a data-mining-based search for new subtilisins from Bacillaceae
title_full_unstemmed Phylogenetic survey of the subtilase family and a data-mining-based search for new subtilisins from Bacillaceae
title_short Phylogenetic survey of the subtilase family and a data-mining-based search for new subtilisins from Bacillaceae
title_sort phylogenetic survey of the subtilase family and a data-mining-based search for new subtilisins from bacillaceae
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549277/
https://www.ncbi.nlm.nih.gov/pubmed/36225363
http://dx.doi.org/10.3389/fmicb.2022.1017978
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