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Assessing and enhancing migration of human myogenic progenitors using directed iPS cell differentiation and advanced tissue modelling
Muscle satellite stem cells (MuSCs) are responsible for skeletal muscle growth and regeneration. Despite their differentiation potential, human MuSCs have limited in vitro expansion and in vivo migration capacity, limiting their use in cell therapies for diseases affecting multiple skeletal muscles....
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549733/ https://www.ncbi.nlm.nih.gov/pubmed/36161772 http://dx.doi.org/10.15252/emmm.202114526 |
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author | Choi, SungWoo Ferrari, Giulia Moyle, Louise A Mackinlay, Kirsty Naouar, Naira Jalal, Salma Benedetti, Sara Wells, Christine Muntoni, Francesco Tedesco, Francesco Saverio |
author_facet | Choi, SungWoo Ferrari, Giulia Moyle, Louise A Mackinlay, Kirsty Naouar, Naira Jalal, Salma Benedetti, Sara Wells, Christine Muntoni, Francesco Tedesco, Francesco Saverio |
author_sort | Choi, SungWoo |
collection | PubMed |
description | Muscle satellite stem cells (MuSCs) are responsible for skeletal muscle growth and regeneration. Despite their differentiation potential, human MuSCs have limited in vitro expansion and in vivo migration capacity, limiting their use in cell therapies for diseases affecting multiple skeletal muscles. Several protocols have been developed to derive MuSC‐like progenitors from human induced pluripotent stem (iPS) cells (hiPSCs) to establish a source of myogenic cells with controllable proliferation and differentiation. However, current hiPSC myogenic derivatives also suffer from limitations of cell migration, ultimately delaying their clinical translation. Here we use a multi‐disciplinary approach including bioinformatics and tissue engineering to show that DLL4 and PDGF‐BB improve migration of hiPSC‐derived myogenic progenitors. Transcriptomic analyses demonstrate that this property is conserved across species and multiple hiPSC lines, consistent with results from single cell motility profiling. Treated cells showed enhanced trans‐endothelial migration in transwell assays. Finally, increased motility was detected in a novel humanised assay to study cell migration using 3D artificial muscles, harnessing advanced tissue modelling to move hiPSCs closer to future muscle gene and cell therapies. |
format | Online Article Text |
id | pubmed-9549733 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95497332022-10-14 Assessing and enhancing migration of human myogenic progenitors using directed iPS cell differentiation and advanced tissue modelling Choi, SungWoo Ferrari, Giulia Moyle, Louise A Mackinlay, Kirsty Naouar, Naira Jalal, Salma Benedetti, Sara Wells, Christine Muntoni, Francesco Tedesco, Francesco Saverio EMBO Mol Med Articles Muscle satellite stem cells (MuSCs) are responsible for skeletal muscle growth and regeneration. Despite their differentiation potential, human MuSCs have limited in vitro expansion and in vivo migration capacity, limiting their use in cell therapies for diseases affecting multiple skeletal muscles. Several protocols have been developed to derive MuSC‐like progenitors from human induced pluripotent stem (iPS) cells (hiPSCs) to establish a source of myogenic cells with controllable proliferation and differentiation. However, current hiPSC myogenic derivatives also suffer from limitations of cell migration, ultimately delaying their clinical translation. Here we use a multi‐disciplinary approach including bioinformatics and tissue engineering to show that DLL4 and PDGF‐BB improve migration of hiPSC‐derived myogenic progenitors. Transcriptomic analyses demonstrate that this property is conserved across species and multiple hiPSC lines, consistent with results from single cell motility profiling. Treated cells showed enhanced trans‐endothelial migration in transwell assays. Finally, increased motility was detected in a novel humanised assay to study cell migration using 3D artificial muscles, harnessing advanced tissue modelling to move hiPSCs closer to future muscle gene and cell therapies. John Wiley and Sons Inc. 2022-09-26 /pmc/articles/PMC9549733/ /pubmed/36161772 http://dx.doi.org/10.15252/emmm.202114526 Text en © 2022 The Authors. Published under the terms of the CC BY 4.0 license. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Choi, SungWoo Ferrari, Giulia Moyle, Louise A Mackinlay, Kirsty Naouar, Naira Jalal, Salma Benedetti, Sara Wells, Christine Muntoni, Francesco Tedesco, Francesco Saverio Assessing and enhancing migration of human myogenic progenitors using directed iPS cell differentiation and advanced tissue modelling |
title | Assessing and enhancing migration of human myogenic progenitors using directed iPS cell differentiation and advanced tissue modelling |
title_full | Assessing and enhancing migration of human myogenic progenitors using directed iPS cell differentiation and advanced tissue modelling |
title_fullStr | Assessing and enhancing migration of human myogenic progenitors using directed iPS cell differentiation and advanced tissue modelling |
title_full_unstemmed | Assessing and enhancing migration of human myogenic progenitors using directed iPS cell differentiation and advanced tissue modelling |
title_short | Assessing and enhancing migration of human myogenic progenitors using directed iPS cell differentiation and advanced tissue modelling |
title_sort | assessing and enhancing migration of human myogenic progenitors using directed ips cell differentiation and advanced tissue modelling |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549733/ https://www.ncbi.nlm.nih.gov/pubmed/36161772 http://dx.doi.org/10.15252/emmm.202114526 |
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