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Nucleobindin‐2/nesfatin‐1 enhances the cell proliferation, migration, invasion and epithelial‐mesenchymal transition in gastric carcinoma
Nesfatin‐1, a newly discovered adipokine derived from nucleobindin‐2 (NUCB2), has been described as a new prognostic marker in cancers. This study aimed to explore the functional role of NUCB2/nesfatin‐1 in the cell proliferation, migration and invasion in gastric carcinoma (GC). The expressions of...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549493/ https://www.ncbi.nlm.nih.gov/pubmed/36065769 http://dx.doi.org/10.1111/jcmm.17522 |
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author | Ren, Le Bao, Deming Wang, Liming Xu, Qin Xu, Yayun Shi, Zhenwang |
author_facet | Ren, Le Bao, Deming Wang, Liming Xu, Qin Xu, Yayun Shi, Zhenwang |
author_sort | Ren, Le |
collection | PubMed |
description | Nesfatin‐1, a newly discovered adipokine derived from nucleobindin‐2 (NUCB2), has been described as a new prognostic marker in cancers. This study aimed to explore the functional role of NUCB2/nesfatin‐1 in the cell proliferation, migration and invasion in gastric carcinoma (GC). The expressions of NUCB2/nesfatin‐1 in GC tissues and normal adjacent tissues (NATs) were compared, and the effect of inhibition of NUCB2/nesfatin‐1 on the cell proliferation, migration, invasion and epithelial‐mesenchymal transition (EMT) in GC cell line SGC‐7901 was investigated. Cell transfection was conducted to inhibit NUCB2/nesfatin‐1 by short hairpin RNA. Cell proliferation, migration and invasion abilities were determined using cell counting kit‐8 (CCK‐8), 5‐ethynyl‐2′‐deoxyuridine (EdU), wound healing and transwell assays, respectively. The expressions of EMT markers E‐Cadherin and N‐Cadherin were determined using western blotting. The expression of NUCB2/nesfatin‐1 protein in GC tissues was significantly increased compared with that in NATs. Consistently, the serum concentrations of NUCB2/nesfatin‐1 were significantly higher in patients with GC as compared with those in the control group. Moreover, the results of CCK‐8 assay and EdU assay indicated that knockdown of NUCB2/nesfatin‐1 could markedly decrease SGC‐7901 proliferation. Furthermore, the results of wound healing assay and transwell assay demonstrated that knockdown of NUCB2/nesfatin‐1 significantly suppressed SGC‐7901 migration and invasion abilities. Additionally, knockdown of NUCB2/nesfatin‐1 decreased the expressions of N‐Cadherin and increased the expressions of E‐Cadherin in SGC‐7901 cells. These findings suggest that knockdown of NUCB2/nesfatin‐1 suppressed the proliferation, migration, invasion and EMT of SGC‐7901 cells, suggesting a potentially promising therapeutic target for GC. |
format | Online Article Text |
id | pubmed-9549493 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95494932022-10-14 Nucleobindin‐2/nesfatin‐1 enhances the cell proliferation, migration, invasion and epithelial‐mesenchymal transition in gastric carcinoma Ren, Le Bao, Deming Wang, Liming Xu, Qin Xu, Yayun Shi, Zhenwang J Cell Mol Med Original Articles Nesfatin‐1, a newly discovered adipokine derived from nucleobindin‐2 (NUCB2), has been described as a new prognostic marker in cancers. This study aimed to explore the functional role of NUCB2/nesfatin‐1 in the cell proliferation, migration and invasion in gastric carcinoma (GC). The expressions of NUCB2/nesfatin‐1 in GC tissues and normal adjacent tissues (NATs) were compared, and the effect of inhibition of NUCB2/nesfatin‐1 on the cell proliferation, migration, invasion and epithelial‐mesenchymal transition (EMT) in GC cell line SGC‐7901 was investigated. Cell transfection was conducted to inhibit NUCB2/nesfatin‐1 by short hairpin RNA. Cell proliferation, migration and invasion abilities were determined using cell counting kit‐8 (CCK‐8), 5‐ethynyl‐2′‐deoxyuridine (EdU), wound healing and transwell assays, respectively. The expressions of EMT markers E‐Cadherin and N‐Cadherin were determined using western blotting. The expression of NUCB2/nesfatin‐1 protein in GC tissues was significantly increased compared with that in NATs. Consistently, the serum concentrations of NUCB2/nesfatin‐1 were significantly higher in patients with GC as compared with those in the control group. Moreover, the results of CCK‐8 assay and EdU assay indicated that knockdown of NUCB2/nesfatin‐1 could markedly decrease SGC‐7901 proliferation. Furthermore, the results of wound healing assay and transwell assay demonstrated that knockdown of NUCB2/nesfatin‐1 significantly suppressed SGC‐7901 migration and invasion abilities. Additionally, knockdown of NUCB2/nesfatin‐1 decreased the expressions of N‐Cadherin and increased the expressions of E‐Cadherin in SGC‐7901 cells. These findings suggest that knockdown of NUCB2/nesfatin‐1 suppressed the proliferation, migration, invasion and EMT of SGC‐7901 cells, suggesting a potentially promising therapeutic target for GC. John Wiley and Sons Inc. 2022-09-06 2022-10 /pmc/articles/PMC9549493/ /pubmed/36065769 http://dx.doi.org/10.1111/jcmm.17522 Text en © 2022 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Ren, Le Bao, Deming Wang, Liming Xu, Qin Xu, Yayun Shi, Zhenwang Nucleobindin‐2/nesfatin‐1 enhances the cell proliferation, migration, invasion and epithelial‐mesenchymal transition in gastric carcinoma |
title | Nucleobindin‐2/nesfatin‐1 enhances the cell proliferation, migration, invasion and epithelial‐mesenchymal transition in gastric carcinoma |
title_full | Nucleobindin‐2/nesfatin‐1 enhances the cell proliferation, migration, invasion and epithelial‐mesenchymal transition in gastric carcinoma |
title_fullStr | Nucleobindin‐2/nesfatin‐1 enhances the cell proliferation, migration, invasion and epithelial‐mesenchymal transition in gastric carcinoma |
title_full_unstemmed | Nucleobindin‐2/nesfatin‐1 enhances the cell proliferation, migration, invasion and epithelial‐mesenchymal transition in gastric carcinoma |
title_short | Nucleobindin‐2/nesfatin‐1 enhances the cell proliferation, migration, invasion and epithelial‐mesenchymal transition in gastric carcinoma |
title_sort | nucleobindin‐2/nesfatin‐1 enhances the cell proliferation, migration, invasion and epithelial‐mesenchymal transition in gastric carcinoma |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549493/ https://www.ncbi.nlm.nih.gov/pubmed/36065769 http://dx.doi.org/10.1111/jcmm.17522 |
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