Cargando…

Assessing and enhancing migration of human myogenic progenitors using directed iPS cell differentiation and advanced tissue modelling

Muscle satellite stem cells (MuSCs) are responsible for skeletal muscle growth and regeneration. Despite their differentiation potential, human MuSCs have limited in vitro expansion and in vivo migration capacity, limiting their use in cell therapies for diseases affecting multiple skeletal muscles....

Descripción completa

Detalles Bibliográficos
Autores principales: Choi, SungWoo, Ferrari, Giulia, Moyle, Louise A, Mackinlay, Kirsty, Naouar, Naira, Jalal, Salma, Benedetti, Sara, Wells, Christine, Muntoni, Francesco, Tedesco, Francesco Saverio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549733/
https://www.ncbi.nlm.nih.gov/pubmed/36161772
http://dx.doi.org/10.15252/emmm.202114526
_version_ 1784805735752794112
author Choi, SungWoo
Ferrari, Giulia
Moyle, Louise A
Mackinlay, Kirsty
Naouar, Naira
Jalal, Salma
Benedetti, Sara
Wells, Christine
Muntoni, Francesco
Tedesco, Francesco Saverio
author_facet Choi, SungWoo
Ferrari, Giulia
Moyle, Louise A
Mackinlay, Kirsty
Naouar, Naira
Jalal, Salma
Benedetti, Sara
Wells, Christine
Muntoni, Francesco
Tedesco, Francesco Saverio
author_sort Choi, SungWoo
collection PubMed
description Muscle satellite stem cells (MuSCs) are responsible for skeletal muscle growth and regeneration. Despite their differentiation potential, human MuSCs have limited in vitro expansion and in vivo migration capacity, limiting their use in cell therapies for diseases affecting multiple skeletal muscles. Several protocols have been developed to derive MuSC‐like progenitors from human induced pluripotent stem (iPS) cells (hiPSCs) to establish a source of myogenic cells with controllable proliferation and differentiation. However, current hiPSC myogenic derivatives also suffer from limitations of cell migration, ultimately delaying their clinical translation. Here we use a multi‐disciplinary approach including bioinformatics and tissue engineering to show that DLL4 and PDGF‐BB improve migration of hiPSC‐derived myogenic progenitors. Transcriptomic analyses demonstrate that this property is conserved across species and multiple hiPSC lines, consistent with results from single cell motility profiling. Treated cells showed enhanced trans‐endothelial migration in transwell assays. Finally, increased motility was detected in a novel humanised assay to study cell migration using 3D artificial muscles, harnessing advanced tissue modelling to move hiPSCs closer to future muscle gene and cell therapies.
format Online
Article
Text
id pubmed-9549733
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-95497332022-10-14 Assessing and enhancing migration of human myogenic progenitors using directed iPS cell differentiation and advanced tissue modelling Choi, SungWoo Ferrari, Giulia Moyle, Louise A Mackinlay, Kirsty Naouar, Naira Jalal, Salma Benedetti, Sara Wells, Christine Muntoni, Francesco Tedesco, Francesco Saverio EMBO Mol Med Articles Muscle satellite stem cells (MuSCs) are responsible for skeletal muscle growth and regeneration. Despite their differentiation potential, human MuSCs have limited in vitro expansion and in vivo migration capacity, limiting their use in cell therapies for diseases affecting multiple skeletal muscles. Several protocols have been developed to derive MuSC‐like progenitors from human induced pluripotent stem (iPS) cells (hiPSCs) to establish a source of myogenic cells with controllable proliferation and differentiation. However, current hiPSC myogenic derivatives also suffer from limitations of cell migration, ultimately delaying their clinical translation. Here we use a multi‐disciplinary approach including bioinformatics and tissue engineering to show that DLL4 and PDGF‐BB improve migration of hiPSC‐derived myogenic progenitors. Transcriptomic analyses demonstrate that this property is conserved across species and multiple hiPSC lines, consistent with results from single cell motility profiling. Treated cells showed enhanced trans‐endothelial migration in transwell assays. Finally, increased motility was detected in a novel humanised assay to study cell migration using 3D artificial muscles, harnessing advanced tissue modelling to move hiPSCs closer to future muscle gene and cell therapies. John Wiley and Sons Inc. 2022-09-26 /pmc/articles/PMC9549733/ /pubmed/36161772 http://dx.doi.org/10.15252/emmm.202114526 Text en © 2022 The Authors. Published under the terms of the CC BY 4.0 license. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Choi, SungWoo
Ferrari, Giulia
Moyle, Louise A
Mackinlay, Kirsty
Naouar, Naira
Jalal, Salma
Benedetti, Sara
Wells, Christine
Muntoni, Francesco
Tedesco, Francesco Saverio
Assessing and enhancing migration of human myogenic progenitors using directed iPS cell differentiation and advanced tissue modelling
title Assessing and enhancing migration of human myogenic progenitors using directed iPS cell differentiation and advanced tissue modelling
title_full Assessing and enhancing migration of human myogenic progenitors using directed iPS cell differentiation and advanced tissue modelling
title_fullStr Assessing and enhancing migration of human myogenic progenitors using directed iPS cell differentiation and advanced tissue modelling
title_full_unstemmed Assessing and enhancing migration of human myogenic progenitors using directed iPS cell differentiation and advanced tissue modelling
title_short Assessing and enhancing migration of human myogenic progenitors using directed iPS cell differentiation and advanced tissue modelling
title_sort assessing and enhancing migration of human myogenic progenitors using directed ips cell differentiation and advanced tissue modelling
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9549733/
https://www.ncbi.nlm.nih.gov/pubmed/36161772
http://dx.doi.org/10.15252/emmm.202114526
work_keys_str_mv AT choisungwoo assessingandenhancingmigrationofhumanmyogenicprogenitorsusingdirectedipscelldifferentiationandadvancedtissuemodelling
AT ferrarigiulia assessingandenhancingmigrationofhumanmyogenicprogenitorsusingdirectedipscelldifferentiationandadvancedtissuemodelling
AT moylelouisea assessingandenhancingmigrationofhumanmyogenicprogenitorsusingdirectedipscelldifferentiationandadvancedtissuemodelling
AT mackinlaykirsty assessingandenhancingmigrationofhumanmyogenicprogenitorsusingdirectedipscelldifferentiationandadvancedtissuemodelling
AT naouarnaira assessingandenhancingmigrationofhumanmyogenicprogenitorsusingdirectedipscelldifferentiationandadvancedtissuemodelling
AT jalalsalma assessingandenhancingmigrationofhumanmyogenicprogenitorsusingdirectedipscelldifferentiationandadvancedtissuemodelling
AT benedettisara assessingandenhancingmigrationofhumanmyogenicprogenitorsusingdirectedipscelldifferentiationandadvancedtissuemodelling
AT wellschristine assessingandenhancingmigrationofhumanmyogenicprogenitorsusingdirectedipscelldifferentiationandadvancedtissuemodelling
AT muntonifrancesco assessingandenhancingmigrationofhumanmyogenicprogenitorsusingdirectedipscelldifferentiationandadvancedtissuemodelling
AT tedescofrancescosaverio assessingandenhancingmigrationofhumanmyogenicprogenitorsusingdirectedipscelldifferentiationandadvancedtissuemodelling