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Isolation and characterization of phosphate solubilizing bacteria naturally colonizing legumes rhizosphere in Morocco
Low-cost and environmentally friendly agricultural practices have received increasing attention in recent years. Developing microbial inoculants containing phosphate (P) solubilizing bacteria (PSB) represents an emerging biological solution to improve rhizosphere P availability. The present study ai...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549286/ https://www.ncbi.nlm.nih.gov/pubmed/36225374 http://dx.doi.org/10.3389/fmicb.2022.958300 |
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author | Janati, Walid Mikou, Karima El Ghadraoui, Lahsen Errachidi, Faouzi |
author_facet | Janati, Walid Mikou, Karima El Ghadraoui, Lahsen Errachidi, Faouzi |
author_sort | Janati, Walid |
collection | PubMed |
description | Low-cost and environmentally friendly agricultural practices have received increasing attention in recent years. Developing microbial inoculants containing phosphate (P) solubilizing bacteria (PSB) represents an emerging biological solution to improve rhizosphere P availability. The present study aims to explore PSB strains isolated from soils located at different bioclimatic stages in Morocco and present in various legumes rhizosphere to improve agronomic microbial fertilizer’s effectiveness. It was also aimed to test the isolated strains for their ability to solubilize P in NBRIP medium with Tricalcium P (Ca(3) (PO(4))(2)) (TCP), rock phosphate (RP), and their combination as a source of phosphorus, by (2(2)) experiment design. Bacterial strains with a high P solubility index (PSI) were selected, characterized, and compared to commercial control. The vanadate-molybdate method was used to estimate P solubilization activity. Stress tolerance to salinity, acidity, drought, and temperature was tested. From all isolated strains (64), 12 were screened as promising biotechnological interest because of their P solubilization and their good resistance to different drastic conditions. Besides, the strain WJEF15 showed the most P solubility efficiency in NBRIP solid medium with a PSI of 4.1; while the WJEF61 strain was located as the most efficient strain in NBRIP-TCP liquid medium by releasing 147.62 mg.l(–1) of soluble P. In contrast, in the NBRIP-RP medium, the strain WJEF15 presented maximum solubilization with 25.16 mg.l(–1). The experiment design showed that a combination of RP and TCP with max level progressively increases P solubilization by 20.58%, while the WJEF63 strain has the most efficient concentration of 102.69 mg.l(–1). Indeed, among the selected strains, four strains were able to limit tested fungi growth. Thus, results reveal a potential effect of selecting PSBs to support cropping cultures as plant growth-promoting rhizobacteria (PGPR). |
format | Online Article Text |
id | pubmed-9549286 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95492862022-10-11 Isolation and characterization of phosphate solubilizing bacteria naturally colonizing legumes rhizosphere in Morocco Janati, Walid Mikou, Karima El Ghadraoui, Lahsen Errachidi, Faouzi Front Microbiol Microbiology Low-cost and environmentally friendly agricultural practices have received increasing attention in recent years. Developing microbial inoculants containing phosphate (P) solubilizing bacteria (PSB) represents an emerging biological solution to improve rhizosphere P availability. The present study aims to explore PSB strains isolated from soils located at different bioclimatic stages in Morocco and present in various legumes rhizosphere to improve agronomic microbial fertilizer’s effectiveness. It was also aimed to test the isolated strains for their ability to solubilize P in NBRIP medium with Tricalcium P (Ca(3) (PO(4))(2)) (TCP), rock phosphate (RP), and their combination as a source of phosphorus, by (2(2)) experiment design. Bacterial strains with a high P solubility index (PSI) were selected, characterized, and compared to commercial control. The vanadate-molybdate method was used to estimate P solubilization activity. Stress tolerance to salinity, acidity, drought, and temperature was tested. From all isolated strains (64), 12 were screened as promising biotechnological interest because of their P solubilization and their good resistance to different drastic conditions. Besides, the strain WJEF15 showed the most P solubility efficiency in NBRIP solid medium with a PSI of 4.1; while the WJEF61 strain was located as the most efficient strain in NBRIP-TCP liquid medium by releasing 147.62 mg.l(–1) of soluble P. In contrast, in the NBRIP-RP medium, the strain WJEF15 presented maximum solubilization with 25.16 mg.l(–1). The experiment design showed that a combination of RP and TCP with max level progressively increases P solubilization by 20.58%, while the WJEF63 strain has the most efficient concentration of 102.69 mg.l(–1). Indeed, among the selected strains, four strains were able to limit tested fungi growth. Thus, results reveal a potential effect of selecting PSBs to support cropping cultures as plant growth-promoting rhizobacteria (PGPR). Frontiers Media S.A. 2022-09-26 /pmc/articles/PMC9549286/ /pubmed/36225374 http://dx.doi.org/10.3389/fmicb.2022.958300 Text en Copyright © 2022 Janati, Mikou, El Ghadraoui and Errachidi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Janati, Walid Mikou, Karima El Ghadraoui, Lahsen Errachidi, Faouzi Isolation and characterization of phosphate solubilizing bacteria naturally colonizing legumes rhizosphere in Morocco |
title | Isolation and characterization of phosphate solubilizing bacteria naturally colonizing legumes rhizosphere in Morocco |
title_full | Isolation and characterization of phosphate solubilizing bacteria naturally colonizing legumes rhizosphere in Morocco |
title_fullStr | Isolation and characterization of phosphate solubilizing bacteria naturally colonizing legumes rhizosphere in Morocco |
title_full_unstemmed | Isolation and characterization of phosphate solubilizing bacteria naturally colonizing legumes rhizosphere in Morocco |
title_short | Isolation and characterization of phosphate solubilizing bacteria naturally colonizing legumes rhizosphere in Morocco |
title_sort | isolation and characterization of phosphate solubilizing bacteria naturally colonizing legumes rhizosphere in morocco |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549286/ https://www.ncbi.nlm.nih.gov/pubmed/36225374 http://dx.doi.org/10.3389/fmicb.2022.958300 |
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