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Development of a novel cell-based, In-Cell Western/ERK assay system for the high-throughput screening of agonists acting on the delta-opioid receptor

Background: Extracellular signal-regulated kinases (ERKs) are important signaling mediators in mammalian cells and, as a result, one of the major areas of research focus. The detection and quantification of ERK phosphorylation as an index of activation is normally conducted using immunoblotting, whi...

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Detalles Bibliográficos
Autores principales: Asghar, Junaid, Latif, Liaque, Alexander, Stephen P. H., Kendall, David A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549385/
https://www.ncbi.nlm.nih.gov/pubmed/36225576
http://dx.doi.org/10.3389/fphar.2022.933356