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Extracellular self-RNA: A danger elicitor in pepper induces immunity against bacterial and viral pathogens in the field

Plants and animals serve as hosts for microbes. To protect themselves from microbe-induced damage, plants and animals need to differentiate self-molecules/signals from non-self, microbe-derived molecules. Damage-associated molecular patterns (DAMPs) are danger signals released from the damaged host...

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Autores principales: Kim, Doyeon, Riu, Myoungjoo, Oh, Sang-Keun, Ryu, Choong-Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549290/
https://www.ncbi.nlm.nih.gov/pubmed/36226289
http://dx.doi.org/10.3389/fpls.2022.864086
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author Kim, Doyeon
Riu, Myoungjoo
Oh, Sang-Keun
Ryu, Choong-Min
author_facet Kim, Doyeon
Riu, Myoungjoo
Oh, Sang-Keun
Ryu, Choong-Min
author_sort Kim, Doyeon
collection PubMed
description Plants and animals serve as hosts for microbes. To protect themselves from microbe-induced damage, plants and animals need to differentiate self-molecules/signals from non-self, microbe-derived molecules. Damage-associated molecular patterns (DAMPs) are danger signals released from the damaged host tissue or present on the surface of stressed cells. Although a self-extracellular DNA has previously been shown to act as a DAMP in different plant species, the existence of a self-extracellular RNA (eRNA) as a danger signal in plants remains unknown. Here, we firstly evaluated the ability of a pepper self-eRNA to activate immunity against viral and bacterial pathogens under field conditions. Pepper leaves pre-infiltrated with self-eRNA exhibited reduced titer of the naturally occurring Tomato spotted wilt virus and diminished symptoms of Xanthomonas axonopodis pv. vesicatoria infection through eliciting defense priming of abscisic acid signaling. At the end of the growing season at 90 days after transplanting, pepper plants treated with self- and non-self-eRNAs showed no difference in fruit yield. Taken together, our discovery demonstrated that self-eRNA can successfully activate plant systemic immunity without any growth penalty, indicating its potential as a novel disease management agent against a broad range of pathogenic microbes.
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spelling pubmed-95492902022-10-11 Extracellular self-RNA: A danger elicitor in pepper induces immunity against bacterial and viral pathogens in the field Kim, Doyeon Riu, Myoungjoo Oh, Sang-Keun Ryu, Choong-Min Front Plant Sci Plant Science Plants and animals serve as hosts for microbes. To protect themselves from microbe-induced damage, plants and animals need to differentiate self-molecules/signals from non-self, microbe-derived molecules. Damage-associated molecular patterns (DAMPs) are danger signals released from the damaged host tissue or present on the surface of stressed cells. Although a self-extracellular DNA has previously been shown to act as a DAMP in different plant species, the existence of a self-extracellular RNA (eRNA) as a danger signal in plants remains unknown. Here, we firstly evaluated the ability of a pepper self-eRNA to activate immunity against viral and bacterial pathogens under field conditions. Pepper leaves pre-infiltrated with self-eRNA exhibited reduced titer of the naturally occurring Tomato spotted wilt virus and diminished symptoms of Xanthomonas axonopodis pv. vesicatoria infection through eliciting defense priming of abscisic acid signaling. At the end of the growing season at 90 days after transplanting, pepper plants treated with self- and non-self-eRNAs showed no difference in fruit yield. Taken together, our discovery demonstrated that self-eRNA can successfully activate plant systemic immunity without any growth penalty, indicating its potential as a novel disease management agent against a broad range of pathogenic microbes. Frontiers Media S.A. 2022-09-26 /pmc/articles/PMC9549290/ /pubmed/36226289 http://dx.doi.org/10.3389/fpls.2022.864086 Text en Copyright © 2022 Kim, Riu, Oh and Ryu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Kim, Doyeon
Riu, Myoungjoo
Oh, Sang-Keun
Ryu, Choong-Min
Extracellular self-RNA: A danger elicitor in pepper induces immunity against bacterial and viral pathogens in the field
title Extracellular self-RNA: A danger elicitor in pepper induces immunity against bacterial and viral pathogens in the field
title_full Extracellular self-RNA: A danger elicitor in pepper induces immunity against bacterial and viral pathogens in the field
title_fullStr Extracellular self-RNA: A danger elicitor in pepper induces immunity against bacterial and viral pathogens in the field
title_full_unstemmed Extracellular self-RNA: A danger elicitor in pepper induces immunity against bacterial and viral pathogens in the field
title_short Extracellular self-RNA: A danger elicitor in pepper induces immunity against bacterial and viral pathogens in the field
title_sort extracellular self-rna: a danger elicitor in pepper induces immunity against bacterial and viral pathogens in the field
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549290/
https://www.ncbi.nlm.nih.gov/pubmed/36226289
http://dx.doi.org/10.3389/fpls.2022.864086
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